Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5562 and Pf6N2E2_5563 are separated by 201 nucleotides Pf6N2E2_5563 and Pf6N2E2_5564 are separated by 50 nucleotides Pf6N2E2_5564 and Pf6N2E2_5565 are separated by 19 nucleotides
Pf6N2E2_5562 - Metallo-beta-lactamase superfamily protein PA0057, at 6,380,589 to 6,381,467
_5562
Pf6N2E2_5563 - Rrf2-linked NADH-flavin reductase, at 6,381,669 to 6,382,283
_5563
Pf6N2E2_5564 - Membrane protein glpM, at 6,382,334 to 6,382,672
_5564
Pf6N2E2_5565 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 6,382,692 to 6,384,020
_5565
Position (kb)
6381
6382
6383 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6380.685 kb on + strand, within Pf6N2E2_5562 at 6380.685 kb on + strand, within Pf6N2E2_5562 at 6380.685 kb on + strand, within Pf6N2E2_5562 at 6380.685 kb on + strand, within Pf6N2E2_5562 at 6380.686 kb on - strand, within Pf6N2E2_5562 at 6380.686 kb on - strand, within Pf6N2E2_5562 at 6380.759 kb on - strand, within Pf6N2E2_5562 at 6380.838 kb on + strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.839 kb on - strand, within Pf6N2E2_5562 at 6380.862 kb on + strand, within Pf6N2E2_5562 at 6380.862 kb on + strand, within Pf6N2E2_5562 at 6380.862 kb on + strand, within Pf6N2E2_5562 at 6380.863 kb on - strand, within Pf6N2E2_5562 at 6380.865 kb on + strand, within Pf6N2E2_5562 at 6380.865 kb on + strand, within Pf6N2E2_5562 at 6380.865 kb on + strand, within Pf6N2E2_5562 at 6380.865 kb on + strand, within Pf6N2E2_5562 at 6380.865 kb on + strand, within Pf6N2E2_5562 at 6380.866 kb on - strand, within Pf6N2E2_5562 at 6380.866 kb on - strand, within Pf6N2E2_5562 at 6380.866 kb on - strand, within Pf6N2E2_5562 at 6380.970 kb on + strand, within Pf6N2E2_5562 at 6381.270 kb on + strand, within Pf6N2E2_5562 at 6381.270 kb on + strand, within Pf6N2E2_5562 at 6381.270 kb on + strand, within Pf6N2E2_5562 at 6381.271 kb on - strand, within Pf6N2E2_5562 at 6381.271 kb on - strand, within Pf6N2E2_5562 at 6381.271 kb on - strand, within Pf6N2E2_5562 at 6381.321 kb on + strand, within Pf6N2E2_5562 at 6381.322 kb on - strand, within Pf6N2E2_5562 at 6381.391 kb on + strand at 6381.391 kb on + strand at 6381.391 kb on + strand at 6381.391 kb on + strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.392 kb on - strand at 6381.480 kb on - strand at 6381.511 kb on + strand at 6381.511 kb on + strand at 6381.511 kb on + strand at 6381.556 kb on + strand at 6381.556 kb on + strand at 6381.556 kb on + strand at 6381.556 kb on + strand at 6381.557 kb on - strand at 6381.638 kb on + strand at 6381.638 kb on + strand at 6381.638 kb on + strand at 6381.638 kb on + strand at 6381.638 kb on + strand at 6381.639 kb on - strand at 6381.639 kb on - strand at 6381.639 kb on - strand at 6381.639 kb on - strand at 6381.639 kb on - strand at 6381.639 kb on - strand at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.820 kb on + strand, within Pf6N2E2_5563 at 6381.821 kb on - strand, within Pf6N2E2_5563 at 6381.821 kb on - strand, within Pf6N2E2_5563 at 6381.821 kb on - strand, within Pf6N2E2_5563 at 6381.821 kb on - strand, within Pf6N2E2_5563 at 6381.987 kb on - strand, within Pf6N2E2_5563 at 6382.026 kb on + strand, within Pf6N2E2_5563 at 6382.026 kb on + strand, within Pf6N2E2_5563 at 6382.026 kb on + strand, within Pf6N2E2_5563 at 6382.027 kb on - strand, within Pf6N2E2_5563 at 6382.066 kb on + strand, within Pf6N2E2_5563 at 6382.066 kb on + strand, within Pf6N2E2_5563 at 6382.066 kb on + strand, within Pf6N2E2_5563 at 6382.066 kb on + strand, within Pf6N2E2_5563 at 6382.067 kb on - strand, within Pf6N2E2_5563 at 6382.067 kb on - strand, within Pf6N2E2_5563 at 6382.067 kb on - strand, within Pf6N2E2_5563 at 6382.067 kb on - strand, within Pf6N2E2_5563 at 6382.067 kb on - strand, within Pf6N2E2_5563 at 6382.331 kb on + strand at 6382.331 kb on + strand at 6382.332 kb on - strand at 6382.332 kb on - strand at 6382.332 kb on - strand at 6382.332 kb on - strand at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.443 kb on + strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.444 kb on - strand, within Pf6N2E2_5564 at 6382.458 kb on + strand, within Pf6N2E2_5564 at 6382.459 kb on - strand, within Pf6N2E2_5564 at 6382.467 kb on + strand, within Pf6N2E2_5564 at 6382.467 kb on + strand, within Pf6N2E2_5564 at 6382.467 kb on + strand, within Pf6N2E2_5564 at 6382.468 kb on - strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.542 kb on + strand, within Pf6N2E2_5564 at 6382.543 kb on - strand, within Pf6N2E2_5564 at 6382.543 kb on - strand, within Pf6N2E2_5564 at 6382.543 kb on - strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.593 kb on + strand, within Pf6N2E2_5564 at 6382.594 kb on - strand, within Pf6N2E2_5564 at 6382.594 kb on - strand, within Pf6N2E2_5564 at 6382.594 kb on - strand, within Pf6N2E2_5564 at 6382.594 kb on - strand, within Pf6N2E2_5564 at 6382.594 kb on - strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.596 kb on + strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.597 kb on - strand, within Pf6N2E2_5564 at 6382.709 kb on - strand at 6382.932 kb on + strand, within Pf6N2E2_5565 at 6382.933 kb on - strand, within Pf6N2E2_5565 at 6382.933 kb on - strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.110 kb on + strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.111 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565 at 6383.221 kb on - strand, within Pf6N2E2_5565
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,380,685 + Pf6N2E2_5562 0.11 +0.4 6,380,685 + Pf6N2E2_5562 0.11 +0.2 6,380,685 + Pf6N2E2_5562 0.11 +2.7 6,380,685 + Pf6N2E2_5562 0.11 -1.4 6,380,686 - Pf6N2E2_5562 0.11 -0.1 6,380,686 - Pf6N2E2_5562 0.11 -0.6 6,380,759 - Pf6N2E2_5562 0.19 +0.8 6,380,838 + Pf6N2E2_5562 0.28 -0.6 6,380,839 - Pf6N2E2_5562 0.28 -1.2 6,380,839 - Pf6N2E2_5562 0.28 -1.2 6,380,839 - Pf6N2E2_5562 0.28 +0.4 6,380,839 - Pf6N2E2_5562 0.28 -1.4 6,380,839 - Pf6N2E2_5562 0.28 -0.1 6,380,839 - Pf6N2E2_5562 0.28 +0.0 6,380,839 - Pf6N2E2_5562 0.28 +0.8 6,380,839 - Pf6N2E2_5562 0.28 -0.9 6,380,839 - Pf6N2E2_5562 0.28 +0.2 6,380,862 + Pf6N2E2_5562 0.31 -1.2 6,380,862 + Pf6N2E2_5562 0.31 +0.6 6,380,862 + Pf6N2E2_5562 0.31 -1.1 6,380,863 - Pf6N2E2_5562 0.31 +1.1 6,380,865 + Pf6N2E2_5562 0.31 -1.4 6,380,865 + Pf6N2E2_5562 0.31 -1.0 6,380,865 + Pf6N2E2_5562 0.31 +0.3 6,380,865 + Pf6N2E2_5562 0.31 +0.1 6,380,865 + Pf6N2E2_5562 0.31 -2.0 6,380,866 - Pf6N2E2_5562 0.32 +0.2 6,380,866 - Pf6N2E2_5562 0.32 -1.3 6,380,866 - Pf6N2E2_5562 0.32 +0.5 6,380,970 + Pf6N2E2_5562 0.43 +0.9 6,381,270 + Pf6N2E2_5562 0.77 +0.6 6,381,270 + Pf6N2E2_5562 0.77 +0.3 6,381,270 + Pf6N2E2_5562 0.77 +0.7 6,381,271 - Pf6N2E2_5562 0.78 +2.6 6,381,271 - Pf6N2E2_5562 0.78 +0.7 6,381,271 - Pf6N2E2_5562 0.78 -0.1 6,381,321 + Pf6N2E2_5562 0.83 -1.1 6,381,322 - Pf6N2E2_5562 0.83 -0.2 6,381,391 + -0.6 6,381,391 + -0.3 6,381,391 + -1.4 6,381,391 + +0.2 6,381,392 - -2.1 6,381,392 - +0.0 6,381,392 - -0.1 6,381,392 - +0.8 6,381,392 - +1.9 6,381,392 - +0.2 6,381,392 - -0.4 6,381,392 - +1.1 6,381,480 - +1.0 6,381,511 + +0.8 6,381,511 + -0.5 6,381,511 + +0.9 6,381,556 + +1.3 6,381,556 + -0.8 6,381,556 + +0.3 6,381,556 + +0.1 6,381,557 - +0.9 6,381,638 + +1.1 6,381,638 + +2.3 6,381,638 + +0.1 6,381,638 + +0.6 6,381,638 + +1.7 6,381,639 - -0.2 6,381,639 - +1.0 6,381,639 - -1.0 6,381,639 - +0.7 6,381,639 - -0.1 6,381,639 - -1.8 6,381,820 + Pf6N2E2_5563 0.25 +0.8 6,381,820 + Pf6N2E2_5563 0.25 -0.4 6,381,820 + Pf6N2E2_5563 0.25 +0.6 6,381,820 + Pf6N2E2_5563 0.25 +0.7 6,381,820 + Pf6N2E2_5563 0.25 +1.0 6,381,820 + Pf6N2E2_5563 0.25 -1.2 6,381,820 + Pf6N2E2_5563 0.25 -0.4 6,381,820 + Pf6N2E2_5563 0.25 +1.6 6,381,820 + Pf6N2E2_5563 0.25 -0.5 6,381,820 + Pf6N2E2_5563 0.25 +0.6 6,381,820 + Pf6N2E2_5563 0.25 -0.1 6,381,820 + Pf6N2E2_5563 0.25 +0.2 6,381,821 - Pf6N2E2_5563 0.25 +0.6 6,381,821 - Pf6N2E2_5563 0.25 +0.2 6,381,821 - Pf6N2E2_5563 0.25 +0.4 6,381,821 - Pf6N2E2_5563 0.25 -1.1 6,381,987 - Pf6N2E2_5563 0.52 -0.8 6,382,026 + Pf6N2E2_5563 0.58 +0.5 6,382,026 + Pf6N2E2_5563 0.58 +0.1 6,382,026 + Pf6N2E2_5563 0.58 +0.6 6,382,027 - Pf6N2E2_5563 0.58 +1.7 6,382,066 + Pf6N2E2_5563 0.65 -1.6 6,382,066 + Pf6N2E2_5563 0.65 -0.1 6,382,066 + Pf6N2E2_5563 0.65 -0.2 6,382,066 + Pf6N2E2_5563 0.65 -0.0 6,382,067 - Pf6N2E2_5563 0.65 -0.8 6,382,067 - Pf6N2E2_5563 0.65 +1.5 6,382,067 - Pf6N2E2_5563 0.65 +1.6 6,382,067 - Pf6N2E2_5563 0.65 -1.4 6,382,067 - Pf6N2E2_5563 0.65 +0.3 6,382,331 + +1.1 6,382,331 + +0.6 6,382,332 - -1.4 6,382,332 - -0.1 6,382,332 - +3.4 6,382,332 - -0.6 6,382,443 + Pf6N2E2_5564 0.32 -0.5 6,382,443 + Pf6N2E2_5564 0.32 +1.1 6,382,443 + Pf6N2E2_5564 0.32 -0.2 6,382,443 + Pf6N2E2_5564 0.32 -0.2 6,382,443 + Pf6N2E2_5564 0.32 -0.6 6,382,443 + Pf6N2E2_5564 0.32 -0.3 6,382,443 + Pf6N2E2_5564 0.32 -0.4 6,382,443 + Pf6N2E2_5564 0.32 -2.1 6,382,443 + Pf6N2E2_5564 0.32 +1.0 6,382,444 - Pf6N2E2_5564 0.32 -1.8 6,382,444 - Pf6N2E2_5564 0.32 -0.3 6,382,444 - Pf6N2E2_5564 0.32 -1.6 6,382,444 - Pf6N2E2_5564 0.32 -2.5 6,382,444 - Pf6N2E2_5564 0.32 -0.2 6,382,444 - Pf6N2E2_5564 0.32 +1.9 6,382,458 + Pf6N2E2_5564 0.37 -0.4 6,382,459 - Pf6N2E2_5564 0.37 -0.2 6,382,467 + Pf6N2E2_5564 0.39 +0.6 6,382,467 + Pf6N2E2_5564 0.39 -1.6 6,382,467 + Pf6N2E2_5564 0.39 -0.2 6,382,468 - Pf6N2E2_5564 0.40 -0.2 6,382,542 + Pf6N2E2_5564 0.61 +1.1 6,382,542 + Pf6N2E2_5564 0.61 +1.1 6,382,542 + Pf6N2E2_5564 0.61 -0.3 6,382,542 + Pf6N2E2_5564 0.61 -0.5 6,382,542 + Pf6N2E2_5564 0.61 -0.0 6,382,542 + Pf6N2E2_5564 0.61 +1.3 6,382,542 + Pf6N2E2_5564 0.61 +1.1 6,382,542 + Pf6N2E2_5564 0.61 -1.8 6,382,542 + Pf6N2E2_5564 0.61 -0.8 6,382,542 + Pf6N2E2_5564 0.61 +1.0 6,382,542 + Pf6N2E2_5564 0.61 +0.2 6,382,542 + Pf6N2E2_5564 0.61 +1.8 6,382,543 - Pf6N2E2_5564 0.62 +0.8 6,382,543 - Pf6N2E2_5564 0.62 -1.8 6,382,543 - Pf6N2E2_5564 0.62 +0.2 6,382,593 + Pf6N2E2_5564 0.76 +0.4 6,382,593 + Pf6N2E2_5564 0.76 +0.2 6,382,593 + Pf6N2E2_5564 0.76 -0.1 6,382,593 + Pf6N2E2_5564 0.76 +1.1 6,382,593 + Pf6N2E2_5564 0.76 +0.9 6,382,593 + Pf6N2E2_5564 0.76 -1.5 6,382,593 + Pf6N2E2_5564 0.76 -2.9 6,382,594 - Pf6N2E2_5564 0.77 +0.7 6,382,594 - Pf6N2E2_5564 0.77 +1.2 6,382,594 - Pf6N2E2_5564 0.77 +0.3 6,382,594 - Pf6N2E2_5564 0.77 -0.0 6,382,594 - Pf6N2E2_5564 0.77 +0.6 6,382,596 + Pf6N2E2_5564 0.77 +0.5 6,382,596 + Pf6N2E2_5564 0.77 -1.1 6,382,596 + Pf6N2E2_5564 0.77 +0.7 6,382,596 + Pf6N2E2_5564 0.77 -0.4 6,382,596 + Pf6N2E2_5564 0.77 -1.5 6,382,596 + Pf6N2E2_5564 0.77 +1.2 6,382,596 + Pf6N2E2_5564 0.77 -0.5 6,382,596 + Pf6N2E2_5564 0.77 -0.2 6,382,596 + Pf6N2E2_5564 0.77 +1.3 6,382,597 - Pf6N2E2_5564 0.78 +0.2 6,382,597 - Pf6N2E2_5564 0.78 +1.2 6,382,597 - Pf6N2E2_5564 0.78 +0.8 6,382,597 - Pf6N2E2_5564 0.78 +0.5 6,382,597 - Pf6N2E2_5564 0.78 +0.1 6,382,597 - Pf6N2E2_5564 0.78 +0.8 6,382,597 - Pf6N2E2_5564 0.78 -1.1 6,382,709 - -1.6 6,382,932 + Pf6N2E2_5565 0.18 +1.0 6,382,933 - Pf6N2E2_5565 0.18 +0.4 6,382,933 - Pf6N2E2_5565 0.18 +0.3 6,383,110 + Pf6N2E2_5565 0.31 +1.4 6,383,110 + Pf6N2E2_5565 0.31 +1.5 6,383,110 + Pf6N2E2_5565 0.31 +0.2 6,383,110 + Pf6N2E2_5565 0.31 -0.2 6,383,110 + Pf6N2E2_5565 0.31 -1.2 6,383,110 + Pf6N2E2_5565 0.31 -0.6 6,383,110 + Pf6N2E2_5565 0.31 +0.5 6,383,110 + Pf6N2E2_5565 0.31 -1.8 6,383,110 + Pf6N2E2_5565 0.31 +0.3 6,383,111 - Pf6N2E2_5565 0.32 -0.6 6,383,111 - Pf6N2E2_5565 0.32 -1.2 6,383,111 - Pf6N2E2_5565 0.32 +0.8 6,383,111 - Pf6N2E2_5565 0.32 -0.1 6,383,221 - Pf6N2E2_5565 0.40 +2.3 6,383,221 - Pf6N2E2_5565 0.40 +1.1
Or see this region's nucleotide sequence