Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_548 and Pf6N2E2_549 are separated by 620 nucleotides Pf6N2E2_549 and Pf6N2E2_550 are separated by 1 nucleotides
Pf6N2E2_548 - VgrG protein, at 616,714 to 618,723
_548
Pf6N2E2_549 - ClpB protein, at 619,344 to 621,887
_549
Pf6N2E2_550 - Uncharacterized protein ImpH/VasB, at 621,889 to 622,914
_550
Pf6N2E2_551 - Protein ImpG/VasA, at 622,878 to 624,668
_551
Position (kb) 619
620
621
622 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 618.452 kb on + strand, within Pf6N2E2_548 at 618.659 kb on + strand at 618.659 kb on + strand at 618.660 kb on - strand at 618.743 kb on - strand at 618.764 kb on + strand at 618.764 kb on + strand at 618.784 kb on + strand at 618.787 kb on + strand at 618.787 kb on + strand at 618.787 kb on + strand at 618.788 kb on - strand at 618.818 kb on - strand at 618.874 kb on + strand at 618.875 kb on - strand at 618.875 kb on - strand at 618.907 kb on + strand at 618.907 kb on + strand at 618.907 kb on + strand at 618.907 kb on + strand at 618.908 kb on - strand at 618.908 kb on - strand at 618.908 kb on - strand at 618.908 kb on - strand at 618.908 kb on - strand at 618.908 kb on - strand at 618.911 kb on + strand at 618.997 kb on + strand at 618.998 kb on - strand at 618.998 kb on - strand at 618.999 kb on + strand at 618.999 kb on + strand at 618.999 kb on + strand at 618.999 kb on + strand at 618.999 kb on + strand at 619.000 kb on - strand at 619.000 kb on - strand at 619.006 kb on + strand at 619.006 kb on + strand at 619.007 kb on - strand at 619.068 kb on - strand at 619.123 kb on + strand at 619.124 kb on - strand at 619.124 kb on - strand at 619.124 kb on - strand at 619.154 kb on - strand at 619.154 kb on - strand at 619.154 kb on - strand at 619.154 kb on - strand at 619.156 kb on + strand at 619.157 kb on - strand at 619.157 kb on - strand at 619.777 kb on + strand, within Pf6N2E2_549 at 619.777 kb on + strand, within Pf6N2E2_549 at 619.778 kb on - strand, within Pf6N2E2_549 at 619.778 kb on - strand, within Pf6N2E2_549 at 619.778 kb on - strand, within Pf6N2E2_549 at 619.778 kb on - strand, within Pf6N2E2_549 at 619.837 kb on + strand, within Pf6N2E2_549 at 619.892 kb on - strand, within Pf6N2E2_549 at 619.939 kb on + strand, within Pf6N2E2_549 at 619.982 kb on - strand, within Pf6N2E2_549 at 619.982 kb on - strand, within Pf6N2E2_549 at 620.003 kb on - strand, within Pf6N2E2_549 at 620.704 kb on + strand, within Pf6N2E2_549 at 620.705 kb on - strand, within Pf6N2E2_549 at 620.705 kb on - strand, within Pf6N2E2_549 at 621.352 kb on + strand, within Pf6N2E2_549 at 621.353 kb on - strand, within Pf6N2E2_549 at 621.353 kb on - strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.451 kb on - strand, within Pf6N2E2_549 at 621.582 kb on + strand, within Pf6N2E2_549 at 621.583 kb on - strand, within Pf6N2E2_549 at 621.793 kb on + strand at 621.793 kb on + strand at 621.794 kb on - strand at 622.061 kb on + strand, within Pf6N2E2_550 at 622.071 kb on - strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.620 kb on - strand, within Pf6N2E2_550
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 618,452 + Pf6N2E2_548 0.86 -0.1 618,659 + +0.9 618,659 + +0.9 618,660 - +1.2 618,743 - -2.2 618,764 + -0.7 618,764 + +0.0 618,784 + -1.5 618,787 + -2.5 618,787 + +0.4 618,787 + +0.4 618,788 - +1.5 618,818 - +0.9 618,874 + +0.8 618,875 - +0.3 618,875 - +2.0 618,907 + -0.8 618,907 + -0.4 618,907 + -0.7 618,907 + -0.4 618,908 - +0.7 618,908 - -0.2 618,908 - -0.5 618,908 - -1.9 618,908 - +0.3 618,908 - -2.0 618,911 + -1.3 618,997 + +1.0 618,998 - -1.2 618,998 - -1.5 618,999 + -0.1 618,999 + +0.9 618,999 + -0.8 618,999 + -0.4 618,999 + +1.7 619,000 - -0.3 619,000 - -0.8 619,006 + +0.5 619,006 + +0.3 619,007 - +0.4 619,068 - -0.4 619,123 + +1.4 619,124 - -2.4 619,124 - -1.5 619,124 - +1.1 619,154 - +0.4 619,154 - -3.2 619,154 - -2.1 619,154 - -0.2 619,156 + -0.7 619,157 - -0.5 619,157 - -0.9 619,777 + Pf6N2E2_549 0.17 -1.1 619,777 + Pf6N2E2_549 0.17 +1.5 619,778 - Pf6N2E2_549 0.17 -0.4 619,778 - Pf6N2E2_549 0.17 +0.4 619,778 - Pf6N2E2_549 0.17 -0.2 619,778 - Pf6N2E2_549 0.17 +0.7 619,837 + Pf6N2E2_549 0.19 +0.5 619,892 - Pf6N2E2_549 0.22 +1.1 619,939 + Pf6N2E2_549 0.23 +0.2 619,982 - Pf6N2E2_549 0.25 -1.4 619,982 - Pf6N2E2_549 0.25 -0.0 620,003 - Pf6N2E2_549 0.26 -0.1 620,704 + Pf6N2E2_549 0.53 +1.2 620,705 - Pf6N2E2_549 0.53 +0.4 620,705 - Pf6N2E2_549 0.53 +0.4 621,352 + Pf6N2E2_549 0.79 +1.2 621,353 - Pf6N2E2_549 0.79 -1.4 621,353 - Pf6N2E2_549 0.79 -0.1 621,450 + Pf6N2E2_549 0.83 -0.2 621,450 + Pf6N2E2_549 0.83 +0.6 621,450 + Pf6N2E2_549 0.83 -2.2 621,451 - Pf6N2E2_549 0.83 -1.3 621,582 + Pf6N2E2_549 0.88 +0.0 621,583 - Pf6N2E2_549 0.88 -0.7 621,793 + -2.4 621,793 + -0.4 621,794 - +0.3 622,061 + Pf6N2E2_550 0.17 +1.0 622,071 - Pf6N2E2_550 0.18 -1.1 622,520 + Pf6N2E2_550 0.62 +0.2 622,520 + Pf6N2E2_550 0.62 -0.5 622,520 + Pf6N2E2_550 0.62 -1.0 622,521 - Pf6N2E2_550 0.62 +0.8 622,521 - Pf6N2E2_550 0.62 +1.2 622,521 - Pf6N2E2_550 0.62 -0.9 622,620 - Pf6N2E2_550 0.71 -1.1
Or see this region's nucleotide sequence