Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5193 and Pf6N2E2_5194 are separated by 106 nucleotides Pf6N2E2_5194 and Pf6N2E2_5195 are separated by 95 nucleotides Pf6N2E2_5195 and Pf6N2E2_5196 are separated by 7 nucleotides Pf6N2E2_5196 and Pf6N2E2_5197 overlap by 19 nucleotides Pf6N2E2_5197 and Pf6N2E2_5198 are separated by 1 nucleotides
Pf6N2E2_5193 - Short chain fatty acids transporter, at 5,988,398 to 5,989,882
_5193
Pf6N2E2_5194 - Methyl-accepting chemotaxis protein, at 5,989,989 to 5,991,350
_5194
Pf6N2E2_5195 - hypothetical protein, at 5,991,446 to 5,992,000
_5195
Pf6N2E2_5196 - Transcriptional regulator, Cro/CI family, at 5,992,008 to 5,992,241
_5196
Pf6N2E2_5197 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 5,992,223 to 5,992,345
_5197
Pf6N2E2_5198 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 5,992,347 to 5,993,582
_5198
Position (kb) 5989
5990
5991
5992 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5989.362 kb on + strand, within Pf6N2E2_5193 at 5989.362 kb on + strand, within Pf6N2E2_5193 at 5989.363 kb on - strand, within Pf6N2E2_5193 at 5989.386 kb on + strand, within Pf6N2E2_5193 at 5989.823 kb on + strand at 5989.823 kb on + strand at 5989.823 kb on + strand at 5989.824 kb on - strand at 5989.834 kb on + strand at 5989.834 kb on + strand at 5989.835 kb on - strand at 5989.835 kb on - strand at 5989.835 kb on - strand at 5989.835 kb on - strand at 5989.886 kb on + strand at 5989.886 kb on + strand at 5989.886 kb on + strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.887 kb on - strand at 5989.969 kb on - strand at 5990.008 kb on + strand at 5990.008 kb on + strand at 5990.009 kb on - strand at 5990.039 kb on + strand at 5990.039 kb on + strand at 5990.039 kb on + strand at 5990.377 kb on + strand, within Pf6N2E2_5194 at 5990.377 kb on + strand, within Pf6N2E2_5194 at 5990.378 kb on - strand, within Pf6N2E2_5194 at 5990.378 kb on - strand, within Pf6N2E2_5194 at 5990.415 kb on + strand, within Pf6N2E2_5194 at 5990.416 kb on - strand, within Pf6N2E2_5194 at 5990.416 kb on - strand, within Pf6N2E2_5194 at 5990.416 kb on - strand, within Pf6N2E2_5194 at 5990.416 kb on - strand, within Pf6N2E2_5194 at 5990.524 kb on + strand, within Pf6N2E2_5194 at 5990.524 kb on + strand, within Pf6N2E2_5194 at 5990.525 kb on - strand, within Pf6N2E2_5194 at 5990.525 kb on - strand, within Pf6N2E2_5194 at 5990.525 kb on - strand, within Pf6N2E2_5194 at 5990.525 kb on - strand, within Pf6N2E2_5194 at 5990.619 kb on + strand, within Pf6N2E2_5194 at 5990.620 kb on - strand, within Pf6N2E2_5194 at 5990.620 kb on - strand, within Pf6N2E2_5194 at 5990.620 kb on - strand, within Pf6N2E2_5194 at 5990.620 kb on - strand, within Pf6N2E2_5194 at 5990.634 kb on + strand, within Pf6N2E2_5194 at 5990.635 kb on - strand, within Pf6N2E2_5194 at 5990.693 kb on + strand, within Pf6N2E2_5194 at 5990.694 kb on - strand, within Pf6N2E2_5194 at 5990.733 kb on + strand, within Pf6N2E2_5194 at 5990.733 kb on + strand, within Pf6N2E2_5194 at 5990.733 kb on + strand, within Pf6N2E2_5194 at 5990.733 kb on + strand, within Pf6N2E2_5194 at 5990.766 kb on + strand, within Pf6N2E2_5194 at 5990.766 kb on + strand, within Pf6N2E2_5194 at 5990.766 kb on + strand, within Pf6N2E2_5194 at 5990.766 kb on + strand, within Pf6N2E2_5194 at 5990.766 kb on + strand, within Pf6N2E2_5194 at 5990.996 kb on + strand, within Pf6N2E2_5194 at 5991.138 kb on - strand, within Pf6N2E2_5194 at 5991.349 kb on - strand at 5991.349 kb on - strand at 5991.426 kb on + strand at 5991.426 kb on + strand at 5991.427 kb on - strand at 5991.427 kb on - strand at 5991.451 kb on + strand at 5991.452 kb on - strand at 5991.470 kb on + strand at 5991.670 kb on + strand, within Pf6N2E2_5195 at 5991.671 kb on - strand, within Pf6N2E2_5195 at 5991.728 kb on + strand, within Pf6N2E2_5195 at 5991.743 kb on + strand, within Pf6N2E2_5195 at 5991.767 kb on + strand, within Pf6N2E2_5195 at 5991.767 kb on + strand, within Pf6N2E2_5195 at 5991.767 kb on + strand, within Pf6N2E2_5195 at 5991.768 kb on - strand, within Pf6N2E2_5195 at 5991.768 kb on - strand, within Pf6N2E2_5195 at 5991.889 kb on + strand, within Pf6N2E2_5195 at 5991.890 kb on - strand, within Pf6N2E2_5195 at 5991.932 kb on - strand, within Pf6N2E2_5195 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.033 kb on + strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.034 kb on - strand, within Pf6N2E2_5196 at 5992.082 kb on - strand, within Pf6N2E2_5196 at 5992.133 kb on + strand, within Pf6N2E2_5196 at 5992.133 kb on + strand, within Pf6N2E2_5196 at 5992.133 kb on + strand, within Pf6N2E2_5196 at 5992.134 kb on - strand, within Pf6N2E2_5196 at 5992.134 kb on - strand, within Pf6N2E2_5196 at 5992.134 kb on - strand, within Pf6N2E2_5196 at 5992.134 kb on - strand, within Pf6N2E2_5196 at 5992.135 kb on + strand, within Pf6N2E2_5196 at 5992.135 kb on + strand, within Pf6N2E2_5196 at 5992.135 kb on + strand, within Pf6N2E2_5196 at 5992.135 kb on + strand, within Pf6N2E2_5196 at 5992.136 kb on - strand, within Pf6N2E2_5196 at 5992.136 kb on - strand, within Pf6N2E2_5196 at 5992.136 kb on - strand, within Pf6N2E2_5196 at 5992.136 kb on - strand, within Pf6N2E2_5196 at 5992.167 kb on + strand, within Pf6N2E2_5196 at 5992.167 kb on + strand, within Pf6N2E2_5196 at 5992.168 kb on - strand, within Pf6N2E2_5196 at 5992.168 kb on - strand, within Pf6N2E2_5196 at 5992.168 kb on - strand, within Pf6N2E2_5196 at 5992.168 kb on - strand, within Pf6N2E2_5196 at 5992.200 kb on + strand, within Pf6N2E2_5196 at 5992.201 kb on - strand, within Pf6N2E2_5196
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,989,362 + Pf6N2E2_5193 0.65 +1.0 5,989,362 + Pf6N2E2_5193 0.65 +1.9 5,989,363 - Pf6N2E2_5193 0.65 -1.4 5,989,386 + Pf6N2E2_5193 0.67 -0.8 5,989,823 + -0.4 5,989,823 + +0.8 5,989,823 + +2.3 5,989,824 - +0.6 5,989,834 + +1.7 5,989,834 + +1.3 5,989,835 - -2.6 5,989,835 - +0.9 5,989,835 - -1.6 5,989,835 - -2.7 5,989,886 + -0.0 5,989,886 + -1.9 5,989,886 + -0.9 5,989,887 - +0.4 5,989,887 - -0.6 5,989,887 - +1.2 5,989,887 - -0.2 5,989,887 - -1.1 5,989,887 - -1.3 5,989,887 - -0.9 5,989,887 - -0.7 5,989,887 - -0.2 5,989,887 - +0.7 5,989,887 - -2.0 5,989,969 - -0.1 5,990,008 + +1.3 5,990,008 + +1.5 5,990,009 - -2.5 5,990,039 + +1.5 5,990,039 + -1.0 5,990,039 + -1.0 5,990,377 + Pf6N2E2_5194 0.28 -0.2 5,990,377 + Pf6N2E2_5194 0.28 -0.1 5,990,378 - Pf6N2E2_5194 0.29 +1.3 5,990,378 - Pf6N2E2_5194 0.29 -0.8 5,990,415 + Pf6N2E2_5194 0.31 -2.4 5,990,416 - Pf6N2E2_5194 0.31 +0.2 5,990,416 - Pf6N2E2_5194 0.31 +1.3 5,990,416 - Pf6N2E2_5194 0.31 -0.8 5,990,416 - Pf6N2E2_5194 0.31 -0.4 5,990,524 + Pf6N2E2_5194 0.39 +1.2 5,990,524 + Pf6N2E2_5194 0.39 +0.5 5,990,525 - Pf6N2E2_5194 0.39 -1.6 5,990,525 - Pf6N2E2_5194 0.39 +0.4 5,990,525 - Pf6N2E2_5194 0.39 -1.3 5,990,525 - Pf6N2E2_5194 0.39 -0.9 5,990,619 + Pf6N2E2_5194 0.46 -3.0 5,990,620 - Pf6N2E2_5194 0.46 -0.2 5,990,620 - Pf6N2E2_5194 0.46 -0.8 5,990,620 - Pf6N2E2_5194 0.46 -0.0 5,990,620 - Pf6N2E2_5194 0.46 -1.0 5,990,634 + Pf6N2E2_5194 0.47 -0.2 5,990,635 - Pf6N2E2_5194 0.47 -2.0 5,990,693 + Pf6N2E2_5194 0.52 +0.5 5,990,694 - Pf6N2E2_5194 0.52 +0.0 5,990,733 + Pf6N2E2_5194 0.55 -0.5 5,990,733 + Pf6N2E2_5194 0.55 -0.1 5,990,733 + Pf6N2E2_5194 0.55 +1.8 5,990,733 + Pf6N2E2_5194 0.55 -1.6 5,990,766 + Pf6N2E2_5194 0.57 -1.1 5,990,766 + Pf6N2E2_5194 0.57 +0.5 5,990,766 + Pf6N2E2_5194 0.57 -0.6 5,990,766 + Pf6N2E2_5194 0.57 -1.1 5,990,766 + Pf6N2E2_5194 0.57 -0.5 5,990,996 + Pf6N2E2_5194 0.74 -0.3 5,991,138 - Pf6N2E2_5194 0.84 +2.1 5,991,349 - +1.3 5,991,349 - -1.5 5,991,426 + -0.6 5,991,426 + -1.5 5,991,427 - -1.0 5,991,427 - +0.7 5,991,451 + +1.8 5,991,452 - -0.4 5,991,470 + +0.6 5,991,670 + Pf6N2E2_5195 0.40 -1.8 5,991,671 - Pf6N2E2_5195 0.41 +0.3 5,991,728 + Pf6N2E2_5195 0.51 +1.7 5,991,743 + Pf6N2E2_5195 0.54 +1.1 5,991,767 + Pf6N2E2_5195 0.58 -1.9 5,991,767 + Pf6N2E2_5195 0.58 -1.4 5,991,767 + Pf6N2E2_5195 0.58 +1.7 5,991,768 - Pf6N2E2_5195 0.58 -1.8 5,991,768 - Pf6N2E2_5195 0.58 -0.1 5,991,889 + Pf6N2E2_5195 0.80 -0.0 5,991,890 - Pf6N2E2_5195 0.80 -1.3 5,991,932 - Pf6N2E2_5195 0.88 +0.0 5,992,033 + Pf6N2E2_5196 0.11 -2.6 5,992,033 + Pf6N2E2_5196 0.11 +0.9 5,992,033 + Pf6N2E2_5196 0.11 -0.0 5,992,033 + Pf6N2E2_5196 0.11 -0.5 5,992,033 + Pf6N2E2_5196 0.11 -0.6 5,992,033 + Pf6N2E2_5196 0.11 -0.2 5,992,033 + Pf6N2E2_5196 0.11 -1.5 5,992,033 + Pf6N2E2_5196 0.11 +1.6 5,992,033 + Pf6N2E2_5196 0.11 +0.4 5,992,033 + Pf6N2E2_5196 0.11 -0.3 5,992,033 + Pf6N2E2_5196 0.11 +1.2 5,992,033 + Pf6N2E2_5196 0.11 +1.2 5,992,034 - Pf6N2E2_5196 0.11 -1.1 5,992,034 - Pf6N2E2_5196 0.11 -0.9 5,992,034 - Pf6N2E2_5196 0.11 +0.6 5,992,034 - Pf6N2E2_5196 0.11 +0.1 5,992,034 - Pf6N2E2_5196 0.11 -0.2 5,992,034 - Pf6N2E2_5196 0.11 +0.1 5,992,034 - Pf6N2E2_5196 0.11 +0.5 5,992,034 - Pf6N2E2_5196 0.11 +1.2 5,992,082 - Pf6N2E2_5196 0.32 -0.8 5,992,133 + Pf6N2E2_5196 0.53 -1.6 5,992,133 + Pf6N2E2_5196 0.53 -1.0 5,992,133 + Pf6N2E2_5196 0.53 -0.1 5,992,134 - Pf6N2E2_5196 0.54 -0.8 5,992,134 - Pf6N2E2_5196 0.54 -0.3 5,992,134 - Pf6N2E2_5196 0.54 +1.0 5,992,134 - Pf6N2E2_5196 0.54 -0.0 5,992,135 + Pf6N2E2_5196 0.54 +0.3 5,992,135 + Pf6N2E2_5196 0.54 +0.8 5,992,135 + Pf6N2E2_5196 0.54 +0.6 5,992,135 + Pf6N2E2_5196 0.54 +0.1 5,992,136 - Pf6N2E2_5196 0.55 -0.2 5,992,136 - Pf6N2E2_5196 0.55 -1.1 5,992,136 - Pf6N2E2_5196 0.55 -0.7 5,992,136 - Pf6N2E2_5196 0.55 +0.4 5,992,167 + Pf6N2E2_5196 0.68 +0.4 5,992,167 + Pf6N2E2_5196 0.68 -1.8 5,992,168 - Pf6N2E2_5196 0.68 -1.0 5,992,168 - Pf6N2E2_5196 0.68 +0.5 5,992,168 - Pf6N2E2_5196 0.68 -1.5 5,992,168 - Pf6N2E2_5196 0.68 -0.3 5,992,200 + Pf6N2E2_5196 0.82 -1.3 5,992,201 - Pf6N2E2_5196 0.82 -1.4
Or see this region's nucleotide sequence