Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5123 and Pf6N2E2_5124 overlap by 11 nucleotides Pf6N2E2_5124 and Pf6N2E2_5125 are separated by 110 nucleotides
Pf6N2E2_5123 - Two-component sensor PilS, at 5,906,777 to 5,908,366
_5123
Pf6N2E2_5124 - FIG00953647: hypothetical protein, at 5,908,356 to 5,908,589
_5124
Pf6N2E2_5125 - Mobile element protein, at 5,908,700 to 5,910,058
_5125
Position (kb)
5908
5909 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.366 kb on - strand, within Pf6N2E2_5123 at 5907.366 kb on - strand, within Pf6N2E2_5123 at 5907.497 kb on + strand, within Pf6N2E2_5123 at 5907.519 kb on + strand, within Pf6N2E2_5123 at 5907.519 kb on + strand, within Pf6N2E2_5123 at 5907.520 kb on - strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.643 kb on - strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.675 kb on - strand, within Pf6N2E2_5123 at 5907.675 kb on - strand, within Pf6N2E2_5123 at 5907.909 kb on + strand, within Pf6N2E2_5123 at 5907.909 kb on + strand, within Pf6N2E2_5123 at 5908.018 kb on + strand, within Pf6N2E2_5123 at 5908.191 kb on + strand, within Pf6N2E2_5123 at 5908.192 kb on - strand, within Pf6N2E2_5123 at 5908.192 kb on - strand, within Pf6N2E2_5123 at 5908.405 kb on + strand, within Pf6N2E2_5124 at 5908.405 kb on + strand, within Pf6N2E2_5124 at 5908.405 kb on + strand, within Pf6N2E2_5124 at 5908.405 kb on + strand, within Pf6N2E2_5124 at 5908.406 kb on - strand, within Pf6N2E2_5124 at 5908.406 kb on - strand, within Pf6N2E2_5124 at 5908.449 kb on + strand, within Pf6N2E2_5124 at 5908.449 kb on + strand, within Pf6N2E2_5124 at 5908.449 kb on + strand, within Pf6N2E2_5124 at 5908.450 kb on - strand, within Pf6N2E2_5124 at 5908.578 kb on + strand at 5908.579 kb on - strand at 5908.579 kb on - strand at 5908.579 kb on - strand at 5908.579 kb on - strand at 5908.655 kb on + strand at 5908.668 kb on + strand at 5908.668 kb on + strand at 5908.668 kb on + strand at 5908.668 kb on + strand at 5908.668 kb on + strand at 5908.669 kb on - strand at 5908.692 kb on - strand at 5908.705 kb on + strand at 5908.705 kb on + strand at 5908.706 kb on - strand at 5908.706 kb on - strand at 5908.723 kb on + strand at 5908.724 kb on - strand at 5908.803 kb on + strand at 5908.803 kb on + strand at 5908.803 kb on + strand at 5908.804 kb on - strand at 5908.804 kb on - strand at 5908.804 kb on - strand at 5908.804 kb on - strand at 5908.804 kb on - strand at 5908.836 kb on + strand, within Pf6N2E2_5125 at 5908.836 kb on + strand, within Pf6N2E2_5125 at 5908.836 kb on + strand, within Pf6N2E2_5125 at 5908.836 kb on + strand, within Pf6N2E2_5125 at 5908.837 kb on - strand, within Pf6N2E2_5125 at 5908.837 kb on - strand, within Pf6N2E2_5125 at 5908.880 kb on - strand, within Pf6N2E2_5125 at 5909.044 kb on + strand, within Pf6N2E2_5125 at 5909.044 kb on + strand, within Pf6N2E2_5125 at 5909.044 kb on + strand, within Pf6N2E2_5125 at 5909.045 kb on - strand, within Pf6N2E2_5125 at 5909.045 kb on - strand, within Pf6N2E2_5125 at 5909.045 kb on - strand, within Pf6N2E2_5125 at 5909.045 kb on - strand, within Pf6N2E2_5125 at 5909.045 kb on - strand, within Pf6N2E2_5125 at 5909.047 kb on - strand, within Pf6N2E2_5125 at 5909.047 kb on - strand, within Pf6N2E2_5125 at 5909.113 kb on - strand, within Pf6N2E2_5125 at 5909.113 kb on - strand, within Pf6N2E2_5125 at 5909.113 kb on - strand, within Pf6N2E2_5125 at 5909.313 kb on + strand, within Pf6N2E2_5125 at 5909.387 kb on + strand, within Pf6N2E2_5125 at 5909.586 kb on + strand, within Pf6N2E2_5125
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,907,365 + Pf6N2E2_5123 0.37 -0.7 5,907,365 + Pf6N2E2_5123 0.37 -1.8 5,907,365 + Pf6N2E2_5123 0.37 +1.0 5,907,366 - Pf6N2E2_5123 0.37 +0.9 5,907,366 - Pf6N2E2_5123 0.37 +0.7 5,907,497 + Pf6N2E2_5123 0.45 +1.9 5,907,519 + Pf6N2E2_5123 0.47 -0.1 5,907,519 + Pf6N2E2_5123 0.47 -1.0 5,907,520 - Pf6N2E2_5123 0.47 +0.4 5,907,642 + Pf6N2E2_5123 0.54 +2.4 5,907,642 + Pf6N2E2_5123 0.54 -0.1 5,907,642 + Pf6N2E2_5123 0.54 -0.1 5,907,643 - Pf6N2E2_5123 0.54 +0.6 5,907,674 + Pf6N2E2_5123 0.56 +1.5 5,907,674 + Pf6N2E2_5123 0.56 -1.2 5,907,674 + Pf6N2E2_5123 0.56 +0.7 5,907,675 - Pf6N2E2_5123 0.56 -0.0 5,907,675 - Pf6N2E2_5123 0.56 +1.5 5,907,909 + Pf6N2E2_5123 0.71 -0.8 5,907,909 + Pf6N2E2_5123 0.71 +0.4 5,908,018 + Pf6N2E2_5123 0.78 -1.4 5,908,191 + Pf6N2E2_5123 0.89 -0.5 5,908,192 - Pf6N2E2_5123 0.89 +0.6 5,908,192 - Pf6N2E2_5123 0.89 -0.5 5,908,405 + Pf6N2E2_5124 0.21 -1.9 5,908,405 + Pf6N2E2_5124 0.21 -1.1 5,908,405 + Pf6N2E2_5124 0.21 -1.1 5,908,405 + Pf6N2E2_5124 0.21 -2.0 5,908,406 - Pf6N2E2_5124 0.21 -2.5 5,908,406 - Pf6N2E2_5124 0.21 -0.1 5,908,449 + Pf6N2E2_5124 0.40 +0.1 5,908,449 + Pf6N2E2_5124 0.40 -0.2 5,908,449 + Pf6N2E2_5124 0.40 +1.2 5,908,450 - Pf6N2E2_5124 0.40 +0.0 5,908,578 + +0.6 5,908,579 - -1.8 5,908,579 - +1.5 5,908,579 - +0.7 5,908,579 - +0.5 5,908,655 + -0.6 5,908,668 + +0.2 5,908,668 + -0.2 5,908,668 + -0.6 5,908,668 + +0.6 5,908,668 + -0.3 5,908,669 - -0.8 5,908,692 - +0.1 5,908,705 + -0.9 5,908,705 + +0.4 5,908,706 - -0.2 5,908,706 - +0.8 5,908,723 + +0.5 5,908,724 - +0.9 5,908,803 + -0.1 5,908,803 + +2.2 5,908,803 + -2.1 5,908,804 - +1.2 5,908,804 - -0.3 5,908,804 - -0.2 5,908,804 - -2.2 5,908,804 - -1.2 5,908,836 + Pf6N2E2_5125 0.10 +0.4 5,908,836 + Pf6N2E2_5125 0.10 -0.4 5,908,836 + Pf6N2E2_5125 0.10 +0.7 5,908,836 + Pf6N2E2_5125 0.10 +0.5 5,908,837 - Pf6N2E2_5125 0.10 -1.0 5,908,837 - Pf6N2E2_5125 0.10 +0.6 5,908,880 - Pf6N2E2_5125 0.13 +0.1 5,909,044 + Pf6N2E2_5125 0.25 -0.1 5,909,044 + Pf6N2E2_5125 0.25 +0.1 5,909,044 + Pf6N2E2_5125 0.25 -0.8 5,909,045 - Pf6N2E2_5125 0.25 +0.7 5,909,045 - Pf6N2E2_5125 0.25 +0.4 5,909,045 - Pf6N2E2_5125 0.25 +1.0 5,909,045 - Pf6N2E2_5125 0.25 +0.6 5,909,045 - Pf6N2E2_5125 0.25 +0.6 5,909,047 - Pf6N2E2_5125 0.26 -1.0 5,909,047 - Pf6N2E2_5125 0.26 -1.0 5,909,113 - Pf6N2E2_5125 0.30 +0.4 5,909,113 - Pf6N2E2_5125 0.30 +0.7 5,909,113 - Pf6N2E2_5125 0.30 -0.2 5,909,313 + Pf6N2E2_5125 0.45 -0.1 5,909,387 + Pf6N2E2_5125 0.51 +1.3 5,909,586 + Pf6N2E2_5125 0.65 +0.8
Or see this region's nucleotide sequence