Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5121 and Pf6N2E2_5122 are separated by 224 nucleotides Pf6N2E2_5122 and Pf6N2E2_5123 overlap by 4 nucleotides
Pf6N2E2_5121 - hypothetical protein, at 5,905,009 to 5,905,209
_5121
Pf6N2E2_5122 - Type IV fimbriae expression regulatory protein PilR, at 5,905,434 to 5,906,780
_5122
Pf6N2E2_5123 - Two-component sensor PilS, at 5,906,777 to 5,908,366
_5123
Position (kb)
5905
5906
5907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.443 kb on + strand at 5904.444 kb on - strand at 5904.444 kb on - strand at 5904.444 kb on - strand at 5904.444 kb on - strand at 5904.447 kb on + strand at 5904.447 kb on + strand at 5904.447 kb on + strand at 5904.448 kb on - strand at 5904.448 kb on - strand at 5904.448 kb on - strand at 5904.469 kb on + strand at 5904.469 kb on + strand at 5904.469 kb on + strand at 5904.469 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.471 kb on + strand at 5904.472 kb on - strand at 5904.472 kb on - strand at 5904.472 kb on - strand at 5904.472 kb on - strand at 5904.472 kb on - strand at 5904.678 kb on - strand at 5904.678 kb on - strand at 5904.704 kb on + strand at 5904.704 kb on + strand at 5904.705 kb on - strand at 5904.713 kb on + strand at 5904.713 kb on + strand at 5904.713 kb on + strand at 5904.713 kb on + strand at 5904.732 kb on + strand at 5904.761 kb on + strand at 5904.761 kb on + strand at 5904.761 kb on + strand at 5904.761 kb on + strand at 5904.762 kb on - strand at 5904.809 kb on + strand at 5904.810 kb on - strand at 5904.928 kb on + strand at 5904.928 kb on + strand at 5904.929 kb on - strand at 5904.929 kb on - strand at 5904.931 kb on + strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.932 kb on - strand at 5904.998 kb on + strand at 5904.998 kb on + strand at 5904.998 kb on + strand at 5904.998 kb on + strand at 5904.998 kb on + strand at 5904.999 kb on - strand at 5904.999 kb on - strand at 5904.999 kb on - strand at 5904.999 kb on - strand at 5904.999 kb on - strand at 5905.207 kb on + strand at 5905.207 kb on + strand at 5905.208 kb on - strand at 5905.252 kb on - strand at 5905.252 kb on - strand at 5905.285 kb on + strand at 5905.285 kb on + strand at 5905.289 kb on + strand at 5905.290 kb on - strand at 5905.290 kb on - strand at 5905.290 kb on - strand at 5905.290 kb on - strand at 5905.290 kb on - strand at 5905.306 kb on - strand at 5905.351 kb on + strand at 5905.570 kb on + strand, within Pf6N2E2_5122 at 5905.584 kb on - strand, within Pf6N2E2_5122 at 5905.784 kb on + strand, within Pf6N2E2_5122 at 5905.785 kb on - strand, within Pf6N2E2_5122 at 5905.785 kb on - strand, within Pf6N2E2_5122 at 5905.785 kb on - strand, within Pf6N2E2_5122 at 5905.894 kb on + strand, within Pf6N2E2_5122 at 5905.894 kb on + strand, within Pf6N2E2_5122 at 5905.894 kb on + strand, within Pf6N2E2_5122 at 5905.895 kb on - strand, within Pf6N2E2_5122 at 5905.895 kb on - strand, within Pf6N2E2_5122 at 5905.895 kb on - strand, within Pf6N2E2_5122 at 5905.895 kb on - strand, within Pf6N2E2_5122 at 5906.293 kb on + strand, within Pf6N2E2_5122 at 5906.294 kb on - strand, within Pf6N2E2_5122 at 5906.294 kb on - strand, within Pf6N2E2_5122 at 5906.294 kb on - strand, within Pf6N2E2_5122 at 5906.294 kb on - strand, within Pf6N2E2_5122 at 5906.774 kb on + strand at 5906.775 kb on - strand at 5906.846 kb on + strand at 5906.846 kb on + strand at 5906.846 kb on + strand at 5906.886 kb on + strand at 5906.886 kb on + strand at 5906.887 kb on - strand at 5906.887 kb on - strand at 5906.996 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.132 kb on + strand, within Pf6N2E2_5123 at 5907.133 kb on - strand, within Pf6N2E2_5123 at 5907.133 kb on - strand, within Pf6N2E2_5123 at 5907.313 kb on + strand, within Pf6N2E2_5123 at 5907.314 kb on - strand, within Pf6N2E2_5123 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.365 kb on + strand, within Pf6N2E2_5123 at 5907.366 kb on - strand, within Pf6N2E2_5123 at 5907.366 kb on - strand, within Pf6N2E2_5123 at 5907.497 kb on + strand, within Pf6N2E2_5123 at 5907.519 kb on + strand, within Pf6N2E2_5123 at 5907.519 kb on + strand, within Pf6N2E2_5123 at 5907.520 kb on - strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.642 kb on + strand, within Pf6N2E2_5123 at 5907.643 kb on - strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.674 kb on + strand, within Pf6N2E2_5123 at 5907.675 kb on - strand, within Pf6N2E2_5123 at 5907.675 kb on - strand, within Pf6N2E2_5123
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,904,443 + -0.0 5,904,443 + -2.1 5,904,443 + -2.3 5,904,443 + -0.3 5,904,443 + +0.0 5,904,443 + +0.2 5,904,443 + -1.1 5,904,443 + -0.0 5,904,443 + -0.0 5,904,444 - -2.6 5,904,444 - +0.7 5,904,444 - -1.1 5,904,444 - -0.6 5,904,447 + +0.0 5,904,447 + +0.6 5,904,447 + +0.1 5,904,448 - +1.2 5,904,448 - +0.4 5,904,448 - -0.7 5,904,469 + -0.8 5,904,469 + -1.4 5,904,469 + +0.3 5,904,469 + -1.4 5,904,471 + +0.5 5,904,471 + +1.7 5,904,471 + +0.4 5,904,471 + +1.0 5,904,471 + -0.2 5,904,471 + -1.0 5,904,471 + +0.1 5,904,471 + -0.8 5,904,471 + -0.7 5,904,471 + -0.8 5,904,471 + -1.5 5,904,471 + +1.4 5,904,471 + -0.3 5,904,471 + +0.7 5,904,472 - -0.1 5,904,472 - +1.9 5,904,472 - +0.3 5,904,472 - +0.4 5,904,472 - +0.6 5,904,678 - -1.1 5,904,678 - +0.2 5,904,704 + +3.3 5,904,704 + +2.7 5,904,705 - -0.1 5,904,713 + +4.3 5,904,713 + +4.1 5,904,713 + +5.1 5,904,713 + +3.5 5,904,732 + +4.0 5,904,761 + +9.4 5,904,761 + +9.0 5,904,761 + +7.0 5,904,761 + +9.4 5,904,762 - +0.8 5,904,809 + +9.4 5,904,810 - -0.1 5,904,928 + +1.2 5,904,928 + +1.2 5,904,929 - +0.1 5,904,929 - -0.3 5,904,931 + +0.3 5,904,932 - +1.0 5,904,932 - +1.0 5,904,932 - +0.4 5,904,932 - -0.3 5,904,932 - +1.7 5,904,932 - +0.5 5,904,932 - +0.7 5,904,932 - +0.9 5,904,932 - -0.6 5,904,932 - +1.0 5,904,932 - +1.0 5,904,998 + -1.5 5,904,998 + -1.2 5,904,998 + +1.0 5,904,998 + -1.0 5,904,998 + +0.3 5,904,999 - -0.6 5,904,999 - -0.8 5,904,999 - -0.2 5,904,999 - +1.5 5,904,999 - -0.3 5,905,207 + -1.3 5,905,207 + +0.7 5,905,208 - +0.5 5,905,252 - -2.4 5,905,252 - -3.0 5,905,285 + +1.0 5,905,285 + -0.2 5,905,289 + -0.3 5,905,290 - -0.8 5,905,290 - +1.8 5,905,290 - -1.3 5,905,290 - +0.7 5,905,290 - -0.6 5,905,306 - -1.2 5,905,351 + -0.9 5,905,570 + Pf6N2E2_5122 0.10 -0.2 5,905,584 - Pf6N2E2_5122 0.11 -1.4 5,905,784 + Pf6N2E2_5122 0.26 +1.1 5,905,785 - Pf6N2E2_5122 0.26 -1.7 5,905,785 - Pf6N2E2_5122 0.26 +0.0 5,905,785 - Pf6N2E2_5122 0.26 -1.9 5,905,894 + Pf6N2E2_5122 0.34 +2.0 5,905,894 + Pf6N2E2_5122 0.34 +2.8 5,905,894 + Pf6N2E2_5122 0.34 +0.2 5,905,895 - Pf6N2E2_5122 0.34 -1.7 5,905,895 - Pf6N2E2_5122 0.34 -2.0 5,905,895 - Pf6N2E2_5122 0.34 -0.2 5,905,895 - Pf6N2E2_5122 0.34 +0.8 5,906,293 + Pf6N2E2_5122 0.64 +0.4 5,906,294 - Pf6N2E2_5122 0.64 +1.0 5,906,294 - Pf6N2E2_5122 0.64 +1.5 5,906,294 - Pf6N2E2_5122 0.64 -1.2 5,906,294 - Pf6N2E2_5122 0.64 -1.4 5,906,774 + +2.9 5,906,775 - -0.2 5,906,846 + +2.6 5,906,846 + +0.8 5,906,846 + -0.9 5,906,886 + -1.3 5,906,886 + +0.5 5,906,887 - -0.1 5,906,887 - -0.5 5,906,996 + Pf6N2E2_5123 0.14 -0.9 5,907,132 + Pf6N2E2_5123 0.22 -1.5 5,907,132 + Pf6N2E2_5123 0.22 -2.3 5,907,132 + Pf6N2E2_5123 0.22 +0.7 5,907,132 + Pf6N2E2_5123 0.22 -0.5 5,907,132 + Pf6N2E2_5123 0.22 -0.9 5,907,132 + Pf6N2E2_5123 0.22 +2.0 5,907,133 - Pf6N2E2_5123 0.22 +1.3 5,907,133 - Pf6N2E2_5123 0.22 -0.2 5,907,313 + Pf6N2E2_5123 0.34 +1.2 5,907,314 - Pf6N2E2_5123 0.34 +1.5 5,907,365 + Pf6N2E2_5123 0.37 -0.7 5,907,365 + Pf6N2E2_5123 0.37 -1.8 5,907,365 + Pf6N2E2_5123 0.37 +1.0 5,907,366 - Pf6N2E2_5123 0.37 +0.9 5,907,366 - Pf6N2E2_5123 0.37 +0.7 5,907,497 + Pf6N2E2_5123 0.45 +1.9 5,907,519 + Pf6N2E2_5123 0.47 -0.1 5,907,519 + Pf6N2E2_5123 0.47 -1.0 5,907,520 - Pf6N2E2_5123 0.47 +0.4 5,907,642 + Pf6N2E2_5123 0.54 +2.4 5,907,642 + Pf6N2E2_5123 0.54 -0.1 5,907,642 + Pf6N2E2_5123 0.54 -0.1 5,907,643 - Pf6N2E2_5123 0.54 +0.6 5,907,674 + Pf6N2E2_5123 0.56 +1.5 5,907,674 + Pf6N2E2_5123 0.56 -1.2 5,907,674 + Pf6N2E2_5123 0.56 +0.7 5,907,675 - Pf6N2E2_5123 0.56 -0.0 5,907,675 - Pf6N2E2_5123 0.56 +1.5
Or see this region's nucleotide sequence