Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4665

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
5,393,613	+				-0.5
5,393,614	-				+0.2
5,393,634	+		Pf6N2E2_4664	0.12	+0.8
5,393,634	+		Pf6N2E2_4664	0.12	-0.2
5,393,634	+		Pf6N2E2_4664	0.12	+0.1
5,393,634	+		Pf6N2E2_4664	0.12	+0.8
5,393,634	+		Pf6N2E2_4664	0.12	-0.2
5,393,634	+		Pf6N2E2_4664	0.12	-0.2
5,393,634	+		Pf6N2E2_4664	0.12	-1.7
5,393,634	+		Pf6N2E2_4664	0.12	-2.6
5,393,634	+		Pf6N2E2_4664	0.12	-0.6
5,393,635	-		Pf6N2E2_4664	0.12	+0.8
5,393,635	-		Pf6N2E2_4664	0.12	-0.2
5,393,635	-		Pf6N2E2_4664	0.12	-0.6
5,393,635	-		Pf6N2E2_4664	0.12	+0.5
5,393,674	+		Pf6N2E2_4664	0.18	-2.1
5,393,675	-		Pf6N2E2_4664	0.18	+0.0
5,393,700	+		Pf6N2E2_4664	0.22	+1.1
5,393,700	+		Pf6N2E2_4664	0.22	-1.2
5,393,751	+		Pf6N2E2_4664	0.29	-0.3
5,393,751	+		Pf6N2E2_4664	0.29	+0.0
5,393,751	+		Pf6N2E2_4664	0.29	-1.4
5,393,752	-		Pf6N2E2_4664	0.29	+0.1
5,393,752	-		Pf6N2E2_4664	0.29	-0.9
5,393,752	-		Pf6N2E2_4664	0.29	-1.5
5,393,791	+		Pf6N2E2_4664	0.35	-0.7
5,393,791	+		Pf6N2E2_4664	0.35	+1.2
5,393,792	-		Pf6N2E2_4664	0.35	+0.2
5,393,792	-		Pf6N2E2_4664	0.35	-0.4
5,393,859	+		Pf6N2E2_4664	0.45	-1.7
5,393,860	-		Pf6N2E2_4664	0.46	+0.0
5,393,860	-		Pf6N2E2_4664	0.46	-1.3
5,393,860	-		Pf6N2E2_4664	0.46	+0.9
5,393,860	-		Pf6N2E2_4664	0.46	+1.3
5,393,860	-		Pf6N2E2_4664	0.46	-1.8
5,393,869	-		Pf6N2E2_4664	0.47	-0.3
5,393,875	-		Pf6N2E2_4664	0.48	+0.0
5,393,949	-		Pf6N2E2_4664	0.59	+2.1
5,394,028	-		Pf6N2E2_4664	0.71	-0.8
5,394,055	-		Pf6N2E2_4664	0.75	-0.5
5,394,126	+		Pf6N2E2_4664	0.85	-0.8
5,394,126	+		Pf6N2E2_4664	0.85	+0.0
5,394,126	+		Pf6N2E2_4664	0.85	+0.2
5,394,126	+		Pf6N2E2_4664	0.85	-1.2
5,394,126	+		Pf6N2E2_4664	0.85	-1.4
5,394,126	+		Pf6N2E2_4664	0.85	-1.0
5,394,127	-		Pf6N2E2_4664	0.86	-0.5
5,394,127	-		Pf6N2E2_4664	0.86	-0.4
5,394,127	-		Pf6N2E2_4664	0.86	+0.7
5,394,127	-		Pf6N2E2_4664	0.86	+0.8
5,394,127	-		Pf6N2E2_4664	0.86	-1.1
5,394,247	+				+0.0
5,394,248	-				-0.1
5,394,260	+				+0.8
5,394,260	+				-0.1
5,394,261	-				+0.7
5,394,278	+				-1.5
5,394,279	-				-0.8
5,394,387	+				+0.9
5,394,387	+				+0.4
5,394,387	+				-0.3
5,394,387	+				-1.5
5,394,387	+				-0.3
5,394,387	+				+0.1
5,394,387	+				-0.4
5,394,387	+				-1.3
5,394,387	+				-0.7
5,394,387	+				+1.5
5,394,387	+				-0.1
5,394,388	-				-1.2
5,394,388	-				+0.7
5,394,388	-				-0.9
5,394,388	-				-1.2
5,394,388	-				+0.8
5,394,388	-				+0.0
5,394,388	-				-0.5
5,394,388	-				+0.8
5,394,388	-				+0.2
5,394,437	+				+1.0
5,394,437	+				+0.3
5,394,455	+				+0.9
5,394,455	+				-1.2
5,394,507	+				-0.6
5,394,507	+				-1.1
5,394,508	-				+0.2
5,394,508	-				+1.5
5,394,508	-				-0.7
5,394,582	+		Pf6N2E2_4665	0.11	+3.6
5,394,582	+		Pf6N2E2_4665	0.11	-3.3
5,394,583	-		Pf6N2E2_4665	0.12	+2.1
5,394,583	-		Pf6N2E2_4665	0.12	+0.4
5,394,583	-		Pf6N2E2_4665	0.12	+0.2
5,394,600	+		Pf6N2E2_4665	0.14	-1.8
5,394,601	-		Pf6N2E2_4665	0.14	-0.4
5,394,699	+		Pf6N2E2_4665	0.29	-0.9
5,394,700	-		Pf6N2E2_4665	0.29	+0.4
5,394,700	-		Pf6N2E2_4665	0.29	-0.8
5,394,700	-		Pf6N2E2_4665	0.29	+1.5
5,394,700	-		Pf6N2E2_4665	0.29	-1.0
5,394,700	-		Pf6N2E2_4665	0.29	+0.9
5,394,700	-		Pf6N2E2_4665	0.29	+0.2
5,394,814	+		Pf6N2E2_4665	0.46	+1.2
5,394,814	+		Pf6N2E2_4665	0.46	-1.3
5,394,814	+		Pf6N2E2_4665	0.46	-0.3
5,394,814	+		Pf6N2E2_4665	0.46	-0.8
5,394,814	+		Pf6N2E2_4665	0.46	-1.3
5,394,815	-		Pf6N2E2_4665	0.46	-0.2
5,394,815	-		Pf6N2E2_4665	0.46	+1.9
5,394,815	-		Pf6N2E2_4665	0.46	-1.9
5,394,815	-		Pf6N2E2_4665	0.46	+0.1
5,394,815	-		Pf6N2E2_4665	0.46	+0.4
5,394,815	-		Pf6N2E2_4665	0.46	+0.4
5,394,815	-		Pf6N2E2_4665	0.46	-1.6
5,394,815	-		Pf6N2E2_4665	0.46	+0.7
5,394,816	+		Pf6N2E2_4665	0.47	+1.2
5,394,816	+		Pf6N2E2_4665	0.47	+0.3
5,394,817	-		Pf6N2E2_4665	0.47	+0.5
5,394,817	-		Pf6N2E2_4665	0.47	+1.9
5,394,860	-		Pf6N2E2_4665	0.53	-1.1
5,394,929	-		Pf6N2E2_4665	0.64	+0.8
5,394,975	+		Pf6N2E2_4665	0.70	+1.8
5,394,975	+		Pf6N2E2_4665	0.70	-2.6
5,394,976	-		Pf6N2E2_4665	0.71	-0.9
5,394,976	-		Pf6N2E2_4665	0.71	-1.5
5,395,002	+		Pf6N2E2_4665	0.74	-1.1
5,395,002	+		Pf6N2E2_4665	0.74	-0.8
5,395,002	+		Pf6N2E2_4665	0.74	-1.0
5,395,006	+		Pf6N2E2_4665	0.75	-0.8
5,395,006	+		Pf6N2E2_4665	0.75	-1.0
5,395,006	+		Pf6N2E2_4665	0.75	-1.8
5,395,006	+		Pf6N2E2_4665	0.75	-0.9
5,395,006	+		Pf6N2E2_4665	0.75	+0.9
5,395,006	+		Pf6N2E2_4665	0.75	-2.1
5,395,006	+		Pf6N2E2_4665	0.75	-0.2
5,395,006	+		Pf6N2E2_4665	0.75	+1.5
5,395,006	+		Pf6N2E2_4665	0.75	-1.8
5,395,007	-		Pf6N2E2_4665	0.75	+0.1
5,395,007	-		Pf6N2E2_4665	0.75	+0.3
5,395,007	-		Pf6N2E2_4665	0.75	+0.9
5,395,074	+		Pf6N2E2_4665	0.85	-1.2
5,395,117	+				-2.4
5,395,182	+				-1.3
5,395,182	+				+0.2
5,395,182	+				-1.3
5,395,182	+				-1.6
5,395,182	+				-1.2
5,395,182	+				-1.3
5,395,182	+				+0.6
5,395,182	+				-0.8
5,395,182	+				+1.8
5,395,183	-				-0.4
5,395,183	-				+0.6
5,395,183	-				+0.8
5,395,183	-				-1.5
5,395,183	-				-0.7
5,395,183	-				+1.9
5,395,183	-				+1.3
5,395,183	-				+0.1
5,395,225	+				+1.3
5,395,225	+				+0.1
5,395,246	-				+1.1
5,395,401	-				-0.2
5,395,423	+				-1.2
5,395,423	+				+1.6
5,395,423	+				-2.1
5,395,424	-				-0.2
5,395,439	+				-0.5
5,395,439	+				-1.3
5,395,439	+				-0.6
5,395,439	+				-0.3
5,395,439	+				-1.0
5,395,439	+				-2.3
5,395,439	+				-1.6
5,395,440	-				-0.2
5,395,440	-				+0.0
5,395,440	-				+0.6
5,395,440	-				-0.6
5,395,440	-				-0.3
5,395,440	-				+0.2
5,395,440	-				+0.7
5,395,440	-				+0.2
5,395,455	+				+0.2
5,395,455	+				+0.3
5,395,456	-				-0.3
5,395,456	-				-0.2
5,395,459	+				-2.2
5,395,641	+				+0.4
5,395,743	+		Pf6N2E2_4666	0.12	+1.9
5,395,811	-		Pf6N2E2_4666	0.15	+0.3
5,395,811	-		Pf6N2E2_4666	0.15	+0.0
5,395,831	+		Pf6N2E2_4666	0.16	-1.7
5,395,831	+		Pf6N2E2_4666	0.16	+1.3
5,395,831	+		Pf6N2E2_4666	0.16	-0.7
5,395,953	+		Pf6N2E2_4666	0.22	+1.5
5,396,034	+		Pf6N2E2_4666	0.25	+0.4
5,396,034	+		Pf6N2E2_4666	0.25	-0.8
5,396,069	+		Pf6N2E2_4666	0.27	+0.9
5,396,069	+		Pf6N2E2_4666	0.27	-1.1
5,396,069	+		Pf6N2E2_4666	0.27	-0.5
5,396,069	+		Pf6N2E2_4666	0.27	+0.6
5,396,070	-		Pf6N2E2_4666	0.27	+1.1
5,396,070	-		Pf6N2E2_4666	0.27	+0.2
5,396,070	-		Pf6N2E2_4666	0.27	+0.2
5,396,070	-		Pf6N2E2_4666	0.27	+0.8