Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4616 and Pf6N2E2_4617 are separated by 185 nucleotides Pf6N2E2_4617 and Pf6N2E2_4618 overlap by 8 nucleotides
Pf6N2E2_4616 - type IV pilus biogenesis protein PilJ, at 5,339,627 to 5,341,690
_4616
Pf6N2E2_4617 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 5,341,876 to 5,347,809
_4617
Pf6N2E2_4618 - Chemotaxis signal transduction protein, at 5,347,802 to 5,348,281
_4618
Position (kb) 5341
5342
5343
5344
5345
5346
5347
5348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5340.944 kb on - strand, within Pf6N2E2_4616 at 5340.944 kb on - strand, within Pf6N2E2_4616 at 5341.108 kb on + strand, within Pf6N2E2_4616 at 5341.108 kb on + strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.109 kb on - strand, within Pf6N2E2_4616 at 5341.645 kb on + strand at 5341.646 kb on - strand at 5341.646 kb on - strand at 5341.712 kb on + strand at 5341.712 kb on + strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.713 kb on - strand at 5341.780 kb on - strand at 5341.841 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.852 kb on + strand at 5341.853 kb on - strand at 5341.853 kb on - strand at 5341.853 kb on - strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.894 kb on + strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5341.895 kb on - strand at 5342.056 kb on + strand at 5342.056 kb on + strand at 5342.057 kb on - strand at 5342.179 kb on + strand at 5342.179 kb on + strand at 5342.180 kb on - strand at 5342.180 kb on - strand at 5342.180 kb on - strand at 5342.192 kb on + strand at 5342.193 kb on - strand at 5342.193 kb on - strand at 5342.193 kb on - strand at 5342.450 kb on - strand at 5342.510 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.686 kb on + strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.687 kb on - strand, within Pf6N2E2_4617 at 5342.688 kb on + strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.689 kb on - strand, within Pf6N2E2_4617 at 5342.740 kb on + strand, within Pf6N2E2_4617 at 5342.858 kb on + strand, within Pf6N2E2_4617 at 5343.088 kb on + strand, within Pf6N2E2_4617 at 5343.089 kb on - strand, within Pf6N2E2_4617 at 5343.244 kb on + strand, within Pf6N2E2_4617 at 5343.245 kb on - strand, within Pf6N2E2_4617 at 5343.457 kb on + strand, within Pf6N2E2_4617 at 5343.457 kb on + strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.458 kb on - strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.460 kb on + strand, within Pf6N2E2_4617 at 5343.461 kb on - strand, within Pf6N2E2_4617 at 5343.461 kb on - strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.817 kb on + strand, within Pf6N2E2_4617 at 5343.818 kb on - strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.299 kb on + strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.300 kb on - strand, within Pf6N2E2_4617 at 5344.798 kb on + strand, within Pf6N2E2_4617 at 5344.798 kb on + strand, within Pf6N2E2_4617 at 5344.799 kb on - strand, within Pf6N2E2_4617 at 5344.934 kb on + strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5344.935 kb on - strand, within Pf6N2E2_4617 at 5345.288 kb on - strand, within Pf6N2E2_4617 at 5345.288 kb on - strand at 5345.317 kb on + strand, within Pf6N2E2_4617 at 5345.318 kb on - strand, within Pf6N2E2_4617 at 5345.318 kb on - strand, within Pf6N2E2_4617 at 5345.556 kb on + strand, within Pf6N2E2_4617 at 5345.557 kb on - strand, within Pf6N2E2_4617 at 5345.557 kb on - strand, within Pf6N2E2_4617 at 5345.624 kb on + strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.711 kb on - strand, within Pf6N2E2_4617 at 5345.922 kb on + strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5345.923 kb on - strand, within Pf6N2E2_4617 at 5346.370 kb on - strand, within Pf6N2E2_4617 at 5346.526 kb on - strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.561 kb on + strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.562 kb on - strand, within Pf6N2E2_4617 at 5346.639 kb on + strand, within Pf6N2E2_4617 at 5346.640 kb on - strand, within Pf6N2E2_4617 at 5346.640 kb on - strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.093 kb on + strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.094 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.100 kb on - strand, within Pf6N2E2_4617 at 5347.127 kb on - strand, within Pf6N2E2_4617 at 5347.413 kb on - strand at 5347.413 kb on - strand at 5347.738 kb on + strand at 5347.739 kb on - strand at 5347.753 kb on + strand at 5348.123 kb on + strand, within Pf6N2E2_4618 at 5348.124 kb on - strand, within Pf6N2E2_4618 at 5348.148 kb on - strand, within Pf6N2E2_4618 at 5348.148 kb on - strand, within Pf6N2E2_4618 at 5348.205 kb on + strand, within Pf6N2E2_4618 at 5348.205 kb on + strand, within Pf6N2E2_4618 at 5348.206 kb on - strand, within Pf6N2E2_4618 at 5348.206 kb on - strand, within Pf6N2E2_4618 at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.286 kb on + strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.287 kb on - strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.296 kb on + strand at 5348.297 kb on - strand at 5348.370 kb on - strand at 5348.370 kb on - strand at 5348.414 kb on + strand at 5348.564 kb on - strand at 5348.626 kb on + strand at 5348.626 kb on + strand at 5348.628 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.655 kb on + strand at 5348.656 kb on - strand at 5348.656 kb on - strand at 5348.656 kb on - strand at 5348.717 kb on + strand at 5348.719 kb on + strand at 5348.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,340,944 - Pf6N2E2_4616 0.64 +3.7 5,340,944 - Pf6N2E2_4616 0.64 +3.0 5,341,108 + Pf6N2E2_4616 0.72 +3.2 5,341,108 + Pf6N2E2_4616 0.72 +2.7 5,341,109 - Pf6N2E2_4616 0.72 +2.9 5,341,109 - Pf6N2E2_4616 0.72 +2.1 5,341,109 - Pf6N2E2_4616 0.72 +2.4 5,341,645 + +2.0 5,341,646 - +3.2 5,341,646 - +2.7 5,341,712 + +0.1 5,341,712 + -0.8 5,341,713 - +0.6 5,341,713 - -0.2 5,341,713 - -2.5 5,341,713 - -0.5 5,341,713 - -1.4 5,341,713 - -1.7 5,341,713 - -2.2 5,341,780 - +0.3 5,341,841 + +1.6 5,341,852 + +0.6 5,341,852 + +2.4 5,341,852 + +1.1 5,341,852 + +3.7 5,341,852 + +1.8 5,341,852 + -0.1 5,341,852 + +1.8 5,341,853 - +0.3 5,341,853 - +0.0 5,341,853 - +0.2 5,341,894 + +1.1 5,341,894 + +2.0 5,341,894 + -1.0 5,341,894 + +0.6 5,341,894 + +0.5 5,341,894 + +1.9 5,341,894 + +1.3 5,341,895 - -1.7 5,341,895 - -1.8 5,341,895 - +0.5 5,341,895 - -2.9 5,341,895 - +0.2 5,341,895 - -0.3 5,342,056 + +1.7 5,342,056 + -0.1 5,342,057 - +1.3 5,342,179 + +1.6 5,342,179 + +3.9 5,342,180 - +0.3 5,342,180 - -0.8 5,342,180 - -0.3 5,342,192 + +0.6 5,342,193 - -1.4 5,342,193 - -0.7 5,342,193 - +0.1 5,342,450 - +0.2 5,342,510 + Pf6N2E2_4617 0.11 +1.1 5,342,686 + Pf6N2E2_4617 0.14 +1.5 5,342,686 + Pf6N2E2_4617 0.14 +1.9 5,342,686 + Pf6N2E2_4617 0.14 +0.7 5,342,686 + Pf6N2E2_4617 0.14 -0.3 5,342,686 + Pf6N2E2_4617 0.14 +2.7 5,342,686 + Pf6N2E2_4617 0.14 +3.2 5,342,686 + Pf6N2E2_4617 0.14 +0.5 5,342,686 + Pf6N2E2_4617 0.14 +1.7 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,687 - Pf6N2E2_4617 0.14 +1.1 5,342,687 - Pf6N2E2_4617 0.14 -1.2 5,342,687 - Pf6N2E2_4617 0.14 -1.4 5,342,687 - Pf6N2E2_4617 0.14 -0.5 5,342,687 - Pf6N2E2_4617 0.14 -1.4 5,342,687 - Pf6N2E2_4617 0.14 -2.0 5,342,687 - Pf6N2E2_4617 0.14 -0.5 5,342,687 - Pf6N2E2_4617 0.14 -0.1 5,342,687 - Pf6N2E2_4617 0.14 -0.3 5,342,688 + Pf6N2E2_4617 0.14 +1.9 5,342,689 - Pf6N2E2_4617 0.14 -0.6 5,342,689 - Pf6N2E2_4617 0.14 +0.0 5,342,689 - Pf6N2E2_4617 0.14 -0.7 5,342,740 + Pf6N2E2_4617 0.15 -1.8 5,342,858 + Pf6N2E2_4617 0.17 -1.1 5,343,088 + Pf6N2E2_4617 0.20 -0.8 5,343,089 - Pf6N2E2_4617 0.20 +2.7 5,343,244 + Pf6N2E2_4617 0.23 -2.0 5,343,245 - Pf6N2E2_4617 0.23 +2.6 5,343,457 + Pf6N2E2_4617 0.27 -2.7 5,343,457 + Pf6N2E2_4617 0.27 -2.7 5,343,458 - Pf6N2E2_4617 0.27 +4.4 5,343,458 - Pf6N2E2_4617 0.27 +3.7 5,343,458 - Pf6N2E2_4617 0.27 +0.9 5,343,458 - Pf6N2E2_4617 0.27 +2.4 5,343,458 - Pf6N2E2_4617 0.27 +3.8 5,343,460 + Pf6N2E2_4617 0.27 -1.1 5,343,460 + Pf6N2E2_4617 0.27 +1.1 5,343,460 + Pf6N2E2_4617 0.27 -3.1 5,343,460 + Pf6N2E2_4617 0.27 -0.9 5,343,461 - Pf6N2E2_4617 0.27 +2.8 5,343,461 - Pf6N2E2_4617 0.27 +4.2 5,343,817 + Pf6N2E2_4617 0.33 -1.1 5,343,817 + Pf6N2E2_4617 0.33 +0.1 5,343,817 + Pf6N2E2_4617 0.33 -0.5 5,343,818 - Pf6N2E2_4617 0.33 +3.4 5,344,299 + Pf6N2E2_4617 0.41 +0.7 5,344,299 + Pf6N2E2_4617 0.41 -1.2 5,344,299 + Pf6N2E2_4617 0.41 -2.2 5,344,299 + Pf6N2E2_4617 0.41 -0.4 5,344,299 + Pf6N2E2_4617 0.41 -1.5 5,344,299 + Pf6N2E2_4617 0.41 -2.1 5,344,300 - Pf6N2E2_4617 0.41 +1.7 5,344,300 - Pf6N2E2_4617 0.41 +3.0 5,344,300 - Pf6N2E2_4617 0.41 +2.0 5,344,798 + Pf6N2E2_4617 0.49 -0.7 5,344,798 + Pf6N2E2_4617 0.49 +0.0 5,344,799 - Pf6N2E2_4617 0.49 +0.9 5,344,934 + Pf6N2E2_4617 0.52 -1.8 5,344,935 - Pf6N2E2_4617 0.52 -0.7 5,344,935 - Pf6N2E2_4617 0.52 +1.4 5,344,935 - Pf6N2E2_4617 0.52 +4.1 5,345,288 - Pf6N2E2_4617 0.57 +4.5 5,345,288 - +4.7 5,345,317 + Pf6N2E2_4617 0.58 +3.7 5,345,318 - Pf6N2E2_4617 0.58 +2.7 5,345,318 - Pf6N2E2_4617 0.58 +2.9 5,345,556 + Pf6N2E2_4617 0.62 +1.2 5,345,557 - Pf6N2E2_4617 0.62 +4.8 5,345,557 - Pf6N2E2_4617 0.62 +3.4 5,345,624 + Pf6N2E2_4617 0.63 +1.7 5,345,711 - Pf6N2E2_4617 0.65 +2.7 5,345,711 - Pf6N2E2_4617 0.65 +1.0 5,345,711 - Pf6N2E2_4617 0.65 +0.8 5,345,711 - Pf6N2E2_4617 0.65 +0.3 5,345,711 - Pf6N2E2_4617 0.65 +1.9 5,345,922 + Pf6N2E2_4617 0.68 +2.8 5,345,923 - Pf6N2E2_4617 0.68 +2.8 5,345,923 - Pf6N2E2_4617 0.68 +2.1 5,345,923 - Pf6N2E2_4617 0.68 +0.5 5,346,370 - Pf6N2E2_4617 0.76 +0.9 5,346,526 - Pf6N2E2_4617 0.78 +3.1 5,346,561 + Pf6N2E2_4617 0.79 +2.1 5,346,561 + Pf6N2E2_4617 0.79 +2.4 5,346,561 + Pf6N2E2_4617 0.79 +2.9 5,346,561 + +3.9 5,346,561 + Pf6N2E2_4617 0.79 +2.4 5,346,561 + Pf6N2E2_4617 0.79 +2.6 5,346,561 + Pf6N2E2_4617 0.79 +3.5 5,346,562 - Pf6N2E2_4617 0.79 +2.4 5,346,562 - Pf6N2E2_4617 0.79 +0.8 5,346,562 - Pf6N2E2_4617 0.79 +3.1 5,346,562 - Pf6N2E2_4617 0.79 +4.0 5,346,639 + Pf6N2E2_4617 0.80 +3.1 5,346,640 - Pf6N2E2_4617 0.80 +1.9 5,346,640 - Pf6N2E2_4617 0.80 +0.3 5,347,093 + Pf6N2E2_4617 0.88 +3.1 5,347,093 + Pf6N2E2_4617 0.88 +4.5 5,347,093 + Pf6N2E2_4617 0.88 +2.1 5,347,093 + Pf6N2E2_4617 0.88 +2.6 5,347,093 + Pf6N2E2_4617 0.88 +0.9 5,347,093 + Pf6N2E2_4617 0.88 +2.3 5,347,093 + Pf6N2E2_4617 0.88 +3.9 5,347,093 + Pf6N2E2_4617 0.88 +2.9 5,347,093 + Pf6N2E2_4617 0.88 +3.0 5,347,093 + Pf6N2E2_4617 0.88 +3.0 5,347,093 + Pf6N2E2_4617 0.88 +3.5 5,347,093 + Pf6N2E2_4617 0.88 +2.0 5,347,094 - Pf6N2E2_4617 0.88 +3.8 5,347,094 - Pf6N2E2_4617 0.88 +3.0 5,347,094 - Pf6N2E2_4617 0.88 +1.9 5,347,094 - Pf6N2E2_4617 0.88 +0.4 5,347,094 - Pf6N2E2_4617 0.88 +4.2 5,347,094 - Pf6N2E2_4617 0.88 +2.7 5,347,094 - Pf6N2E2_4617 0.88 +3.4 5,347,100 - Pf6N2E2_4617 0.88 +3.5 5,347,100 - Pf6N2E2_4617 0.88 +3.6 5,347,100 - Pf6N2E2_4617 0.88 +3.9 5,347,127 - Pf6N2E2_4617 0.88 +4.5 5,347,413 - +1.5 5,347,413 - +2.6 5,347,738 + +0.9 5,347,739 - +2.8 5,347,753 + -2.3 5,348,123 + Pf6N2E2_4618 0.67 +1.9 5,348,124 - Pf6N2E2_4618 0.67 +2.2 5,348,148 - Pf6N2E2_4618 0.72 +5.4 5,348,148 - Pf6N2E2_4618 0.72 +3.7 5,348,205 + Pf6N2E2_4618 0.84 +3.8 5,348,205 + Pf6N2E2_4618 0.84 +0.3 5,348,206 - Pf6N2E2_4618 0.84 +4.1 5,348,206 - Pf6N2E2_4618 0.84 +2.7 5,348,286 + +1.8 5,348,286 + +0.4 5,348,286 + +0.3 5,348,286 + +0.5 5,348,286 + +1.1 5,348,286 + -0.1 5,348,286 + +0.2 5,348,286 + +0.6 5,348,287 - +0.4 5,348,287 - +1.0 5,348,287 - +0.9 5,348,287 - -0.0 5,348,287 - +0.5 5,348,296 + +0.0 5,348,296 + -0.6 5,348,296 + +0.2 5,348,296 + -0.2 5,348,297 - -0.3 5,348,370 - +0.1 5,348,370 - +1.1 5,348,414 + +1.5 5,348,564 - -0.5 5,348,626 + -0.5 5,348,626 + +0.5 5,348,628 + -0.3 5,348,655 + -0.6 5,348,655 + -0.2 5,348,655 + -1.6 5,348,655 + -0.4 5,348,655 + +1.9 5,348,656 - +1.0 5,348,656 - -0.4 5,348,656 - -0.2 5,348,717 + +1.5 5,348,719 + -1.8 5,348,720 - +0.6
Or see this region's nucleotide sequence