Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4259

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
4,956,458	+		Pf6N2E2_4257	0.86	+0.7
4,956,458	+		Pf6N2E2_4257	0.86	-1.0
4,956,458	+		Pf6N2E2_4257	0.86	+1.2
4,956,458	+		Pf6N2E2_4257	0.86	+1.2
4,956,458	+		Pf6N2E2_4257	0.86	-1.0
4,956,458	+		Pf6N2E2_4257	0.86	-0.6
4,956,458	+		Pf6N2E2_4257	0.86	+1.3
4,956,458	+		Pf6N2E2_4257	0.86	-0.7
4,956,459	-		Pf6N2E2_4257	0.86	-0.8
4,956,459	-		Pf6N2E2_4257	0.86	+1.1
4,956,459	-		Pf6N2E2_4257	0.86	-1.0
4,956,459	-		Pf6N2E2_4257	0.86	+1.0
4,956,459	-		Pf6N2E2_4257	0.86	+2.0
4,956,459	-		Pf6N2E2_4257	0.86	-1.2
4,956,459	-		Pf6N2E2_4257	0.86	-0.0
4,956,459	-		Pf6N2E2_4257	0.86	+0.8
4,956,459	-		Pf6N2E2_4257	0.86	+0.7
4,956,491	+		Pf6N2E2_4257	0.89	+0.7
4,956,510	-		Pf6N2E2_4257	0.90	+0.9
4,956,516	+				-0.8
4,956,516	+				-2.7
4,956,516	+				+0.6
4,956,516	+				+0.3
4,956,517	-				-0.4
4,956,517	-				-2.0
4,956,517	-				-2.1
4,956,517	-				-1.7
4,956,517	-				+0.6
4,956,517	-				+0.4
4,956,647	+				+0.3
4,956,686	+				+0.5
4,956,686	+				-0.8
4,956,686	+				-2.7
4,956,686	+				+0.0
4,956,716	+				-0.9
4,956,720	+				+2.0
4,956,720	+				+1.7
4,956,720	+				+1.7
4,956,721	-				-0.0
4,956,721	-				+1.8
4,956,721	-				-0.7
4,956,721	-				+0.9
4,956,721	-				-0.1
4,956,794	+				+1.5
4,956,795	-				+0.8
4,956,795	-				+0.2
4,956,795	-				+0.8
4,956,903	+		Pf6N2E2_4258	0.20	-1.8
4,956,903	+		Pf6N2E2_4258	0.20	+0.4
4,956,903	+		Pf6N2E2_4258	0.20	-1.4
4,956,903	+		Pf6N2E2_4258	0.20	-0.1
4,956,903	+		Pf6N2E2_4258	0.20	+0.7
4,956,903	+		Pf6N2E2_4258	0.20	+0.5
4,956,903	+		Pf6N2E2_4258	0.20	-1.9
4,956,904	-		Pf6N2E2_4258	0.21	+0.6
4,956,904	-		Pf6N2E2_4258	0.21	+1.4
4,956,904	-		Pf6N2E2_4258	0.21	+1.3
4,956,904	-		Pf6N2E2_4258	0.21	-2.0
4,956,904	-		Pf6N2E2_4258	0.21	+0.3
4,956,904	-		Pf6N2E2_4258	0.21	-1.6
4,957,008	+		Pf6N2E2_4258	0.41	+0.1
4,957,008	+		Pf6N2E2_4258	0.41	-0.4
4,957,008	+		Pf6N2E2_4258	0.41	+0.6
4,957,009	-		Pf6N2E2_4258	0.41	+0.2
4,957,009	-		Pf6N2E2_4258	0.41	-0.1
4,957,009	-		Pf6N2E2_4258	0.41	-1.1
4,957,167	+		Pf6N2E2_4258	0.71	-1.4
4,957,331	+				-0.3
4,957,332	-				+0.8
4,957,332	-				-0.9
4,957,443	+				+0.1
4,957,444	-				-0.1
4,957,444	-				+0.7
4,957,444	-				-1.3
4,957,464	+				+1.2
4,957,464	+				+0.1
4,957,464	+				-1.1
4,957,465	-				-2.0
4,957,471	+				+0.3
4,957,471	+				+0.9
4,957,471	+				-0.1
4,957,471	+				+0.2
4,957,471	+				-1.1
4,957,471	+				+0.6
4,957,472	-				+0.4
4,957,472	-				-0.3
4,957,482	+				-1.5
4,957,483	-				-1.9
4,957,483	-				-1.4
4,957,483	-				-0.3
4,957,483	-				-0.1
4,957,539	+		Pf6N2E2_4259	0.11	-2.3
4,957,539	+		Pf6N2E2_4259	0.11	+0.5
4,957,728	+		Pf6N2E2_4259	0.33	-1.3
4,957,729	-		Pf6N2E2_4259	0.33	+0.4
4,957,729	-		Pf6N2E2_4259	0.33	-2.6
4,957,729	-		Pf6N2E2_4259	0.33	+0.2
4,957,729	-		Pf6N2E2_4259	0.33	-1.0
4,957,830	+		Pf6N2E2_4259	0.44	+0.2
4,957,830	+		Pf6N2E2_4259	0.44	-1.9
4,957,831	-		Pf6N2E2_4259	0.44	+0.4
4,957,845	+		Pf6N2E2_4259	0.46	+0.8
4,957,846	-		Pf6N2E2_4259	0.46	+0.8
4,957,846	-		Pf6N2E2_4259	0.46	+0.3
4,957,846	-		Pf6N2E2_4259	0.46	-0.3
4,957,846	-		Pf6N2E2_4259	0.46	-1.4
4,957,846	-		Pf6N2E2_4259	0.46	-1.6
4,957,846	-		Pf6N2E2_4259	0.46	+0.6
4,957,866	+		Pf6N2E2_4259	0.48	+1.4
4,957,867	-		Pf6N2E2_4259	0.49	-0.4
4,957,923	+		Pf6N2E2_4259	0.55	+0.3
4,957,923	+		Pf6N2E2_4259	0.55	+0.6
4,957,923	+		Pf6N2E2_4259	0.55	+1.5
4,957,923	+		Pf6N2E2_4259	0.55	-0.8
4,957,923	+		Pf6N2E2_4259	0.55	+1.1
4,957,923	+		Pf6N2E2_4259	0.55	+0.7
4,957,923	+		Pf6N2E2_4259	0.55	+0.6
4,957,923	+		Pf6N2E2_4259	0.55	-0.5
4,957,924	-		Pf6N2E2_4259	0.55	+0.0
4,957,924	-		Pf6N2E2_4259	0.55	+0.2
4,957,924	-		Pf6N2E2_4259	0.55	-1.4
4,957,924	-		Pf6N2E2_4259	0.55	-1.7
4,957,924	-		Pf6N2E2_4259	0.55	-0.9
4,957,924	-		Pf6N2E2_4259	0.55	+0.3
4,957,924	-		Pf6N2E2_4259	0.55	-0.5
4,957,924	-		Pf6N2E2_4259	0.55	+1.0
4,957,924	-		Pf6N2E2_4259	0.55	+1.5
4,957,924	-		Pf6N2E2_4259	0.55	+0.9
4,957,924	-		Pf6N2E2_4259	0.55	+1.9
4,958,137	+		Pf6N2E2_4259	0.79	+0.3
4,958,137	+		Pf6N2E2_4259	0.79	-0.3
4,958,138	-		Pf6N2E2_4259	0.79	+0.1
4,958,138	-		Pf6N2E2_4259	0.79	+0.4
4,958,145	+		Pf6N2E2_4259	0.80	+0.7
4,958,145	+		Pf6N2E2_4259	0.80	+3.2
4,958,145	+		Pf6N2E2_4259	0.80	+0.9
4,958,145	+		Pf6N2E2_4259	0.80	-0.9
4,958,145	+		Pf6N2E2_4259	0.80	+0.4
4,958,145	+		Pf6N2E2_4259	0.80	-0.0
4,958,145	+		Pf6N2E2_4259	0.80	+0.8
4,958,145	+		Pf6N2E2_4259	0.80	+0.3
4,958,146	-		Pf6N2E2_4259	0.80	+0.6
4,958,146	-		Pf6N2E2_4259	0.80	+0.8
4,958,146	-		Pf6N2E2_4259	0.80	-1.7
4,958,146	-		Pf6N2E2_4259	0.80	-1.1
4,958,146	-		Pf6N2E2_4259	0.80	-0.6
4,958,146	-		Pf6N2E2_4259	0.80	+0.2
4,958,146	-		Pf6N2E2_4259	0.80	+1.2
4,958,146	-		Pf6N2E2_4259	0.80	-0.2
4,958,146	-		Pf6N2E2_4259	0.80	+0.8
4,958,146	-		Pf6N2E2_4259	0.80	+0.3
4,958,146	-		Pf6N2E2_4259	0.80	-1.8
4,958,191	+		Pf6N2E2_4259	0.86	-1.6
4,958,191	+		Pf6N2E2_4259	0.86	-1.8
4,958,191	+		Pf6N2E2_4259	0.86	-0.5
4,958,191	+		Pf6N2E2_4259	0.86	+1.5
4,958,192	-		Pf6N2E2_4259	0.86	+0.1
4,958,231	-				-0.3
4,958,231	-				-1.1
4,958,298	-				+1.1
4,958,309	+				-1.1
4,958,309	+				+0.3
4,958,309	+				-0.7
4,958,309	+				-1.0
4,958,310	-				+0.1
4,958,399	+				-0.7
4,958,399	+				-0.3
4,958,399	+				-0.2
4,958,399	+				-0.4
4,958,400	-				-0.3
4,958,447	+		Pf6N2E2_4260	0.13	+1.0
4,958,447	+		Pf6N2E2_4260	0.13	+0.3
4,958,447	+		Pf6N2E2_4260	0.13	+0.8
4,958,448	-		Pf6N2E2_4260	0.14	+1.6
4,958,448	-		Pf6N2E2_4260	0.14	-1.0
4,958,448	-		Pf6N2E2_4260	0.14	-0.5
4,958,450	+		Pf6N2E2_4260	0.14	-0.1
4,958,450	+		Pf6N2E2_4260	0.14	+0.4
4,958,450	+		Pf6N2E2_4260	0.14	-1.7
4,958,450	+		Pf6N2E2_4260	0.14	+0.3
4,958,450	+		Pf6N2E2_4260	0.14	+1.5
4,958,451	-		Pf6N2E2_4260	0.14	-1.1
4,958,451	-		Pf6N2E2_4260	0.14	+0.1
4,958,451	-		Pf6N2E2_4260	0.14	+0.4
4,958,451	-		Pf6N2E2_4260	0.14	+0.3
4,958,718	-		Pf6N2E2_4260	0.44	+0.4
4,958,879	+		Pf6N2E2_4260	0.63	-0.9
4,958,880	-		Pf6N2E2_4260	0.63	-0.1
4,958,880	-		Pf6N2E2_4260	0.63	+0.9
4,958,880	-		Pf6N2E2_4260	0.63	+0.4
4,958,908	+		Pf6N2E2_4260	0.66	-0.0
4,958,909	-		Pf6N2E2_4260	0.66	+0.1
4,958,909	-		Pf6N2E2_4260	0.66	-1.5
4,959,279	-		Pf6N2E2_4261	0.13	-1.0
4,959,279	-		Pf6N2E2_4261	0.13	+0.3
4,959,279	-		Pf6N2E2_4261	0.13	-0.3
4,959,279	-		Pf6N2E2_4261	0.13	-0.4
4,959,298	-		Pf6N2E2_4261	0.15	+0.2