Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4101

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
4,781,016	+		Pf6N2E2_4099	0.86	+1.1
4,781,016	+		Pf6N2E2_4099	0.86	+0.2
4,781,017	-		Pf6N2E2_4099	0.87	+0.5
4,781,017	-		Pf6N2E2_4099	0.87	-0.8
4,781,334	-				-1.2
4,781,334	-				+0.4
4,781,447	+		Pf6N2E2_4100	0.17	-0.8
4,781,448	-		Pf6N2E2_4100	0.17	+1.5
4,781,448	-		Pf6N2E2_4100	0.17	-0.0
4,781,498	+		Pf6N2E2_4100	0.26	+1.1
4,781,498	+		Pf6N2E2_4100	0.26	-0.5
4,781,585	+		Pf6N2E2_4100	0.42	-0.7
4,781,586	-		Pf6N2E2_4100	0.42	-2.3
4,781,717	+		Pf6N2E2_4100	0.66	+0.7
4,781,717	+		Pf6N2E2_4100	0.66	+0.4
4,781,717	+		Pf6N2E2_4100	0.66	-1.1
4,781,718	-		Pf6N2E2_4100	0.66	-0.3
4,781,718	-		Pf6N2E2_4100	0.66	-1.4
4,781,846	+		Pf6N2E2_4100	0.89	+0.3
4,781,847	-		Pf6N2E2_4100	0.89	-1.9
4,781,847	-		Pf6N2E2_4100	0.89	-1.1
4,781,889	+				-1.3
4,781,927	+				+0.7
4,781,927	+				-0.0
4,781,927	+				+0.9
4,781,927	+				+0.2
4,781,927	+				-1.3
4,781,927	+				+0.7
4,781,927	+				-0.3
4,781,927	+				-0.4
4,781,928	-				+1.6
4,781,928	-				+1.9
4,781,928	-				+0.9
4,781,928	-				-0.1
4,781,928	-				+0.7
4,781,928	-				+0.1
4,781,928	-				-2.1
4,781,928	-				-0.6
4,781,928	-				+1.3
4,781,928	-				-1.8
4,781,948	+				+0.4
4,781,948	+				+1.0
4,781,948	+				-0.5
4,781,948	+				-1.3
4,781,948	+				-1.0
4,781,948	+				-0.8
4,781,948	+				+0.8
4,781,948	+				-0.0
4,781,948	+				+0.3
4,781,949	-				-0.4
4,781,949	-				-1.7
4,781,949	-				-0.5
4,781,949	-				-0.8
4,781,949	-				-2.5
4,781,949	-				-0.2
4,781,949	-				+1.9
4,781,949	-				-0.5
4,781,949	-				+0.2
4,781,949	-				+0.7
4,781,951	+				-1.4
4,781,951	+				+0.7
4,781,951	+				+0.0
4,781,951	+				-0.3
4,781,951	+				+0.7
4,781,952	-				+1.1
4,781,952	-				-2.5
4,781,952	-				+1.1
4,781,952	-				-0.1
4,781,952	-				-0.3
4,781,952	-				-0.3
4,781,952	-				-0.6
4,782,011	+				-0.4
4,782,012	-				+1.1
4,782,104	+		Pf6N2E2_4101	0.20	+1.7
4,782,104	+		Pf6N2E2_4101	0.20	+0.2
4,782,104	+		Pf6N2E2_4101	0.20	+0.4
4,782,104	+		Pf6N2E2_4101	0.20	+0.2
4,782,104	+		Pf6N2E2_4101	0.20	+0.2
4,782,104	+		Pf6N2E2_4101	0.20	-1.0
4,782,104	+		Pf6N2E2_4101	0.20	+0.5
4,782,105	-		Pf6N2E2_4101	0.20	+0.3
4,782,105	-		Pf6N2E2_4101	0.20	+0.7
4,782,105	-		Pf6N2E2_4101	0.20	-0.5
4,782,105	-		Pf6N2E2_4101	0.20	-1.2
4,782,105	-		Pf6N2E2_4101	0.20	+0.3
4,782,105	-		Pf6N2E2_4101	0.20	+0.7
4,782,105	-		Pf6N2E2_4101	0.20	-1.5
4,782,105	-		Pf6N2E2_4101	0.20	+0.7
4,782,254	+		Pf6N2E2_4101	0.36	-0.3
4,782,254	+		Pf6N2E2_4101	0.36	+0.1
4,782,254	+		Pf6N2E2_4101	0.36	+0.9
4,782,254	+		Pf6N2E2_4101	0.36	-0.2
4,782,254	+		Pf6N2E2_4101	0.36	-1.1
4,782,254	+		Pf6N2E2_4101	0.36	+1.7
4,782,255	-		Pf6N2E2_4101	0.37	-1.7
4,782,255	-		Pf6N2E2_4101	0.37	+1.8
4,782,255	-		Pf6N2E2_4101	0.37	-0.8
4,782,255	-		Pf6N2E2_4101	0.37	+0.5
4,782,566	+		Pf6N2E2_4101	0.72	+0.9
4,782,566	+		Pf6N2E2_4101	0.72	-0.3
4,782,566	+		Pf6N2E2_4101	0.72	-1.0
4,782,567	-		Pf6N2E2_4101	0.72	+0.8
4,782,567	-		Pf6N2E2_4101	0.72	+0.8
4,782,567	-		Pf6N2E2_4101	0.72	+0.4
4,782,567	-		Pf6N2E2_4101	0.72	+1.3
4,782,567	-		Pf6N2E2_4101	0.72	+0.6
4,782,567	-		Pf6N2E2_4101	0.72	-0.6
4,782,567	-		Pf6N2E2_4101	0.72	-0.2
4,782,567	-		Pf6N2E2_4101	0.72	+0.5
4,782,567	-		Pf6N2E2_4101	0.72	-1.0
4,782,578	+		Pf6N2E2_4101	0.73	-0.7
4,782,578	+		Pf6N2E2_4101	0.73	+0.2
4,782,578	+		Pf6N2E2_4101	0.73	+1.0
4,782,578	+		Pf6N2E2_4101	0.73	-1.7
4,782,579	-		Pf6N2E2_4101	0.73	+1.1
4,782,579	-		Pf6N2E2_4101	0.73	+0.4
4,782,580	+		Pf6N2E2_4101	0.73	-0.6
4,782,580	+		Pf6N2E2_4101	0.73	+1.4
4,782,580	+		Pf6N2E2_4101	0.73	+0.1
4,782,580	+		Pf6N2E2_4101	0.73	+0.9
4,782,581	-		Pf6N2E2_4101	0.73	-0.3
4,782,581	-		Pf6N2E2_4101	0.73	+1.1
4,782,581	-		Pf6N2E2_4101	0.73	-1.8
4,782,614	+		Pf6N2E2_4101	0.77	-1.6
4,782,614	+		Pf6N2E2_4101	0.77	-1.9
4,782,806	+				-1.7
4,782,806	+				-1.4
4,782,806	+				-0.2
4,782,806	+				-0.3
4,782,807	-				+0.8
4,782,807	-				-0.6
4,782,807	-				+0.6
4,782,807	-				-0.3
4,782,807	-				+3.8
4,783,025	-				+0.3
4,783,064	+				+0.3
4,783,064	+				+0.2
4,783,064	+				+1.2
4,783,064	+				+0.7
4,783,064	+				-0.1
4,783,064	+				-0.2
4,783,064	+				+0.8
4,783,065	-				+0.9
4,783,065	-				-1.4
4,783,065	-				+0.2
4,783,066	+				+0.1
4,783,066	+				-1.9
4,783,066	+				+2.6
4,783,066	+				+0.2
4,783,066	+				+1.2
4,783,066	+				-0.7
4,783,066	+				-1.0
4,783,066	+				+0.8
4,783,066	+				-1.4
4,783,066	+				-0.6
4,783,066	+				-0.8
4,783,066	+				+0.6
4,783,066	+				-0.2
4,783,066	+				+0.0
4,783,066	+				-0.1
4,783,066	+				-1.0
4,783,066	+				-0.1
4,783,066	+				-1.1
4,783,066	+				+0.8
4,783,066	+				-1.3
4,783,066	+				+1.6
4,783,066	+				-1.0
4,783,066	+				+0.4
4,783,066	+				+0.2
4,783,066	+				+0.1
4,783,066	+				-0.7
4,783,066	+				-0.1
4,783,066	+				+0.6
4,783,066	+				+0.6
4,783,066	+				-0.9
4,783,066	+				-1.2
4,783,066	+				-1.1
4,783,066	+				+1.2
4,783,067	-				+1.1
4,783,067	-				-1.6
4,783,067	-				-2.4
4,783,067	-				+0.1
4,783,067	-				+0.5
4,783,067	-				+0.2
4,783,067	-				-0.6
4,783,067	-				-0.0
4,783,067	-				+0.1
4,783,067	-				-2.3
4,783,067	-				+0.4
4,783,067	-				+0.7
4,783,067	-				-0.0
4,783,067	-				-0.1
4,783,067	-				-0.4
4,783,067	-				-0.2
4,783,067	-				-0.5
4,783,067	-				-0.3
4,783,067	-				+0.6
4,783,067	-				-0.5
4,783,067	-				+0.2
4,783,067	-				-0.3
4,783,067	-				+0.0
4,783,067	-				+0.4
4,783,067	-				+0.1
4,783,067	-				-2.0
4,783,071	-				+0.4
4,783,223	+		Pf6N2E2_4103	0.17	-2.3
4,783,223	+		Pf6N2E2_4103	0.17	+1.3
4,783,223	+		Pf6N2E2_4103	0.17	-1.1
4,783,223	+		Pf6N2E2_4103	0.17	-0.9
4,783,224	-		Pf6N2E2_4103	0.17	+1.2
4,783,224	-		Pf6N2E2_4103	0.17	-1.6
4,783,224	-		Pf6N2E2_4103	0.17	+0.5
4,783,224	-		Pf6N2E2_4103	0.17	-1.2
4,783,302	+		Pf6N2E2_4103	0.27	-0.8
4,783,302	+		Pf6N2E2_4103	0.27	-0.4
4,783,302	+		Pf6N2E2_4103	0.27	-1.0
4,783,303	-		Pf6N2E2_4103	0.28	-1.1
4,783,303	-		Pf6N2E2_4103	0.28	-0.5
4,783,514	+		Pf6N2E2_4103	0.56	-0.2
4,783,514	+		Pf6N2E2_4103	0.56	+0.4
4,783,514	+		Pf6N2E2_4103	0.56	-0.2
4,783,514	+		Pf6N2E2_4103	0.56	+0.4
4,783,514	+		Pf6N2E2_4103	0.56	-0.5
4,783,514	+		Pf6N2E2_4103	0.56	-0.1
4,783,514	+		Pf6N2E2_4103	0.56	+0.3
4,783,515	-		Pf6N2E2_4103	0.56	-1.8
4,783,515	-		Pf6N2E2_4103	0.56	+0.2
4,783,515	-		Pf6N2E2_4103	0.56	-0.3
4,783,772	+				+0.5
4,783,772	+				-0.9