Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3679 and Pf6N2E2_3680 are separated by 81 nucleotides Pf6N2E2_3680 and Pf6N2E2_3681 are separated by 41 nucleotides
Pf6N2E2_3679 - Protein involved in catabolism of external DNA, at 4,287,702 to 4,288,541
_3679
Pf6N2E2_3680 - hypothetical protein, at 4,288,623 to 4,289,309
_3680
Pf6N2E2_3681 - VgrG protein, at 4,289,351 to 4,291,405
_3681
Position (kb)
4288
4289
4290 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4287.635 kb on + strand at 4287.635 kb on + strand at 4287.641 kb on + strand at 4287.641 kb on + strand at 4287.641 kb on + strand at 4287.642 kb on - strand at 4287.642 kb on - strand at 4287.923 kb on - strand, within Pf6N2E2_3679 at 4288.256 kb on - strand, within Pf6N2E2_3679 at 4288.258 kb on + strand, within Pf6N2E2_3679 at 4288.258 kb on + strand, within Pf6N2E2_3679 at 4288.303 kb on + strand, within Pf6N2E2_3679 at 4288.303 kb on + strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.304 kb on - strand, within Pf6N2E2_3679 at 4288.364 kb on - strand, within Pf6N2E2_3679 at 4288.402 kb on + strand, within Pf6N2E2_3679 at 4288.403 kb on - strand, within Pf6N2E2_3679 at 4288.498 kb on - strand at 4288.570 kb on - strand at 4288.645 kb on + strand at 4288.646 kb on - strand at 4288.646 kb on - strand at 4289.028 kb on + strand, within Pf6N2E2_3680 at 4289.028 kb on + strand, within Pf6N2E2_3680 at 4289.028 kb on + strand, within Pf6N2E2_3680 at 4289.029 kb on - strand, within Pf6N2E2_3680 at 4289.029 kb on - strand, within Pf6N2E2_3680 at 4289.160 kb on + strand, within Pf6N2E2_3680 at 4289.160 kb on + strand, within Pf6N2E2_3680 at 4289.160 kb on + strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.161 kb on - strand, within Pf6N2E2_3680 at 4289.164 kb on + strand, within Pf6N2E2_3680 at 4289.164 kb on + strand, within Pf6N2E2_3680 at 4289.165 kb on - strand, within Pf6N2E2_3680 at 4289.165 kb on - strand, within Pf6N2E2_3680 at 4289.165 kb on - strand, within Pf6N2E2_3680 at 4289.289 kb on + strand at 4289.290 kb on - strand at 4289.290 kb on - strand at 4289.290 kb on - strand at 4289.580 kb on - strand, within Pf6N2E2_3681 at 4289.664 kb on - strand, within Pf6N2E2_3681 at 4289.664 kb on - strand, within Pf6N2E2_3681 at 4289.664 kb on - strand, within Pf6N2E2_3681 at 4289.728 kb on + strand, within Pf6N2E2_3681 at 4289.728 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.922 kb on + strand, within Pf6N2E2_3681 at 4289.923 kb on - strand, within Pf6N2E2_3681 at 4289.923 kb on - strand, within Pf6N2E2_3681 at 4289.968 kb on - strand, within Pf6N2E2_3681 at 4289.968 kb on - strand, within Pf6N2E2_3681
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 4,287,635 + +0.5 4,287,635 + +2.3 4,287,641 + +1.8 4,287,641 + +0.0 4,287,641 + -1.1 4,287,642 - -0.0 4,287,642 - +0.5 4,287,923 - Pf6N2E2_3679 0.26 +0.4 4,288,256 - Pf6N2E2_3679 0.66 -0.9 4,288,258 + Pf6N2E2_3679 0.66 -0.3 4,288,258 + Pf6N2E2_3679 0.66 +1.1 4,288,303 + Pf6N2E2_3679 0.72 -0.4 4,288,303 + Pf6N2E2_3679 0.72 +2.5 4,288,304 - Pf6N2E2_3679 0.72 +0.3 4,288,304 - Pf6N2E2_3679 0.72 -0.9 4,288,304 - Pf6N2E2_3679 0.72 -1.1 4,288,364 - Pf6N2E2_3679 0.79 -0.6 4,288,402 + Pf6N2E2_3679 0.83 +1.1 4,288,403 - Pf6N2E2_3679 0.83 -2.1 4,288,498 - -0.3 4,288,570 - -2.0 4,288,645 + -1.9 4,288,646 - +2.9 4,288,646 - +0.6 4,289,028 + Pf6N2E2_3680 0.59 +2.0 4,289,028 + Pf6N2E2_3680 0.59 -0.8 4,289,028 + Pf6N2E2_3680 0.59 +0.3 4,289,029 - Pf6N2E2_3680 0.59 -0.8 4,289,029 - Pf6N2E2_3680 0.59 +0.9 4,289,160 + Pf6N2E2_3680 0.78 -0.6 4,289,160 + Pf6N2E2_3680 0.78 -0.8 4,289,160 + Pf6N2E2_3680 0.78 -0.6 4,289,161 - Pf6N2E2_3680 0.78 -0.5 4,289,161 - Pf6N2E2_3680 0.78 -0.7 4,289,161 - Pf6N2E2_3680 0.78 +0.9 4,289,161 - Pf6N2E2_3680 0.78 -0.3 4,289,161 - Pf6N2E2_3680 0.78 -0.9 4,289,161 - Pf6N2E2_3680 0.78 -0.4 4,289,164 + Pf6N2E2_3680 0.79 -0.7 4,289,164 + Pf6N2E2_3680 0.79 -0.8 4,289,165 - Pf6N2E2_3680 0.79 +2.3 4,289,165 - Pf6N2E2_3680 0.79 -0.2 4,289,165 - Pf6N2E2_3680 0.79 +1.0 4,289,289 + +0.2 4,289,290 - -1.6 4,289,290 - -0.2 4,289,290 - +0.8 4,289,580 - Pf6N2E2_3681 0.11 -0.1 4,289,664 - Pf6N2E2_3681 0.15 -0.6 4,289,664 - Pf6N2E2_3681 0.15 -0.5 4,289,664 - Pf6N2E2_3681 0.15 -0.2 4,289,728 + Pf6N2E2_3681 0.18 -0.8 4,289,728 + Pf6N2E2_3681 0.18 +0.3 4,289,922 + Pf6N2E2_3681 0.28 +0.7 4,289,922 + Pf6N2E2_3681 0.28 +1.3 4,289,922 + Pf6N2E2_3681 0.28 +0.4 4,289,922 + Pf6N2E2_3681 0.28 -0.2 4,289,922 + Pf6N2E2_3681 0.28 -1.0 4,289,922 + Pf6N2E2_3681 0.28 -0.1 4,289,922 + Pf6N2E2_3681 0.28 +0.7 4,289,922 + Pf6N2E2_3681 0.28 +0.5 4,289,923 - Pf6N2E2_3681 0.28 -0.8 4,289,923 - Pf6N2E2_3681 0.28 -1.3 4,289,968 - Pf6N2E2_3681 0.30 +0.1 4,289,968 - Pf6N2E2_3681 0.30 -0.2
Or see this region's nucleotide sequence