Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3367 and Pf6N2E2_3368 are separated by 322 nucleotides Pf6N2E2_3368 and Pf6N2E2_3369 are separated by 101 nucleotides Pf6N2E2_3369 and Pf6N2E2_3370 are separated by 241 nucleotides
Pf6N2E2_3367 - Chaperone protein DnaJ, at 3,899,271 to 3,900,395
_3367
Pf6N2E2_3368 - Chaperone protein DnaK, at 3,900,718 to 3,902,634
_3368
Pf6N2E2_3369 - Heat shock protein GrpE, at 3,902,736 to 3,903,302
_3369
Pf6N2E2_3370 - DNA repair protein RecN, at 3,903,544 to 3,905,217
_3370
Position (kb)
3900
3901
3902
3903 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3899.765 kb on - strand, within Pf6N2E2_3367 at 3899.765 kb on - strand, within Pf6N2E2_3367 at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.412 kb on - strand at 3900.414 kb on - strand at 3900.414 kb on - strand at 3900.510 kb on - strand at 3900.720 kb on - strand at 3900.720 kb on - strand at 3900.828 kb on - strand at 3902.711 kb on - strand at 3902.711 kb on - strand at 3902.711 kb on - strand at 3902.711 kb on - strand at 3902.711 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.713 kb on - strand at 3902.726 kb on - strand at 3902.726 kb on - strand at 3902.754 kb on - strand at 3902.754 kb on - strand at 3902.754 kb on - strand at 3902.754 kb on - strand at 3903.358 kb on - strand at 3903.400 kb on - strand at 3903.400 kb on - strand at 3903.400 kb on - strand at 3903.423 kb on - strand at 3903.454 kb on + strand at 3903.455 kb on - strand at 3903.455 kb on - strand at 3903.472 kb on + strand at 3903.543 kb on + strand at 3903.543 kb on + strand at 3903.543 kb on + strand at 3903.543 kb on + strand at 3903.544 kb on - strand at 3903.563 kb on + strand at 3903.564 kb on - strand at 3903.571 kb on + strand at 3903.571 kb on + strand at 3903.571 kb on + strand at 3903.571 kb on + strand at 3903.571 kb on + strand at 3903.572 kb on - strand at 3903.572 kb on - strand at 3903.572 kb on - strand at 3903.572 kb on - strand at 3903.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,899,765 - Pf6N2E2_3367 0.44 +0.9 3,899,765 - Pf6N2E2_3367 0.44 +1.9 3,900,412 - -1.1 3,900,412 - -1.1 3,900,412 - -2.4 3,900,412 - -2.6 3,900,412 - -0.2 3,900,412 - -0.3 3,900,412 - -1.8 3,900,412 - -2.6 3,900,414 - +0.7 3,900,414 - +0.2 3,900,510 - +2.9 3,900,720 - +0.7 3,900,720 - -1.1 3,900,828 - -1.2 3,902,711 - +0.1 3,902,711 - +3.2 3,902,711 - -1.1 3,902,711 - -2.0 3,902,711 - +3.7 3,902,713 - -1.6 3,902,713 - -2.7 3,902,713 - -0.1 3,902,713 - +0.5 3,902,713 - -0.4 3,902,713 - -0.4 3,902,713 - -1.2 3,902,713 - -0.8 3,902,713 - +0.7 3,902,713 - -0.3 3,902,726 - +0.8 3,902,726 - +0.4 3,902,754 - -0.1 3,902,754 - -2.6 3,902,754 - -2.6 3,902,754 - -1.9 3,903,358 - -0.6 3,903,400 - -0.5 3,903,400 - +0.4 3,903,400 - +1.3 3,903,423 - -0.1 3,903,454 + -1.9 3,903,455 - +0.5 3,903,455 - -0.9 3,903,472 + -0.9 3,903,543 + -1.2 3,903,543 + -0.7 3,903,543 + -0.1 3,903,543 + -1.0 3,903,544 - -1.1 3,903,563 + +1.5 3,903,564 - +0.3 3,903,571 + -0.4 3,903,571 + +1.2 3,903,571 + -0.5 3,903,571 + -2.5 3,903,571 + +0.2 3,903,572 - -1.9 3,903,572 - -0.2 3,903,572 - -1.9 3,903,572 - -0.1 3,903,572 - +2.0
Or see this region's nucleotide sequence