Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3367

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3366 and Pf6N2E2_3367 are separated by 9 nucleotidesPf6N2E2_3367 and Pf6N2E2_3368 are separated by 322 nucleotides Pf6N2E2_3366 - 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8), at 3,898,455 to 3,899,261 _3366 Pf6N2E2_3367 - Chaperone protein DnaJ, at 3,899,271 to 3,900,395 _3367 Pf6N2E2_3368 - Chaperone protein DnaK, at 3,900,718 to 3,902,634 _3368 Position (kb) 3899 3900 3901Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3898.295 kb on - strandat 3898.412 kb on + strandat 3898.413 kb on - strandat 3898.427 kb on + strandat 3898.427 kb on + strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.428 kb on - strandat 3898.430 kb on - strandat 3899.765 kb on - strand, within Pf6N2E2_3367at 3899.765 kb on - strand, within Pf6N2E2_3367at 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.412 kb on - strandat 3900.414 kb on - strandat 3900.414 kb on - strandat 3900.510 kb on - strandat 3900.720 kb on - strandat 3900.720 kb on - strandat 3900.828 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,898,295 - +1.4
3,898,412 + -0.7
3,898,413 - +0.7
3,898,427 + +0.7
3,898,427 + -0.4
3,898,428 - -0.7
3,898,428 - -1.5
3,898,428 - +0.6
3,898,428 - -0.6
3,898,430 - -1.8
3,899,765 - Pf6N2E2_3367 0.44 +0.9
3,899,765 - Pf6N2E2_3367 0.44 +1.9
3,900,412 - -1.1
3,900,412 - -1.1
3,900,412 - -2.4
3,900,412 - -2.6
3,900,412 - -0.2
3,900,412 - -0.3
3,900,412 - -1.8
3,900,412 - -2.6
3,900,414 - +0.7
3,900,414 - +0.2
3,900,510 - +2.9
3,900,720 - +0.7
3,900,720 - -1.1
3,900,828 - -1.2

Or see this region's nucleotide sequence