Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_306 and Pf6N2E2_307 overlap by 1 nucleotides Pf6N2E2_307 and Pf6N2E2_308 overlap by 1 nucleotides Pf6N2E2_308 and Pf6N2E2_309 are separated by 219 nucleotides Pf6N2E2_309 and Pf6N2E2_310 are separated by 2 nucleotides Pf6N2E2_310 and Pf6N2E2_311 are separated by 141 nucleotides Pf6N2E2_311 and Pf6N2E2_312 are separated by 80 nucleotides
Pf6N2E2_306 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 376,334 to 376,627
_306
Pf6N2E2_307 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 376,627 to 376,989
_307
Pf6N2E2_308 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 376,989 to 377,381
_308
Pf6N2E2_309 - FIG00957702: hypothetical protein, at 377,601 to 377,930
_309
Pf6N2E2_310 - hypothetical protein, at 377,933 to 378,193
_310
Pf6N2E2_311 - hypothetical protein, at 378,335 to 378,685
_311
Pf6N2E2_312 - Histone acetyltransferase HPA2 and related acetyltransferases, at 378,766 to 379,197
_312
Position (kb) 377
378 Strain fitness (log2 ratio) -2
-1
0
1 at 377.602 kb on + strand at 377.603 kb on - strand at 377.629 kb on + strand at 377.696 kb on - strand, within Pf6N2E2_309 at 377.696 kb on - strand, within Pf6N2E2_309 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.012 kb on + strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.013 kb on - strand, within Pf6N2E2_310 at 378.069 kb on + strand, within Pf6N2E2_310 at 378.070 kb on - strand, within Pf6N2E2_310 at 378.154 kb on - strand, within Pf6N2E2_310 at 378.171 kb on + strand at 378.172 kb on - strand at 378.172 kb on - strand at 378.302 kb on + strand at 378.422 kb on + strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.423 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.558 kb on - strand, within Pf6N2E2_311 at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.691 kb on + strand at 378.692 kb on - strand at 378.733 kb on + strand at 378.733 kb on + strand at 378.768 kb on - strand at 378.863 kb on + strand, within Pf6N2E2_312
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 377,602 + +1.6 377,603 - -1.0 377,629 + -2.0 377,696 - Pf6N2E2_309 0.29 -0.6 377,696 - Pf6N2E2_309 0.29 -0.4 378,012 + Pf6N2E2_310 0.30 -1.3 378,012 + Pf6N2E2_310 0.30 -1.9 378,012 + Pf6N2E2_310 0.30 -1.1 378,012 + Pf6N2E2_310 0.30 +0.1 378,012 + Pf6N2E2_310 0.30 -1.0 378,013 - Pf6N2E2_310 0.31 -0.7 378,013 - Pf6N2E2_310 0.31 +0.7 378,013 - Pf6N2E2_310 0.31 -1.3 378,069 + Pf6N2E2_310 0.52 +0.1 378,070 - Pf6N2E2_310 0.52 -0.1 378,154 - Pf6N2E2_310 0.85 -1.2 378,171 + -2.0 378,172 - -2.0 378,172 - +0.6 378,302 + -0.1 378,422 + Pf6N2E2_311 0.25 -0.1 378,423 - Pf6N2E2_311 0.25 +0.7 378,423 - Pf6N2E2_311 0.25 -1.5 378,423 - Pf6N2E2_311 0.25 +0.4 378,423 - Pf6N2E2_311 0.25 +1.3 378,558 - Pf6N2E2_311 0.64 -0.2 378,558 - Pf6N2E2_311 0.64 -1.4 378,691 + +1.7 378,691 + +0.4 378,691 + -1.4 378,691 + +0.3 378,692 - +1.1 378,733 + +0.9 378,733 + +1.0 378,768 - +0.2 378,863 + Pf6N2E2_312 0.22 -1.2
Or see this region's nucleotide sequence