Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2582

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotidesPf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotidesPf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690 _2580 Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127 _2581 Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586 _2582 Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627 _2583 Position (kb) 3040 3041Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3039.207 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.335 kb on + strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.336 kb on - strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.569 kb on + strand, within Pf6N2E2_2580at 3039.692 kb on - strandat 3039.692 kb on - strandat 3039.855 kb on + strand, within Pf6N2E2_2581at 3040.242 kb on + strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.243 kb on - strand, within Pf6N2E2_2582at 3040.617 kb on - strandat 3040.617 kb on - strandat 3040.641 kb on + strandat 3040.641 kb on + strandat 3040.641 kb on + strandat 3040.972 kb on + strand, within Pf6N2E2_2583at 3040.973 kb on - strand, within Pf6N2E2_2583at 3041.095 kb on + strand, within Pf6N2E2_2583at 3041.096 kb on - strand, within Pf6N2E2_2583at 3041.338 kb on + strand, within Pf6N2E2_2583at 3041.339 kb on - strand, within Pf6N2E2_2583at 3041.353 kb on + strand, within Pf6N2E2_2583at 3041.353 kb on + strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.354 kb on - strand, within Pf6N2E2_2583at 3041.515 kb on + strand, within Pf6N2E2_2583

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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3,039,207 + Pf6N2E2_2580 0.35 -2.4
3,039,335 + Pf6N2E2_2580 0.53 -1.5
3,039,335 + Pf6N2E2_2580 0.53 -1.7
3,039,336 - Pf6N2E2_2580 0.53 -0.9
3,039,336 - Pf6N2E2_2580 0.53 -1.1
3,039,569 + Pf6N2E2_2580 0.84 -2.5
3,039,569 + Pf6N2E2_2580 0.84 -1.6
3,039,692 - -2.3
3,039,692 - -2.1
3,039,855 + Pf6N2E2_2581 0.38 -0.9
3,040,242 + Pf6N2E2_2582 0.25 -2.2
3,040,243 - Pf6N2E2_2582 0.25 -1.0
3,040,243 - Pf6N2E2_2582 0.25 -2.6
3,040,243 - Pf6N2E2_2582 0.25 -1.8
3,040,617 - +0.1
3,040,617 - -1.0
3,040,641 + -1.7
3,040,641 + -3.0
3,040,641 + -2.2
3,040,972 + Pf6N2E2_2583 0.32 -0.7
3,040,973 - Pf6N2E2_2583 0.32 -3.5
3,041,095 + Pf6N2E2_2583 0.45 -1.9
3,041,096 - Pf6N2E2_2583 0.45 -2.1
3,041,338 + Pf6N2E2_2583 0.70 -3.2
3,041,339 - Pf6N2E2_2583 0.70 -2.9
3,041,353 + Pf6N2E2_2583 0.72 -2.3
3,041,353 + Pf6N2E2_2583 0.72 -2.6
3,041,354 - Pf6N2E2_2583 0.72 -2.3
3,041,354 - Pf6N2E2_2583 0.72 -3.1
3,041,354 - Pf6N2E2_2583 0.72 -2.4
3,041,354 - Pf6N2E2_2583 0.72 -4.0
3,041,354 - Pf6N2E2_2583 0.72 -2.1
3,041,515 + Pf6N2E2_2583 0.88 -0.7

Or see this region's nucleotide sequence