Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides Pf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotides Pf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127
_2581
Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586
_2582
Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627
_2583
Position (kb)
3040
3041 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3039.207 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.335 kb on + strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.336 kb on - strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.569 kb on + strand, within Pf6N2E2_2580 at 3039.692 kb on - strand at 3039.692 kb on - strand at 3039.855 kb on + strand, within Pf6N2E2_2581 at 3040.242 kb on + strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.617 kb on - strand at 3040.617 kb on - strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.972 kb on + strand, within Pf6N2E2_2583 at 3040.973 kb on - strand, within Pf6N2E2_2583 at 3041.095 kb on + strand, within Pf6N2E2_2583 at 3041.096 kb on - strand, within Pf6N2E2_2583 at 3041.338 kb on + strand, within Pf6N2E2_2583 at 3041.339 kb on - strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.515 kb on + strand, within Pf6N2E2_2583
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 3,039,207 + Pf6N2E2_2580 0.35 -2.4 3,039,335 + Pf6N2E2_2580 0.53 -1.5 3,039,335 + Pf6N2E2_2580 0.53 -1.7 3,039,336 - Pf6N2E2_2580 0.53 -0.9 3,039,336 - Pf6N2E2_2580 0.53 -1.1 3,039,569 + Pf6N2E2_2580 0.84 -2.5 3,039,569 + Pf6N2E2_2580 0.84 -1.6 3,039,692 - -2.3 3,039,692 - -2.1 3,039,855 + Pf6N2E2_2581 0.38 -0.9 3,040,242 + Pf6N2E2_2582 0.25 -2.2 3,040,243 - Pf6N2E2_2582 0.25 -1.0 3,040,243 - Pf6N2E2_2582 0.25 -2.6 3,040,243 - Pf6N2E2_2582 0.25 -1.8 3,040,617 - +0.1 3,040,617 - -1.0 3,040,641 + -1.7 3,040,641 + -3.0 3,040,641 + -2.2 3,040,972 + Pf6N2E2_2583 0.32 -0.7 3,040,973 - Pf6N2E2_2583 0.32 -3.5 3,041,095 + Pf6N2E2_2583 0.45 -1.9 3,041,096 - Pf6N2E2_2583 0.45 -2.1 3,041,338 + Pf6N2E2_2583 0.70 -3.2 3,041,339 - Pf6N2E2_2583 0.70 -2.9 3,041,353 + Pf6N2E2_2583 0.72 -2.3 3,041,353 + Pf6N2E2_2583 0.72 -2.6 3,041,354 - Pf6N2E2_2583 0.72 -2.3 3,041,354 - Pf6N2E2_2583 0.72 -3.1 3,041,354 - Pf6N2E2_2583 0.72 -2.4 3,041,354 - Pf6N2E2_2583 0.72 -4.0 3,041,354 - Pf6N2E2_2583 0.72 -2.1 3,041,515 + Pf6N2E2_2583 0.88 -0.7
Or see this region's nucleotide sequence