Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_239 and Pf6N2E2_240 are separated by 171 nucleotides Pf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotides Pf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotides
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519
_241
Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202
_242
Position (kb) 301
302 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand at 300.295 kb on + strand, within Pf6N2E2_239 at 300.295 kb on + strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.559 kb on + strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.709 kb on - strand, within Pf6N2E2_239 at 300.749 kb on - strand, within Pf6N2E2_239 at 300.764 kb on - strand at 300.764 kb on - strand at 300.773 kb on + strand at 300.830 kb on - strand at 300.835 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.845 kb on - strand at 300.845 kb on - strand at 300.894 kb on + strand at 300.894 kb on + strand at 300.960 kb on - strand at 300.960 kb on - strand at 301.124 kb on + strand, within Pf6N2E2_240 at 301.124 kb on + strand, within Pf6N2E2_240 at 301.125 kb on - strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.191 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.258 kb on + strand, within Pf6N2E2_240 at 301.334 kb on + strand, within Pf6N2E2_240 at 301.334 kb on + strand, within Pf6N2E2_240 at 301.335 kb on - strand, within Pf6N2E2_240 at 301.423 kb on - strand, within Pf6N2E2_240 at 301.641 kb on + strand, within Pf6N2E2_240 at 301.642 kb on - strand, within Pf6N2E2_240 at 301.952 kb on + strand at 301.981 kb on + strand at 302.115 kb on - strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.244 kb on + strand at 302.336 kb on - strand at 302.481 kb on + strand, within Pf6N2E2_241 at 302.540 kb on + strand at 302.540 kb on + strand at 302.612 kb on + strand, within Pf6N2E2_242 at 302.677 kb on + strand, within Pf6N2E2_242 at 302.682 kb on - strand, within Pf6N2E2_242 at 302.840 kb on - strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 300,010 - Pf6N2E2_238 0.28 -0.8 300,172 + +1.4 300,183 + +0.0 300,184 - +0.1 300,184 - +1.0 300,295 + Pf6N2E2_239 0.24 +0.4 300,295 + Pf6N2E2_239 0.24 +2.4 300,296 - Pf6N2E2_239 0.24 +1.8 300,296 - Pf6N2E2_239 0.24 -0.4 300,297 + Pf6N2E2_239 0.24 +0.9 300,297 + Pf6N2E2_239 0.24 +0.1 300,297 + Pf6N2E2_239 0.24 -1.3 300,298 - Pf6N2E2_239 0.24 -0.3 300,298 - Pf6N2E2_239 0.24 +0.9 300,298 - Pf6N2E2_239 0.24 -1.0 300,298 - Pf6N2E2_239 0.24 +0.4 300,298 - Pf6N2E2_239 0.24 -0.1 300,298 - Pf6N2E2_239 0.24 +0.4 300,298 - Pf6N2E2_239 0.24 -0.1 300,482 - Pf6N2E2_239 0.50 -0.2 300,482 - Pf6N2E2_239 0.50 -0.4 300,506 - Pf6N2E2_239 0.54 +0.6 300,506 - Pf6N2E2_239 0.54 -1.1 300,559 + Pf6N2E2_239 0.61 -1.8 300,595 - Pf6N2E2_239 0.66 +0.0 300,595 - Pf6N2E2_239 0.66 +1.1 300,707 - Pf6N2E2_239 0.82 -0.7 300,707 - Pf6N2E2_239 0.82 -0.1 300,709 - Pf6N2E2_239 0.82 -0.9 300,749 - Pf6N2E2_239 0.88 +2.3 300,764 - -0.1 300,764 - -0.5 300,773 + +0.6 300,830 - +0.2 300,835 + +0.6 300,844 + -0.7 300,844 + +0.0 300,844 + +1.1 300,844 + +1.6 300,845 - +1.3 300,845 - -1.7 300,894 + +0.4 300,894 + +0.1 300,960 - -0.7 300,960 - -1.1 301,124 + Pf6N2E2_240 0.14 +1.1 301,124 + Pf6N2E2_240 0.14 +2.1 301,125 - Pf6N2E2_240 0.14 +2.1 301,191 + Pf6N2E2_240 0.21 -0.3 301,191 + Pf6N2E2_240 0.21 +0.3 301,191 + Pf6N2E2_240 0.21 -0.3 301,258 + Pf6N2E2_240 0.29 +0.3 301,258 + Pf6N2E2_240 0.29 -0.8 301,258 + Pf6N2E2_240 0.29 +1.2 301,334 + Pf6N2E2_240 0.37 -0.3 301,334 + Pf6N2E2_240 0.37 +0.5 301,335 - Pf6N2E2_240 0.37 +3.4 301,423 - Pf6N2E2_240 0.47 -0.9 301,641 + Pf6N2E2_240 0.72 +0.0 301,642 - Pf6N2E2_240 0.72 -1.2 301,952 + +1.2 301,981 + +1.1 302,115 - +1.7 302,157 + -1.8 302,157 + -0.4 302,157 + -0.2 302,244 + -1.3 302,336 - +0.9 302,481 + Pf6N2E2_241 0.73 -0.4 302,540 + -0.6 302,540 + -0.2 302,612 + Pf6N2E2_242 0.14 +3.1 302,677 + Pf6N2E2_242 0.23 +0.5 302,682 - Pf6N2E2_242 0.24 +0.7 302,840 - Pf6N2E2_242 0.47 -1.2 302,843 + Pf6N2E2_242 0.47 -0.8 302,843 + Pf6N2E2_242 0.47 -0.1 302,843 + Pf6N2E2_242 0.47 -0.7 302,843 + Pf6N2E2_242 0.47 -0.4 302,843 + Pf6N2E2_242 0.47 -0.1 302,843 + Pf6N2E2_242 0.47 -0.8 302,843 + Pf6N2E2_242 0.47 -0.5 302,843 + Pf6N2E2_242 0.47 -0.1 302,844 - Pf6N2E2_242 0.48 -1.4 302,844 - Pf6N2E2_242 0.48 +1.3
Or see this region's nucleotide sequence