Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2378 and Pf6N2E2_2379 overlap by 4 nucleotides Pf6N2E2_2379 and Pf6N2E2_2380 overlap by 1 nucleotides Pf6N2E2_2380 and Pf6N2E2_2381 are separated by 10 nucleotides Pf6N2E2_2381 and Pf6N2E2_2382 are separated by 197 nucleotides
Pf6N2E2_2378 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,814,933 to 2,817,386
_2378
Pf6N2E2_2379 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 2,817,383 to 2,817,700
_2379
Pf6N2E2_2380 - Nitrate reductase (EC 1.7.99.4) (from data) , at 2,817,700 to 2,820,417
_2380
Pf6N2E2_2381 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 2,820,428 to 2,821,183
_2381
Pf6N2E2_2382 - Major porin and structural outer membrane porin OprF, at 2,821,381 to 2,822,415
_2382
Position (kb)
2817
2818
2819
2820
2821 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.713 kb on - strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.719 kb on + strand, within Pf6N2E2_2378 at 2816.720 kb on - strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.048 kb on + strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.049 kb on - strand, within Pf6N2E2_2378 at 2817.130 kb on - strand, within Pf6N2E2_2378 at 2817.171 kb on + strand at 2817.171 kb on + strand at 2817.172 kb on - strand at 2817.172 kb on - strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.192 kb on + strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.193 kb on - strand at 2817.398 kb on - strand at 2817.672 kb on + strand at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.012 kb on + strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.013 kb on - strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.042 kb on + strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.043 kb on - strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.045 kb on + strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.046 kb on - strand, within Pf6N2E2_2380 at 2818.086 kb on + strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380 at 2818.136 kb on - strand, within Pf6N2E2_2380 at 2818.483 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.643 kb on + strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2818.644 kb on - strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.239 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.362 kb on + strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.363 kb on - strand, within Pf6N2E2_2380 at 2819.730 kb on + strand, within Pf6N2E2_2380 at 2819.896 kb on + strand, within Pf6N2E2_2380 at 2819.896 kb on + strand, within Pf6N2E2_2380 at 2820.031 kb on + strand, within Pf6N2E2_2380 at 2820.072 kb on - strand, within Pf6N2E2_2380 at 2820.288 kb on + strand at 2820.288 kb on + strand at 2820.427 kb on + strand at 2820.428 kb on - strand at 2820.478 kb on - strand at 2820.478 kb on - strand at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.926 kb on + strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2820.927 kb on - strand, within Pf6N2E2_2381 at 2821.179 kb on + strand at 2821.179 kb on + strand at 2821.179 kb on + strand at 2821.180 kb on - strand at 2821.291 kb on + strand at 2821.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,816,713 - Pf6N2E2_2378 0.73 -0.0 2,816,713 - Pf6N2E2_2378 0.73 +0.2 2,816,713 - Pf6N2E2_2378 0.73 +0.7 2,816,713 - Pf6N2E2_2378 0.73 +0.3 2,816,719 + Pf6N2E2_2378 0.73 +0.7 2,816,719 + Pf6N2E2_2378 0.73 -1.0 2,816,720 - Pf6N2E2_2378 0.73 -2.9 2,817,048 + Pf6N2E2_2378 0.86 -0.3 2,817,048 + Pf6N2E2_2378 0.86 -0.4 2,817,048 + Pf6N2E2_2378 0.86 +0.9 2,817,048 + Pf6N2E2_2378 0.86 +0.2 2,817,048 + Pf6N2E2_2378 0.86 -1.6 2,817,048 + Pf6N2E2_2378 0.86 -0.7 2,817,048 + Pf6N2E2_2378 0.86 +1.3 2,817,048 + Pf6N2E2_2378 0.86 +0.7 2,817,049 - Pf6N2E2_2378 0.86 -0.8 2,817,049 - Pf6N2E2_2378 0.86 +0.3 2,817,049 - Pf6N2E2_2378 0.86 +0.1 2,817,049 - Pf6N2E2_2378 0.86 +1.4 2,817,049 - Pf6N2E2_2378 0.86 -0.3 2,817,049 - Pf6N2E2_2378 0.86 -0.7 2,817,049 - Pf6N2E2_2378 0.86 -0.1 2,817,130 - Pf6N2E2_2378 0.90 -1.4 2,817,171 + +1.1 2,817,171 + -0.0 2,817,172 - -2.0 2,817,172 - -1.8 2,817,192 + +0.9 2,817,192 + +1.5 2,817,192 + +0.0 2,817,192 + -0.7 2,817,192 + -1.3 2,817,192 + -0.9 2,817,192 + -0.3 2,817,193 - -1.5 2,817,193 - -1.3 2,817,193 - +0.2 2,817,193 - +0.9 2,817,398 - +0.5 2,817,672 + -1.2 2,818,012 + Pf6N2E2_2380 0.11 +0.5 2,818,012 + Pf6N2E2_2380 0.11 -0.0 2,818,012 + Pf6N2E2_2380 0.11 -1.3 2,818,013 - Pf6N2E2_2380 0.12 -0.2 2,818,013 - Pf6N2E2_2380 0.12 -0.3 2,818,013 - Pf6N2E2_2380 0.12 -0.3 2,818,042 + Pf6N2E2_2380 0.13 -0.7 2,818,042 + Pf6N2E2_2380 0.13 +0.8 2,818,042 + Pf6N2E2_2380 0.13 +1.3 2,818,043 - Pf6N2E2_2380 0.13 +0.9 2,818,043 - Pf6N2E2_2380 0.13 -1.0 2,818,043 - Pf6N2E2_2380 0.13 +0.3 2,818,043 - Pf6N2E2_2380 0.13 -0.8 2,818,043 - Pf6N2E2_2380 0.13 -0.5 2,818,043 - Pf6N2E2_2380 0.13 +0.1 2,818,043 - Pf6N2E2_2380 0.13 +2.0 2,818,043 - Pf6N2E2_2380 0.13 -0.7 2,818,043 - Pf6N2E2_2380 0.13 +0.9 2,818,043 - Pf6N2E2_2380 0.13 +0.1 2,818,043 - Pf6N2E2_2380 0.13 -1.0 2,818,045 + Pf6N2E2_2380 0.13 +2.4 2,818,045 + Pf6N2E2_2380 0.13 -0.9 2,818,045 + Pf6N2E2_2380 0.13 -0.0 2,818,046 - Pf6N2E2_2380 0.13 -1.1 2,818,046 - Pf6N2E2_2380 0.13 +1.8 2,818,046 - Pf6N2E2_2380 0.13 +1.0 2,818,046 - Pf6N2E2_2380 0.13 -0.1 2,818,046 - Pf6N2E2_2380 0.13 -0.5 2,818,046 - Pf6N2E2_2380 0.13 -0.8 2,818,046 - Pf6N2E2_2380 0.13 -2.0 2,818,086 + Pf6N2E2_2380 0.14 +1.6 2,818,136 - Pf6N2E2_2380 0.16 +0.7 2,818,136 - Pf6N2E2_2380 0.16 -0.4 2,818,483 + Pf6N2E2_2380 0.29 +1.5 2,818,643 + Pf6N2E2_2380 0.35 +0.4 2,818,643 + Pf6N2E2_2380 0.35 -0.2 2,818,643 + Pf6N2E2_2380 0.35 -1.9 2,818,643 + Pf6N2E2_2380 0.35 -0.2 2,818,643 + Pf6N2E2_2380 0.35 +1.0 2,818,643 + Pf6N2E2_2380 0.35 +0.2 2,818,643 + Pf6N2E2_2380 0.35 -0.1 2,818,643 + Pf6N2E2_2380 0.35 +0.8 2,818,644 - Pf6N2E2_2380 0.35 +1.0 2,818,644 - Pf6N2E2_2380 0.35 -1.1 2,818,644 - Pf6N2E2_2380 0.35 +1.5 2,818,644 - Pf6N2E2_2380 0.35 +0.1 2,818,644 - Pf6N2E2_2380 0.35 +0.9 2,819,239 + Pf6N2E2_2380 0.57 +1.2 2,819,239 + Pf6N2E2_2380 0.57 +0.8 2,819,239 + Pf6N2E2_2380 0.57 +1.1 2,819,239 + Pf6N2E2_2380 0.57 +0.5 2,819,362 + Pf6N2E2_2380 0.61 +2.4 2,819,362 + Pf6N2E2_2380 0.61 +1.7 2,819,362 + Pf6N2E2_2380 0.61 -0.9 2,819,363 - Pf6N2E2_2380 0.61 -1.3 2,819,363 - Pf6N2E2_2380 0.61 +0.5 2,819,363 - Pf6N2E2_2380 0.61 +1.5 2,819,730 + Pf6N2E2_2380 0.75 +1.2 2,819,896 + Pf6N2E2_2380 0.81 -0.5 2,819,896 + Pf6N2E2_2380 0.81 +0.6 2,820,031 + Pf6N2E2_2380 0.86 +0.3 2,820,072 - Pf6N2E2_2380 0.87 -0.2 2,820,288 + -2.0 2,820,288 + +3.2 2,820,427 + +1.4 2,820,428 - +2.3 2,820,478 - -0.4 2,820,478 - +0.1 2,820,926 + Pf6N2E2_2381 0.66 +0.4 2,820,926 + Pf6N2E2_2381 0.66 +0.4 2,820,926 + Pf6N2E2_2381 0.66 +1.4 2,820,926 + Pf6N2E2_2381 0.66 -0.5 2,820,926 + Pf6N2E2_2381 0.66 -2.7 2,820,926 + Pf6N2E2_2381 0.66 -0.1 2,820,926 + Pf6N2E2_2381 0.66 +0.3 2,820,927 - Pf6N2E2_2381 0.66 +0.7 2,820,927 - Pf6N2E2_2381 0.66 -0.3 2,820,927 - Pf6N2E2_2381 0.66 +0.1 2,820,927 - Pf6N2E2_2381 0.66 -0.3 2,820,927 - Pf6N2E2_2381 0.66 +2.8 2,820,927 - Pf6N2E2_2381 0.66 -1.5 2,820,927 - Pf6N2E2_2381 0.66 +0.7 2,821,179 + +0.1 2,821,179 + -0.0 2,821,179 + +0.6 2,821,180 - +0.6 2,821,291 + -0.7 2,821,291 + +1.5
Or see this region's nucleotide sequence