Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2305 and Pf6N2E2_2306 are separated by 10 nucleotides Pf6N2E2_2306 and Pf6N2E2_2307 are separated by 27 nucleotides Pf6N2E2_2307 and Pf6N2E2_2308 overlap by 4 nucleotides
Pf6N2E2_2305 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 2,730,529 to 2,731,953
_2305
Pf6N2E2_2306 - Cytochrome c oxidase subunit CcoO (EC 1.9.3.1), at 2,731,964 to 2,732,572
_2306
Pf6N2E2_2307 - Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1), at 2,732,600 to 2,732,779
_2307
Pf6N2E2_2308 - Cytochrome c oxidase subunit CcoP (EC 1.9.3.1), at 2,732,776 to 2,733,732
_2308
Position (kb)
2732
2733 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2731.615 kb on + strand, within Pf6N2E2_2305 at 2731.616 kb on - strand, within Pf6N2E2_2305 at 2731.712 kb on - strand, within Pf6N2E2_2305 at 2731.789 kb on + strand, within Pf6N2E2_2305 at 2731.790 kb on - strand, within Pf6N2E2_2305 at 2731.790 kb on - strand, within Pf6N2E2_2305 at 2731.885 kb on + strand at 2731.885 kb on + strand at 2731.885 kb on + strand at 2731.886 kb on - strand at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.138 kb on + strand, within Pf6N2E2_2306 at 2732.139 kb on - strand, within Pf6N2E2_2306 at 2732.336 kb on + strand, within Pf6N2E2_2306 at 2732.337 kb on - strand, within Pf6N2E2_2306 at 2732.594 kb on - strand at 2732.594 kb on - strand at 2732.662 kb on - strand, within Pf6N2E2_2307 at 2732.793 kb on + strand at 2732.793 kb on + strand at 2732.793 kb on + strand at 2732.794 kb on - strand at 2732.924 kb on - strand, within Pf6N2E2_2308 at 2732.924 kb on - strand, within Pf6N2E2_2308 at 2733.027 kb on + strand, within Pf6N2E2_2308 at 2733.350 kb on + strand, within Pf6N2E2_2308 at 2733.385 kb on + strand, within Pf6N2E2_2308 at 2733.386 kb on - strand, within Pf6N2E2_2308 at 2733.386 kb on - strand, within Pf6N2E2_2308 at 2733.390 kb on - strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.553 kb on + strand, within Pf6N2E2_2308 at 2733.554 kb on - strand, within Pf6N2E2_2308 at 2733.609 kb on + strand, within Pf6N2E2_2308 at 2733.610 kb on - strand, within Pf6N2E2_2308 at 2733.610 kb on - strand, within Pf6N2E2_2308 at 2733.633 kb on - strand, within Pf6N2E2_2308 at 2733.664 kb on + strand at 2733.665 kb on - strand at 2733.670 kb on + strand at 2733.671 kb on - strand at 2733.730 kb on + strand at 2733.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,731,615 + Pf6N2E2_2305 0.76 -2.1 2,731,616 - Pf6N2E2_2305 0.76 -1.4 2,731,712 - Pf6N2E2_2305 0.83 -1.3 2,731,789 + Pf6N2E2_2305 0.88 -1.4 2,731,790 - Pf6N2E2_2305 0.88 -1.0 2,731,790 - Pf6N2E2_2305 0.88 -1.7 2,731,885 + -2.6 2,731,885 + -2.1 2,731,885 + +0.4 2,731,886 - -0.6 2,732,138 + Pf6N2E2_2306 0.29 +0.6 2,732,138 + Pf6N2E2_2306 0.29 -0.1 2,732,138 + Pf6N2E2_2306 0.29 -1.7 2,732,138 + Pf6N2E2_2306 0.29 -1.7 2,732,139 - Pf6N2E2_2306 0.29 +1.4 2,732,336 + Pf6N2E2_2306 0.61 -1.4 2,732,337 - Pf6N2E2_2306 0.61 -0.1 2,732,594 - -0.1 2,732,594 - +0.3 2,732,662 - Pf6N2E2_2307 0.34 +0.6 2,732,793 + -2.0 2,732,793 + -0.1 2,732,793 + +0.5 2,732,794 - +0.4 2,732,924 - Pf6N2E2_2308 0.15 -1.5 2,732,924 - Pf6N2E2_2308 0.15 +1.2 2,733,027 + Pf6N2E2_2308 0.26 -3.0 2,733,350 + Pf6N2E2_2308 0.60 +0.0 2,733,385 + Pf6N2E2_2308 0.64 -2.2 2,733,386 - Pf6N2E2_2308 0.64 -0.3 2,733,386 - Pf6N2E2_2308 0.64 -0.8 2,733,390 - Pf6N2E2_2308 0.64 -2.3 2,733,553 + Pf6N2E2_2308 0.81 -2.2 2,733,553 + Pf6N2E2_2308 0.81 -0.5 2,733,553 + Pf6N2E2_2308 0.81 +0.8 2,733,554 - Pf6N2E2_2308 0.81 -0.9 2,733,609 + Pf6N2E2_2308 0.87 -1.4 2,733,610 - Pf6N2E2_2308 0.87 -1.3 2,733,610 - Pf6N2E2_2308 0.87 -0.9 2,733,633 - Pf6N2E2_2308 0.90 +0.2 2,733,664 + -0.8 2,733,665 - -0.9 2,733,670 + -0.9 2,733,671 - -1.3 2,733,730 + -1.8 2,733,731 - -0.8
Or see this region's nucleotide sequence