Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2214 and Pf6N2E2_2215 are separated by 224 nucleotides Pf6N2E2_2215 and Pf6N2E2_2216 are separated by 148 nucleotides Pf6N2E2_2216 and Pf6N2E2_2217 are separated by 161 nucleotides
Pf6N2E2_2214 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 2,623,221 to 2,625,092
_2214
Pf6N2E2_2215 - DNA-binding protein HU-beta, at 2,625,317 to 2,625,589
_2215
Pf6N2E2_2216 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 2,625,738 to 2,628,134
_2216
Pf6N2E2_2217 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 2,628,296 to 2,629,579
_2217
Position (kb)
2625
2626
2627
2628
2629 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8 at 2625.192 kb on - strand at 2625.259 kb on - strand at 2625.689 kb on - strand at 2625.689 kb on - strand at 2626.765 kb on + strand, within Pf6N2E2_2216 at 2628.180 kb on - strand at 2628.180 kb on - strand at 2628.180 kb on - strand at 2628.180 kb on - strand at 2628.180 kb on - strand at 2628.237 kb on - strand at 2628.298 kb on - strand at 2628.298 kb on - strand at 2628.298 kb on - strand at 2628.340 kb on - strand at 2628.340 kb on - strand at 2628.340 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.344 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.346 kb on - strand at 2628.369 kb on - strand at 2628.369 kb on - strand at 2628.395 kb on - strand at 2628.424 kb on - strand at 2628.424 kb on - strand at 2628.441 kb on - strand, within Pf6N2E2_2217 at 2628.540 kb on - strand, within Pf6N2E2_2217 at 2628.558 kb on - strand, within Pf6N2E2_2217 at 2628.568 kb on - strand, within Pf6N2E2_2217 at 2628.568 kb on - strand, within Pf6N2E2_2217 at 2628.568 kb on - strand, within Pf6N2E2_2217 at 2628.568 kb on - strand, within Pf6N2E2_2217 at 2629.030 kb on - strand, within Pf6N2E2_2217 at 2629.030 kb on - strand, within Pf6N2E2_2217 at 2629.063 kb on - strand, within Pf6N2E2_2217 at 2629.063 kb on - strand, within Pf6N2E2_2217
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,625,192 - -0.6 2,625,259 - -1.1 2,625,689 - -1.6 2,625,689 - +0.6 2,626,765 + Pf6N2E2_2216 0.43 +0.0 2,628,180 - -1.0 2,628,180 - -1.6 2,628,180 - +0.1 2,628,180 - +0.7 2,628,180 - -1.3 2,628,237 - +0.5 2,628,298 - +3.2 2,628,298 - +3.0 2,628,298 - +2.7 2,628,340 - +1.8 2,628,340 - +1.2 2,628,340 - +1.1 2,628,344 - +1.5 2,628,344 - +2.1 2,628,344 - +2.5 2,628,344 - +2.6 2,628,344 - +3.0 2,628,344 - +1.6 2,628,344 - +1.9 2,628,346 - +1.7 2,628,346 - +1.8 2,628,346 - +3.4 2,628,346 - +1.1 2,628,346 - +2.5 2,628,346 - +0.5 2,628,346 - -0.1 2,628,346 - +1.4 2,628,346 - +2.6 2,628,346 - +1.1 2,628,346 - +1.4 2,628,346 - +0.5 2,628,346 - -0.7 2,628,346 - +2.2 2,628,346 - +3.1 2,628,346 - +2.7 2,628,346 - +2.1 2,628,346 - +1.2 2,628,346 - +2.5 2,628,346 - +2.2 2,628,346 - +3.2 2,628,346 - +3.0 2,628,346 - +0.9 2,628,346 - +2.8 2,628,369 - +0.4 2,628,369 - +1.0 2,628,395 - +6.9 2,628,424 - +6.2 2,628,424 - +7.6 2,628,441 - Pf6N2E2_2217 0.11 +7.2 2,628,540 - Pf6N2E2_2217 0.19 +7.4 2,628,558 - Pf6N2E2_2217 0.20 +6.7 2,628,568 - Pf6N2E2_2217 0.21 +5.4 2,628,568 - Pf6N2E2_2217 0.21 +6.7 2,628,568 - Pf6N2E2_2217 0.21 +8.3 2,628,568 - Pf6N2E2_2217 0.21 +6.3 2,629,030 - Pf6N2E2_2217 0.57 +6.2 2,629,030 - Pf6N2E2_2217 0.57 +7.6 2,629,063 - Pf6N2E2_2217 0.60 +6.7 2,629,063 - Pf6N2E2_2217 0.60 +6.1
Or see this region's nucleotide sequence