Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2085 and Pf6N2E2_2086 overlap by 4 nucleotides Pf6N2E2_2086 and Pf6N2E2_2087 are separated by 7 nucleotides Pf6N2E2_2087 and Pf6N2E2_2088 overlap by 1 nucleotides
Pf6N2E2_2085 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 2,478,665 to 2,481,742
_2085
Pf6N2E2_2086 - DNA polymerase-like protein PA0670, at 2,481,739 to 2,483,160
_2086
Pf6N2E2_2087 - RecA/RadA recombinase, at 2,483,168 to 2,483,800
_2087
Pf6N2E2_2088 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 2,483,800 to 2,484,417
_2088
Position (kb)
2481
2482
2483
2484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2480.767 kb on + strand, within Pf6N2E2_2085 at 2480.768 kb on - strand, within Pf6N2E2_2085 at 2480.768 kb on - strand, within Pf6N2E2_2085 at 2480.802 kb on - strand, within Pf6N2E2_2085 at 2480.814 kb on + strand, within Pf6N2E2_2085 at 2480.814 kb on + strand, within Pf6N2E2_2085 at 2480.815 kb on - strand, within Pf6N2E2_2085 at 2481.483 kb on + strand at 2481.719 kb on + strand at 2481.719 kb on + strand at 2481.719 kb on + strand at 2481.720 kb on - strand at 2481.720 kb on - strand at 2481.729 kb on + strand at 2481.729 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.833 kb on + strand at 2481.833 kb on + strand at 2481.833 kb on + strand at 2481.834 kb on - strand at 2481.834 kb on - strand at 2481.834 kb on - strand at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.570 kb on + strand, within Pf6N2E2_2086 at 2482.570 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.947 kb on - strand, within Pf6N2E2_2086 at 2483.074 kb on + strand at 2483.075 kb on - strand at 2483.145 kb on + strand at 2483.145 kb on + strand at 2483.146 kb on - strand at 2483.837 kb on - strand at 2484.065 kb on + strand, within Pf6N2E2_2088 at 2484.065 kb on + strand, within Pf6N2E2_2088
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,480,767 + Pf6N2E2_2085 0.68 +0.8 2,480,768 - Pf6N2E2_2085 0.68 +0.3 2,480,768 - Pf6N2E2_2085 0.68 -0.8 2,480,802 - Pf6N2E2_2085 0.69 +1.0 2,480,814 + Pf6N2E2_2085 0.70 -0.1 2,480,814 + Pf6N2E2_2085 0.70 -0.1 2,480,815 - Pf6N2E2_2085 0.70 -0.1 2,481,483 + -0.1 2,481,719 + -0.5 2,481,719 + -1.2 2,481,719 + +2.3 2,481,720 - +0.9 2,481,720 - +0.0 2,481,729 + +3.4 2,481,729 + -2.6 2,481,830 + +0.1 2,481,830 + +0.1 2,481,830 + -0.2 2,481,830 + -0.8 2,481,830 + -0.1 2,481,831 - +0.1 2,481,831 - +0.3 2,481,831 - -1.2 2,481,831 - +1.1 2,481,833 + -0.3 2,481,833 + -1.0 2,481,833 + -0.3 2,481,834 - +0.1 2,481,834 - +0.5 2,481,834 - +0.5 2,482,427 + Pf6N2E2_2086 0.48 +0.9 2,482,427 + Pf6N2E2_2086 0.48 -2.5 2,482,427 + Pf6N2E2_2086 0.48 -0.6 2,482,427 + Pf6N2E2_2086 0.48 -0.3 2,482,427 + Pf6N2E2_2086 0.48 +0.7 2,482,427 + Pf6N2E2_2086 0.48 +0.7 2,482,428 - Pf6N2E2_2086 0.48 +1.0 2,482,428 - Pf6N2E2_2086 0.48 -2.2 2,482,428 - Pf6N2E2_2086 0.48 -0.4 2,482,428 - Pf6N2E2_2086 0.48 +1.7 2,482,428 - Pf6N2E2_2086 0.48 -1.0 2,482,570 + Pf6N2E2_2086 0.58 +0.6 2,482,570 + Pf6N2E2_2086 0.58 +0.5 2,482,703 + Pf6N2E2_2086 0.68 -0.3 2,482,703 + Pf6N2E2_2086 0.68 -2.5 2,482,703 + Pf6N2E2_2086 0.68 +1.2 2,482,703 + Pf6N2E2_2086 0.68 +1.1 2,482,703 + Pf6N2E2_2086 0.68 +1.2 2,482,703 + Pf6N2E2_2086 0.68 +1.6 2,482,858 - Pf6N2E2_2086 0.79 +0.3 2,482,858 - Pf6N2E2_2086 0.79 +0.5 2,482,858 - Pf6N2E2_2086 0.79 -2.0 2,482,947 - Pf6N2E2_2086 0.85 +1.6 2,483,074 + -1.1 2,483,075 - -2.9 2,483,145 + +0.3 2,483,145 + +0.2 2,483,146 - +0.8 2,483,837 - -0.4 2,484,065 + Pf6N2E2_2088 0.43 -0.8 2,484,065 + Pf6N2E2_2088 0.43 +0.5
Or see this region's nucleotide sequence