Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_19 and Pf6N2E2_20 are separated by 0 nucleotides Pf6N2E2_20 and Pf6N2E2_21 are separated by 531 nucleotides
Pf6N2E2_19 - Pyoverdine efflux carrier and ATP binding protein, at 52,480 to 54,450
_19
Pf6N2E2_20 - pyoverdine-specific efflux macA-like protein, at 54,451 to 55,602
_20
Pf6N2E2_21 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis, at 56,134 to 56,616
_21
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 53.481 kb on - strand, within Pf6N2E2_19 at 54.107 kb on + strand, within Pf6N2E2_19 at 54.107 kb on + strand, within Pf6N2E2_19 at 54.161 kb on + strand, within Pf6N2E2_19 at 54.712 kb on + strand, within Pf6N2E2_20 at 54.712 kb on + strand, within Pf6N2E2_20 at 54.731 kb on + strand, within Pf6N2E2_20 at 54.732 kb on - strand, within Pf6N2E2_20 at 54.732 kb on - strand, within Pf6N2E2_20 at 54.732 kb on - strand, within Pf6N2E2_20 at 54.732 kb on - strand, within Pf6N2E2_20 at 54.872 kb on + strand, within Pf6N2E2_20 at 54.873 kb on - strand, within Pf6N2E2_20 at 55.422 kb on - strand, within Pf6N2E2_20 at 55.668 kb on - strand at 55.694 kb on - strand at 55.761 kb on + strand at 55.761 kb on + strand at 55.762 kb on - strand at 55.762 kb on - strand at 55.762 kb on - strand at 55.773 kb on + strand at 55.774 kb on - strand at 55.836 kb on + strand at 55.845 kb on + strand at 55.845 kb on + strand at 55.916 kb on + strand at 55.917 kb on - strand at 55.917 kb on - strand at 55.917 kb on - strand at 55.917 kb on - strand at 55.935 kb on + strand at 55.935 kb on + strand at 55.935 kb on + strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.936 kb on - strand at 55.944 kb on + strand at 55.944 kb on + strand at 55.945 kb on - strand at 55.997 kb on + strand at 55.998 kb on - strand at 56.021 kb on - strand at 56.029 kb on + strand at 56.104 kb on + strand at 56.104 kb on + strand at 56.146 kb on + strand at 56.147 kb on - strand at 56.147 kb on - strand at 56.224 kb on + strand, within Pf6N2E2_21 at 56.224 kb on + strand, within Pf6N2E2_21 at 56.225 kb on - strand, within Pf6N2E2_21 at 56.225 kb on - strand, within Pf6N2E2_21 at 56.284 kb on + strand, within Pf6N2E2_21 at 56.284 kb on + strand, within Pf6N2E2_21 at 56.328 kb on + strand, within Pf6N2E2_21 at 56.329 kb on - strand, within Pf6N2E2_21
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 53,481 - Pf6N2E2_19 0.51 +0.2 54,107 + Pf6N2E2_19 0.83 -0.9 54,107 + Pf6N2E2_19 0.83 -1.7 54,161 + Pf6N2E2_19 0.85 -0.6 54,712 + Pf6N2E2_20 0.23 -2.1 54,712 + Pf6N2E2_20 0.23 -0.5 54,731 + Pf6N2E2_20 0.24 -0.5 54,732 - Pf6N2E2_20 0.24 +0.6 54,732 - Pf6N2E2_20 0.24 -0.7 54,732 - Pf6N2E2_20 0.24 -1.0 54,732 - Pf6N2E2_20 0.24 +0.4 54,872 + Pf6N2E2_20 0.37 -0.6 54,873 - Pf6N2E2_20 0.37 +0.1 55,422 - Pf6N2E2_20 0.84 -0.9 55,668 - -1.4 55,694 - -2.2 55,761 + -1.7 55,761 + -0.2 55,762 - +0.5 55,762 - +0.6 55,762 - -1.2 55,773 + +0.7 55,774 - -0.5 55,836 + -1.1 55,845 + -1.5 55,845 + +0.0 55,916 + -0.4 55,917 - -2.9 55,917 - -0.9 55,917 - -0.7 55,917 - -0.1 55,935 + -0.2 55,935 + +1.2 55,935 + -0.4 55,936 - -1.1 55,936 - +0.9 55,936 - +1.1 55,936 - +0.5 55,936 - -1.6 55,936 - -1.8 55,936 - +0.3 55,944 + -0.0 55,944 + +0.7 55,945 - -2.1 55,997 + -1.4 55,998 - +0.3 56,021 - +1.9 56,029 + +1.3 56,104 + -0.1 56,104 + +0.2 56,146 + -0.7 56,147 - +0.1 56,147 - -0.8 56,224 + Pf6N2E2_21 0.19 +0.2 56,224 + Pf6N2E2_21 0.19 +1.2 56,225 - Pf6N2E2_21 0.19 +0.9 56,225 - Pf6N2E2_21 0.19 +0.9 56,284 + Pf6N2E2_21 0.31 -0.3 56,284 + Pf6N2E2_21 0.31 +0.5 56,328 + Pf6N2E2_21 0.40 -0.9 56,329 - Pf6N2E2_21 0.40 -0.8
Or see this region's nucleotide sequence