Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1827 and Pf6N2E2_1828 are separated by 26 nucleotides
Pf6N2E2_1827 - Choline-sulfatase (EC 3.1.6.6), at 2,162,920 to 2,164,599
_1827
Pf6N2E2_1828 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,164,626 to 2,166,020
_1828
Position (kb)
2162
2163
2164
2165 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2161.933 kb on - strand at 2162.112 kb on + strand at 2162.113 kb on - strand at 2162.247 kb on + strand at 2162.374 kb on + strand at 2162.375 kb on - strand at 2162.428 kb on + strand at 2162.429 kb on - strand at 2162.499 kb on + strand at 2162.499 kb on + strand at 2162.500 kb on - strand at 2162.500 kb on - strand at 2162.687 kb on - strand at 2162.690 kb on - strand at 2162.690 kb on - strand at 2162.712 kb on - strand at 2162.712 kb on - strand at 2162.712 kb on - strand at 2162.712 kb on - strand at 2162.720 kb on + strand at 2162.720 kb on + strand at 2162.720 kb on + strand at 2162.721 kb on - strand at 2162.722 kb on + strand at 2162.723 kb on - strand at 2162.723 kb on - strand at 2162.726 kb on + strand at 2162.727 kb on - strand at 2162.727 kb on - strand at 2162.788 kb on - strand at 2162.985 kb on - strand at 2162.985 kb on - strand at 2163.087 kb on + strand at 2163.173 kb on + strand, within Pf6N2E2_1827 at 2163.174 kb on - strand, within Pf6N2E2_1827 at 2163.174 kb on - strand, within Pf6N2E2_1827 at 2163.202 kb on - strand, within Pf6N2E2_1827 at 2163.202 kb on - strand, within Pf6N2E2_1827 at 2163.217 kb on + strand, within Pf6N2E2_1827 at 2163.260 kb on + strand, within Pf6N2E2_1827 at 2163.282 kb on + strand, within Pf6N2E2_1827 at 2163.283 kb on - strand, within Pf6N2E2_1827 at 2163.283 kb on - strand, within Pf6N2E2_1827 at 2163.299 kb on - strand, within Pf6N2E2_1827 at 2163.299 kb on - strand, within Pf6N2E2_1827 at 2163.408 kb on - strand, within Pf6N2E2_1827 at 2163.464 kb on + strand, within Pf6N2E2_1827 at 2163.559 kb on - strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.634 kb on - strand, within Pf6N2E2_1827 at 2163.702 kb on - strand, within Pf6N2E2_1827 at 2163.765 kb on + strand, within Pf6N2E2_1827 at 2163.765 kb on + strand, within Pf6N2E2_1827 at 2163.766 kb on - strand, within Pf6N2E2_1827 at 2163.843 kb on - strand, within Pf6N2E2_1827 at 2163.890 kb on - strand, within Pf6N2E2_1827 at 2163.957 kb on - strand, within Pf6N2E2_1827 at 2163.975 kb on + strand, within Pf6N2E2_1827 at 2164.013 kb on - strand, within Pf6N2E2_1827 at 2164.034 kb on + strand, within Pf6N2E2_1827 at 2164.068 kb on + strand, within Pf6N2E2_1827 at 2164.068 kb on + strand, within Pf6N2E2_1827 at 2164.115 kb on + strand, within Pf6N2E2_1827 at 2164.116 kb on - strand, within Pf6N2E2_1827 at 2164.123 kb on - strand, within Pf6N2E2_1827 at 2164.389 kb on - strand, within Pf6N2E2_1827 at 2164.407 kb on + strand, within Pf6N2E2_1827 at 2164.408 kb on - strand, within Pf6N2E2_1827 at 2164.466 kb on - strand at 2164.648 kb on - strand at 2164.648 kb on - strand at 2164.719 kb on - strand at 2164.720 kb on + strand at 2164.720 kb on + strand at 2164.721 kb on - strand at 2164.774 kb on + strand, within Pf6N2E2_1828 at 2164.835 kb on - strand, within Pf6N2E2_1828 at 2164.925 kb on - strand, within Pf6N2E2_1828 at 2164.925 kb on - strand, within Pf6N2E2_1828 at 2164.928 kb on + strand, within Pf6N2E2_1828 at 2164.929 kb on - strand, within Pf6N2E2_1828 at 2164.973 kb on - strand, within Pf6N2E2_1828 at 2164.993 kb on + strand, within Pf6N2E2_1828 at 2164.994 kb on - strand, within Pf6N2E2_1828 at 2164.994 kb on - strand, within Pf6N2E2_1828 at 2165.128 kb on - strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.261 kb on + strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.287 kb on - strand, within Pf6N2E2_1828 at 2165.358 kb on + strand, within Pf6N2E2_1828 at 2165.391 kb on - strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.423 kb on - strand, within Pf6N2E2_1828 at 2165.423 kb on - strand, within Pf6N2E2_1828 at 2165.473 kb on - strand, within Pf6N2E2_1828 at 2165.532 kb on + strand, within Pf6N2E2_1828 at 2165.532 kb on + strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.591 kb on - strand, within Pf6N2E2_1828 at 2165.591 kb on - strand, within Pf6N2E2_1828
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 2,161,933 - +0.2 2,162,112 + +0.0 2,162,113 - +0.9 2,162,247 + +0.6 2,162,374 + -0.4 2,162,375 - +0.8 2,162,428 + +0.1 2,162,429 - +0.9 2,162,499 + -1.1 2,162,499 + +1.5 2,162,500 - +1.6 2,162,500 - -0.1 2,162,687 - +1.7 2,162,690 - -0.9 2,162,690 - +1.1 2,162,712 - +1.5 2,162,712 - -0.3 2,162,712 - -1.0 2,162,712 - +0.1 2,162,720 + -0.5 2,162,720 + +0.5 2,162,720 + -1.4 2,162,721 - +3.7 2,162,722 + -0.5 2,162,723 - +0.0 2,162,723 - -0.5 2,162,726 + -0.2 2,162,727 - +1.2 2,162,727 - -1.3 2,162,788 - +1.3 2,162,985 - -1.5 2,162,985 - -1.1 2,163,087 + +0.6 2,163,173 + Pf6N2E2_1827 0.15 +0.3 2,163,174 - Pf6N2E2_1827 0.15 +0.4 2,163,174 - Pf6N2E2_1827 0.15 -1.4 2,163,202 - Pf6N2E2_1827 0.17 -1.4 2,163,202 - Pf6N2E2_1827 0.17 -1.3 2,163,217 + Pf6N2E2_1827 0.18 -1.8 2,163,260 + Pf6N2E2_1827 0.20 +0.2 2,163,282 + Pf6N2E2_1827 0.22 -1.3 2,163,283 - Pf6N2E2_1827 0.22 -1.4 2,163,283 - Pf6N2E2_1827 0.22 -0.5 2,163,299 - Pf6N2E2_1827 0.23 -0.3 2,163,299 - Pf6N2E2_1827 0.23 -0.5 2,163,408 - Pf6N2E2_1827 0.29 -0.2 2,163,464 + Pf6N2E2_1827 0.32 +0.7 2,163,559 - Pf6N2E2_1827 0.38 +0.0 2,163,633 + Pf6N2E2_1827 0.42 +0.3 2,163,633 + Pf6N2E2_1827 0.42 +0.3 2,163,633 + Pf6N2E2_1827 0.42 +0.7 2,163,633 + Pf6N2E2_1827 0.42 +1.6 2,163,634 - Pf6N2E2_1827 0.42 -0.4 2,163,702 - Pf6N2E2_1827 0.47 -0.9 2,163,765 + Pf6N2E2_1827 0.50 +1.2 2,163,765 + Pf6N2E2_1827 0.50 -0.6 2,163,766 - Pf6N2E2_1827 0.50 -1.5 2,163,843 - Pf6N2E2_1827 0.55 -2.1 2,163,890 - Pf6N2E2_1827 0.58 +0.5 2,163,957 - Pf6N2E2_1827 0.62 +0.4 2,163,975 + Pf6N2E2_1827 0.63 -0.3 2,164,013 - Pf6N2E2_1827 0.65 +0.6 2,164,034 + Pf6N2E2_1827 0.66 -1.4 2,164,068 + Pf6N2E2_1827 0.68 -0.3 2,164,068 + Pf6N2E2_1827 0.68 -0.1 2,164,115 + Pf6N2E2_1827 0.71 -0.7 2,164,116 - Pf6N2E2_1827 0.71 +0.2 2,164,123 - Pf6N2E2_1827 0.72 -1.2 2,164,389 - Pf6N2E2_1827 0.87 +0.2 2,164,407 + Pf6N2E2_1827 0.89 +0.5 2,164,408 - Pf6N2E2_1827 0.89 +1.0 2,164,466 - -0.7 2,164,648 - -2.5 2,164,648 - -2.3 2,164,719 - -2.1 2,164,720 + +1.0 2,164,720 + -0.2 2,164,721 - -0.6 2,164,774 + Pf6N2E2_1828 0.11 -1.2 2,164,835 - Pf6N2E2_1828 0.15 +0.6 2,164,925 - Pf6N2E2_1828 0.21 -0.9 2,164,925 - Pf6N2E2_1828 0.21 -0.5 2,164,928 + Pf6N2E2_1828 0.22 -0.6 2,164,929 - Pf6N2E2_1828 0.22 -0.5 2,164,973 - Pf6N2E2_1828 0.25 -1.0 2,164,993 + Pf6N2E2_1828 0.26 +1.3 2,164,994 - Pf6N2E2_1828 0.26 +1.8 2,164,994 - Pf6N2E2_1828 0.26 -1.0 2,165,128 - Pf6N2E2_1828 0.36 +0.6 2,165,241 + Pf6N2E2_1828 0.44 +1.3 2,165,241 + Pf6N2E2_1828 0.44 +0.5 2,165,241 + Pf6N2E2_1828 0.44 +0.9 2,165,242 - Pf6N2E2_1828 0.44 -0.2 2,165,242 - Pf6N2E2_1828 0.44 -0.2 2,165,242 - Pf6N2E2_1828 0.44 -1.6 2,165,242 - Pf6N2E2_1828 0.44 -0.1 2,165,245 + Pf6N2E2_1828 0.44 +3.0 2,165,245 + Pf6N2E2_1828 0.44 -1.9 2,165,245 + Pf6N2E2_1828 0.44 -0.6 2,165,246 - Pf6N2E2_1828 0.44 -0.3 2,165,246 - Pf6N2E2_1828 0.44 -0.0 2,165,246 - Pf6N2E2_1828 0.44 -0.6 2,165,261 + Pf6N2E2_1828 0.46 +0.5 2,165,262 - Pf6N2E2_1828 0.46 +0.7 2,165,262 - Pf6N2E2_1828 0.46 -0.7 2,165,262 - Pf6N2E2_1828 0.46 -0.2 2,165,287 - Pf6N2E2_1828 0.47 +0.4 2,165,358 + Pf6N2E2_1828 0.52 +0.5 2,165,391 - Pf6N2E2_1828 0.55 +0.9 2,165,419 + Pf6N2E2_1828 0.57 -0.3 2,165,419 + Pf6N2E2_1828 0.57 +0.5 2,165,419 + Pf6N2E2_1828 0.57 -0.3 2,165,423 - Pf6N2E2_1828 0.57 +0.2 2,165,423 - Pf6N2E2_1828 0.57 +2.2 2,165,473 - Pf6N2E2_1828 0.61 +0.2 2,165,532 + Pf6N2E2_1828 0.65 -0.6 2,165,532 + Pf6N2E2_1828 0.65 +0.2 2,165,533 - Pf6N2E2_1828 0.65 -0.8 2,165,533 - Pf6N2E2_1828 0.65 +3.2 2,165,533 - Pf6N2E2_1828 0.65 -0.2 2,165,533 - Pf6N2E2_1828 0.65 -0.4 2,165,591 - Pf6N2E2_1828 0.69 +0.3 2,165,591 - Pf6N2E2_1828 0.69 -0.8
Or see this region's nucleotide sequence