Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1594 and Pf6N2E2_1595 are separated by 196 nucleotides Pf6N2E2_1595 and Pf6N2E2_1596 are separated by 37 nucleotides Pf6N2E2_1596 and Pf6N2E2_1597 overlap by 4 nucleotides Pf6N2E2_1597 and Pf6N2E2_1598 are separated by 11 nucleotides Pf6N2E2_1598 and Pf6N2E2_1599 are separated by 9 nucleotides
Pf6N2E2_1594 - Tia invasion determinant-related protein precursor, at 1,867,125 to 1,867,823
_1594
Pf6N2E2_1595 - Periplasmic nitrate reductase component NapE, at 1,868,020 to 1,868,187
_1595
Pf6N2E2_1596 - Periplasmic nitrate reductase component NapD, at 1,868,225 to 1,868,485
_1596
Pf6N2E2_1597 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 1,868,482 to 1,870,986
_1597
Pf6N2E2_1598 - Nitrate reductase cytochrome c550-type subunit, at 1,870,998 to 1,871,483
_1598
Pf6N2E2_1599 - Cytochrome c-type protein NapC, at 1,871,493 to 1,872,089
_1599
Position (kb)
1868
1869
1870
1871 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1867.500 kb on + strand, within Pf6N2E2_1594 at 1867.501 kb on - strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.545 kb on + strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.546 kb on - strand, within Pf6N2E2_1594 at 1867.711 kb on - strand, within Pf6N2E2_1594 at 1867.779 kb on + strand at 1867.812 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.821 kb on + strand at 1867.952 kb on + strand at 1867.953 kb on - strand at 1867.991 kb on - strand at 1868.162 kb on - strand, within Pf6N2E2_1595 at 1868.162 kb on - strand, within Pf6N2E2_1595 at 1868.211 kb on - strand at 1868.243 kb on - strand at 1868.243 kb on - strand at 1868.243 kb on - strand at 1868.259 kb on + strand, within Pf6N2E2_1596 at 1868.260 kb on - strand, within Pf6N2E2_1596 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.761 kb on + strand, within Pf6N2E2_1597 at 1868.762 kb on - strand, within Pf6N2E2_1597 at 1868.762 kb on - strand, within Pf6N2E2_1597 at 1869.116 kb on + strand, within Pf6N2E2_1597 at 1869.117 kb on - strand, within Pf6N2E2_1597 at 1869.204 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.296 kb on - strand, within Pf6N2E2_1597 at 1869.565 kb on + strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.653 kb on - strand, within Pf6N2E2_1597 at 1869.736 kb on - strand, within Pf6N2E2_1597 at 1869.937 kb on - strand, within Pf6N2E2_1597 at 1870.021 kb on + strand, within Pf6N2E2_1597 at 1870.254 kb on + strand, within Pf6N2E2_1597 at 1870.328 kb on - strand, within Pf6N2E2_1597 at 1870.387 kb on + strand, within Pf6N2E2_1597 at 1870.388 kb on - strand, within Pf6N2E2_1597 at 1870.520 kb on - strand, within Pf6N2E2_1597 at 1870.549 kb on + strand, within Pf6N2E2_1597 at 1870.624 kb on + strand, within Pf6N2E2_1597 at 1870.729 kb on + strand, within Pf6N2E2_1597 at 1870.865 kb on + strand at 1870.866 kb on - strand at 1871.184 kb on + strand, within Pf6N2E2_1598 at 1871.319 kb on + strand, within Pf6N2E2_1598 at 1871.320 kb on - strand, within Pf6N2E2_1598 at 1871.368 kb on - strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.404 kb on + strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.405 kb on - strand, within Pf6N2E2_1598 at 1871.524 kb on - strand at 1871.553 kb on + strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.554 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.557 kb on - strand, within Pf6N2E2_1599 at 1871.715 kb on + strand, within Pf6N2E2_1599 at 1871.781 kb on - strand, within Pf6N2E2_1599
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,867,500 + Pf6N2E2_1594 0.54 -0.8 1,867,501 - Pf6N2E2_1594 0.54 +0.1 1,867,545 + Pf6N2E2_1594 0.60 +0.7 1,867,545 + Pf6N2E2_1594 0.60 -0.6 1,867,545 + Pf6N2E2_1594 0.60 +0.8 1,867,545 + Pf6N2E2_1594 0.60 +1.1 1,867,545 + Pf6N2E2_1594 0.60 +1.0 1,867,545 + Pf6N2E2_1594 0.60 -1.2 1,867,546 - Pf6N2E2_1594 0.60 +0.9 1,867,546 - Pf6N2E2_1594 0.60 -1.2 1,867,546 - Pf6N2E2_1594 0.60 +2.1 1,867,711 - Pf6N2E2_1594 0.84 +0.7 1,867,779 + +0.6 1,867,812 + -1.9 1,867,821 + +0.5 1,867,821 + +0.5 1,867,821 + -3.0 1,867,821 + +1.2 1,867,821 + -0.2 1,867,952 + +1.2 1,867,953 - -2.9 1,867,991 - +1.1 1,868,162 - Pf6N2E2_1595 0.85 +1.8 1,868,162 - Pf6N2E2_1595 0.85 +0.8 1,868,211 - +0.4 1,868,243 - -0.5 1,868,243 - +1.7 1,868,243 - +0.1 1,868,259 + Pf6N2E2_1596 0.13 -1.5 1,868,260 - Pf6N2E2_1596 0.13 -0.5 1,868,761 + Pf6N2E2_1597 0.11 -0.4 1,868,761 + Pf6N2E2_1597 0.11 +0.1 1,868,761 + Pf6N2E2_1597 0.11 -0.6 1,868,761 + Pf6N2E2_1597 0.11 -0.2 1,868,762 - Pf6N2E2_1597 0.11 +0.3 1,868,762 - Pf6N2E2_1597 0.11 -0.0 1,869,116 + Pf6N2E2_1597 0.25 +0.1 1,869,117 - Pf6N2E2_1597 0.25 +1.0 1,869,204 - Pf6N2E2_1597 0.29 +1.3 1,869,296 - Pf6N2E2_1597 0.32 -0.9 1,869,296 - Pf6N2E2_1597 0.32 +0.3 1,869,296 - Pf6N2E2_1597 0.32 +0.5 1,869,296 - Pf6N2E2_1597 0.32 +0.2 1,869,565 + Pf6N2E2_1597 0.43 +1.2 1,869,653 - Pf6N2E2_1597 0.47 -1.4 1,869,653 - Pf6N2E2_1597 0.47 -1.2 1,869,653 - Pf6N2E2_1597 0.47 +1.6 1,869,653 - Pf6N2E2_1597 0.47 -0.2 1,869,736 - Pf6N2E2_1597 0.50 -0.3 1,869,937 - Pf6N2E2_1597 0.58 -0.2 1,870,021 + Pf6N2E2_1597 0.61 +0.7 1,870,254 + Pf6N2E2_1597 0.71 +0.9 1,870,328 - Pf6N2E2_1597 0.74 +0.2 1,870,387 + Pf6N2E2_1597 0.76 -0.9 1,870,388 - Pf6N2E2_1597 0.76 -0.0 1,870,520 - Pf6N2E2_1597 0.81 -0.8 1,870,549 + Pf6N2E2_1597 0.83 -1.8 1,870,624 + Pf6N2E2_1597 0.86 +0.5 1,870,729 + Pf6N2E2_1597 0.90 -0.2 1,870,865 + +1.1 1,870,866 - -0.1 1,871,184 + Pf6N2E2_1598 0.38 -0.4 1,871,319 + Pf6N2E2_1598 0.66 +0.3 1,871,320 - Pf6N2E2_1598 0.66 +1.0 1,871,368 - Pf6N2E2_1598 0.76 -0.3 1,871,404 + Pf6N2E2_1598 0.84 +0.2 1,871,404 + Pf6N2E2_1598 0.84 +1.1 1,871,405 - Pf6N2E2_1598 0.84 -0.5 1,871,405 - Pf6N2E2_1598 0.84 -1.6 1,871,524 - +0.9 1,871,553 + Pf6N2E2_1599 0.10 +0.4 1,871,554 - Pf6N2E2_1599 0.10 +0.9 1,871,554 - Pf6N2E2_1599 0.10 -1.9 1,871,554 - Pf6N2E2_1599 0.10 +0.8 1,871,554 - Pf6N2E2_1599 0.10 +1.5 1,871,554 - Pf6N2E2_1599 0.10 +0.1 1,871,554 - Pf6N2E2_1599 0.10 +0.2 1,871,554 - Pf6N2E2_1599 0.10 -0.1 1,871,557 - Pf6N2E2_1599 0.11 +0.1 1,871,557 - Pf6N2E2_1599 0.11 -0.3 1,871,715 + Pf6N2E2_1599 0.37 -2.7 1,871,781 - Pf6N2E2_1599 0.48 -0.1
Or see this region's nucleotide sequence