Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_158

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_156 and Pf6N2E2_157 are separated by 247 nucleotidesPf6N2E2_157 and Pf6N2E2_158 are separated by 63 nucleotidesPf6N2E2_158 and Pf6N2E2_159 are separated by 5 nucleotidesPf6N2E2_159 and Pf6N2E2_160 are separated by 248 nucleotides Pf6N2E2_156 - Carbon storage regulator, at 211,638 to 211,832 _156 Pf6N2E2_157 - hypothetical protein, at 212,080 to 212,445 _157 Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198 _158 Pf6N2E2_159 - DNA damage resistance protein (from data), at 213,204 to 213,452 _159 Pf6N2E2_160 - hypothetical protein, at 213,701 to 214,147 _160 Position (kb) 212 213 214Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 211.518 kb on + strandat 211.518 kb on + strandat 211.519 kb on - strandat 211.567 kb on + strandat 211.683 kb on - strand, within Pf6N2E2_156at 211.797 kb on + strand, within Pf6N2E2_156at 211.797 kb on + strand, within Pf6N2E2_156at 211.848 kb on + strandat 211.848 kb on + strandat 211.848 kb on + strandat 211.849 kb on - strandat 211.849 kb on - strandat 211.849 kb on - strandat 211.849 kb on - strandat 211.853 kb on + strandat 211.853 kb on + strandat 211.853 kb on + strandat 211.854 kb on - strandat 211.854 kb on - strandat 211.854 kb on - strandat 211.855 kb on + strandat 211.855 kb on + strandat 211.856 kb on - strandat 211.896 kb on - strandat 211.896 kb on - strandat 211.897 kb on + strandat 211.897 kb on + strandat 211.897 kb on + strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.898 kb on - strandat 211.903 kb on - strandat 211.903 kb on - strandat 211.908 kb on + strandat 211.908 kb on + strandat 211.909 kb on - strandat 211.909 kb on - strandat 211.909 kb on - strandat 211.909 kb on - strandat 211.909 kb on - strandat 211.909 kb on - strandat 211.921 kb on - strandat 211.921 kb on - strandat 211.955 kb on + strandat 211.955 kb on + strandat 211.975 kb on + strandat 211.976 kb on - strandat 211.976 kb on - strandat 211.976 kb on - strandat 211.976 kb on - strandat 212.044 kb on + strandat 212.045 kb on - strandat 212.108 kb on + strandat 212.634 kb on - strand, within Pf6N2E2_158at 212.670 kb on - strand, within Pf6N2E2_158at 212.684 kb on + strand, within Pf6N2E2_158at 212.684 kb on + strand, within Pf6N2E2_158at 212.685 kb on - strand, within Pf6N2E2_158at 212.685 kb on - strand, within Pf6N2E2_158at 212.685 kb on - strand, within Pf6N2E2_158at 212.690 kb on + strand, within Pf6N2E2_158at 212.690 kb on + strand, within Pf6N2E2_158at 212.690 kb on + strand, within Pf6N2E2_158at 212.690 kb on + strand, within Pf6N2E2_158at 212.690 kb on + strand, within Pf6N2E2_158at 212.691 kb on - strand, within Pf6N2E2_158at 212.691 kb on - strand, within Pf6N2E2_158at 212.882 kb on + strand, within Pf6N2E2_158at 212.882 kb on + strand, within Pf6N2E2_158at 212.883 kb on - strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 213.013 kb on + strand, within Pf6N2E2_158at 213.014 kb on - strand, within Pf6N2E2_158at 213.014 kb on - strand, within Pf6N2E2_158at 213.017 kb on + strand, within Pf6N2E2_158at 213.018 kb on - strand, within Pf6N2E2_158at 213.018 kb on - strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.058 kb on - strand, within Pf6N2E2_158at 213.058 kb on - strand, within Pf6N2E2_158at 213.068 kb on + strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.092 kb on + strand, within Pf6N2E2_158at 213.092 kb on + strand, within Pf6N2E2_158at 213.411 kb on - strand, within Pf6N2E2_159at 213.411 kb on - strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.413 kb on - strand, within Pf6N2E2_159at 213.413 kb on - strand, within Pf6N2E2_159at 213.420 kb on + strand, within Pf6N2E2_159at 213.421 kb on - strand, within Pf6N2E2_159at 213.428 kb on + strandat 213.580 kb on + strandat 213.592 kb on + strandat 213.592 kb on + strandat 213.593 kb on - strandat 213.995 kb on + strand, within Pf6N2E2_160at 213.996 kb on - strand, within Pf6N2E2_160at 214.069 kb on - strand, within Pf6N2E2_160at 214.135 kb on + strandat 214.135 kb on + strandat 214.135 kb on + strandat 214.135 kb on + strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.197 kb on + strandat 214.198 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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211,518 + -0.3
211,518 + +0.6
211,519 - +0.3
211,567 + -0.2
211,683 - Pf6N2E2_156 0.23 -1.2
211,797 + Pf6N2E2_156 0.82 -1.6
211,797 + Pf6N2E2_156 0.82 -1.0
211,848 + -1.9
211,848 + -0.8
211,848 + -0.4
211,849 - -0.4
211,849 - +1.4
211,849 - +1.3
211,849 - +0.7
211,853 + -2.1
211,853 + -2.2
211,853 + -2.0
211,854 - -2.0
211,854 - +5.7
211,854 - +0.7
211,855 + -1.6
211,855 + -0.8
211,856 - +0.7
211,896 - +1.1
211,896 - +2.5
211,897 + -0.8
211,897 + -0.6
211,897 + -1.7
211,898 - -0.3
211,898 - +1.5
211,898 - +2.0
211,898 - +0.6
211,898 - +1.0
211,898 - +2.4
211,898 - +0.2
211,898 - +1.3
211,903 - +0.7
211,903 - +0.5
211,908 + -2.9
211,908 + -0.9
211,909 - +1.3
211,909 - +0.3
211,909 - +2.6
211,909 - +0.7
211,909 - +2.1
211,909 - +2.6
211,921 - +0.4
211,921 - +2.0
211,955 + -1.0
211,955 + -0.8
211,975 + +0.7
211,976 - +0.8
211,976 - +2.0
211,976 - +0.6
211,976 - +0.4
212,044 + -1.0
212,045 - +0.6
212,108 + -1.3
212,634 - Pf6N2E2_158 0.18 -1.8
212,670 - Pf6N2E2_158 0.23 +0.2
212,684 + Pf6N2E2_158 0.25 -0.0
212,684 + Pf6N2E2_158 0.25 -0.9
212,685 - Pf6N2E2_158 0.26 -0.1
212,685 - Pf6N2E2_158 0.26 -0.9
212,685 - Pf6N2E2_158 0.26 +1.8
212,690 + Pf6N2E2_158 0.26 +0.2
212,690 + Pf6N2E2_158 0.26 -1.0
212,690 + Pf6N2E2_158 0.26 -1.4
212,690 + Pf6N2E2_158 0.26 +1.4
212,690 + Pf6N2E2_158 0.26 +0.3
212,691 - Pf6N2E2_158 0.26 +1.4
212,691 - Pf6N2E2_158 0.26 -1.7
212,882 + Pf6N2E2_158 0.54 +0.6
212,882 + Pf6N2E2_158 0.54 -1.7
212,883 - Pf6N2E2_158 0.54 +1.2
212,967 + Pf6N2E2_158 0.66 -0.6
212,967 + Pf6N2E2_158 0.66 -1.9
212,967 + Pf6N2E2_158 0.66 -1.1
212,967 + Pf6N2E2_158 0.66 +0.1
212,967 + Pf6N2E2_158 0.66 +1.2
212,967 + Pf6N2E2_158 0.66 +1.1
212,968 - Pf6N2E2_158 0.67 -1.0
212,968 - Pf6N2E2_158 0.67 +1.9
212,968 - Pf6N2E2_158 0.67 +1.3
212,968 - Pf6N2E2_158 0.67 -1.1
212,968 - Pf6N2E2_158 0.67 -1.4
213,013 + Pf6N2E2_158 0.73 -0.4
213,014 - Pf6N2E2_158 0.73 -2.0
213,014 - Pf6N2E2_158 0.73 -1.5
213,017 + Pf6N2E2_158 0.74 -0.5
213,018 - Pf6N2E2_158 0.74 +0.4
213,018 - Pf6N2E2_158 0.74 +0.5
213,053 + Pf6N2E2_158 0.79 -1.0
213,053 + Pf6N2E2_158 0.79 -1.4
213,053 + Pf6N2E2_158 0.79 -2.9
213,058 - Pf6N2E2_158 0.80 -1.0
213,058 - Pf6N2E2_158 0.80 +0.1
213,068 + Pf6N2E2_158 0.81 +0.8
213,069 - Pf6N2E2_158 0.81 -0.6
213,069 - Pf6N2E2_158 0.81 -0.4
213,069 - Pf6N2E2_158 0.81 -0.3
213,069 - Pf6N2E2_158 0.81 +0.3
213,092 + Pf6N2E2_158 0.84 -0.2
213,092 + Pf6N2E2_158 0.84 -1.1
213,411 - Pf6N2E2_159 0.83 -0.2
213,411 - Pf6N2E2_159 0.83 -0.5
213,412 + Pf6N2E2_159 0.84 -2.1
213,412 + Pf6N2E2_159 0.84 +0.6
213,412 + Pf6N2E2_159 0.84 +3.3
213,413 - Pf6N2E2_159 0.84 +0.9
213,413 - Pf6N2E2_159 0.84 +0.1
213,420 + Pf6N2E2_159 0.87 +0.2
213,421 - Pf6N2E2_159 0.87 -1.8
213,428 + -0.2
213,580 + -1.5
213,592 + +0.3
213,592 + +0.8
213,593 - +0.9
213,995 + Pf6N2E2_160 0.66 -2.3
213,996 - Pf6N2E2_160 0.66 +0.6
214,069 - Pf6N2E2_160 0.82 +0.5
214,135 + +0.6
214,135 + -0.8
214,135 + -1.6
214,135 + -2.2
214,136 - +1.0
214,136 - -0.9
214,136 - +0.2
214,136 - -1.0
214,136 - -0.9
214,136 - -0.6
214,136 - -0.8
214,197 + -0.5
214,198 - -1.2

Or see this region's nucleotide sequence