Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1370 and Pf6N2E2_1371 overlap by 21 nucleotides Pf6N2E2_1371 and Pf6N2E2_1372 are separated by 2 nucleotides Pf6N2E2_1372 and Pf6N2E2_1373 are separated by 150 nucleotides
Pf6N2E2_1370 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 1,607,107 to 1,608,579
_1370
Pf6N2E2_1371 - hypothetical protein, at 1,608,559 to 1,608,861
_1371
Pf6N2E2_1372 - sigma-54 dependent transcriptional regulator, at 1,608,864 to 1,610,639
_1372
Pf6N2E2_1373 - Transcriptional regulator of various polyols utilization, AraC family, at 1,610,790 to 1,611,692
_1373
Position (kb)
1608
1609
1610
1611 Strain fitness (log2 ratio)
-2
-1
0
1 at 1607.880 kb on + strand, within Pf6N2E2_1370 at 1607.901 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.152 kb on + strand, within Pf6N2E2_1370 at 1608.293 kb on + strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.294 kb on - strand, within Pf6N2E2_1370 at 1608.582 kb on - strand at 1608.631 kb on - strand, within Pf6N2E2_1371 at 1608.696 kb on - strand, within Pf6N2E2_1371 at 1608.810 kb on - strand, within Pf6N2E2_1371 at 1608.816 kb on + strand, within Pf6N2E2_1371 at 1608.817 kb on - strand, within Pf6N2E2_1371 at 1608.859 kb on + strand at 1608.860 kb on - strand at 1608.860 kb on - strand at 1609.013 kb on + strand at 1609.014 kb on - strand at 1609.201 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.290 kb on + strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.291 kb on - strand, within Pf6N2E2_1372 at 1609.347 kb on + strand, within Pf6N2E2_1372 at 1609.348 kb on - strand, within Pf6N2E2_1372 at 1609.389 kb on + strand, within Pf6N2E2_1372 at 1609.531 kb on - strand, within Pf6N2E2_1372 at 1609.857 kb on + strand, within Pf6N2E2_1372 at 1609.857 kb on + strand, within Pf6N2E2_1372 at 1609.857 kb on + strand, within Pf6N2E2_1372 at 1610.161 kb on + strand, within Pf6N2E2_1372 at 1610.161 kb on + strand, within Pf6N2E2_1372 at 1610.162 kb on - strand, within Pf6N2E2_1372 at 1610.162 kb on - strand, within Pf6N2E2_1372 at 1610.162 kb on - strand, within Pf6N2E2_1372 at 1610.162 kb on - strand, within Pf6N2E2_1372 at 1610.260 kb on - strand, within Pf6N2E2_1372 at 1610.386 kb on - strand, within Pf6N2E2_1372 at 1610.749 kb on + strand at 1610.750 kb on - strand at 1610.750 kb on - strand at 1610.819 kb on - strand at 1610.881 kb on + strand, within Pf6N2E2_1373 at 1611.088 kb on + strand, within Pf6N2E2_1373 at 1611.088 kb on + strand, within Pf6N2E2_1373 at 1611.089 kb on - strand, within Pf6N2E2_1373 at 1611.102 kb on - strand, within Pf6N2E2_1373 at 1611.142 kb on + strand, within Pf6N2E2_1373 at 1611.142 kb on + strand, within Pf6N2E2_1373 at 1611.142 kb on + strand, within Pf6N2E2_1373 at 1611.142 kb on + strand, within Pf6N2E2_1373 at 1611.142 kb on + strand, within Pf6N2E2_1373 at 1611.502 kb on + strand, within Pf6N2E2_1373 at 1611.502 kb on + strand, within Pf6N2E2_1373 at 1611.502 kb on + strand, within Pf6N2E2_1373 at 1611.503 kb on - strand, within Pf6N2E2_1373
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,607,880 + Pf6N2E2_1370 0.52 +0.0 1,607,901 + Pf6N2E2_1370 0.54 -0.9 1,608,152 + Pf6N2E2_1370 0.71 +0.2 1,608,152 + Pf6N2E2_1370 0.71 +0.0 1,608,152 + Pf6N2E2_1370 0.71 -1.7 1,608,293 + Pf6N2E2_1370 0.81 -0.9 1,608,294 - Pf6N2E2_1370 0.81 +1.0 1,608,294 - Pf6N2E2_1370 0.81 -0.3 1,608,294 - Pf6N2E2_1370 0.81 -2.0 1,608,582 - +0.3 1,608,631 - Pf6N2E2_1371 0.24 -0.2 1,608,696 - Pf6N2E2_1371 0.45 -1.5 1,608,810 - Pf6N2E2_1371 0.83 +0.0 1,608,816 + Pf6N2E2_1371 0.85 -0.3 1,608,817 - Pf6N2E2_1371 0.85 -0.4 1,608,859 + -2.6 1,608,860 - -1.7 1,608,860 - -1.9 1,609,013 + +0.8 1,609,014 - -0.4 1,609,201 + Pf6N2E2_1372 0.19 +0.4 1,609,290 + Pf6N2E2_1372 0.24 -1.1 1,609,290 + Pf6N2E2_1372 0.24 -0.3 1,609,290 + Pf6N2E2_1372 0.24 +1.0 1,609,291 - Pf6N2E2_1372 0.24 -0.4 1,609,291 - Pf6N2E2_1372 0.24 -0.4 1,609,291 - Pf6N2E2_1372 0.24 -1.4 1,609,291 - Pf6N2E2_1372 0.24 -1.8 1,609,291 - Pf6N2E2_1372 0.24 +0.2 1,609,291 - Pf6N2E2_1372 0.24 -1.4 1,609,347 + Pf6N2E2_1372 0.27 -1.9 1,609,348 - Pf6N2E2_1372 0.27 -1.8 1,609,389 + Pf6N2E2_1372 0.30 +0.9 1,609,531 - Pf6N2E2_1372 0.38 +1.4 1,609,857 + Pf6N2E2_1372 0.56 -0.8 1,609,857 + Pf6N2E2_1372 0.56 -1.3 1,609,857 + Pf6N2E2_1372 0.56 +0.6 1,610,161 + Pf6N2E2_1372 0.73 -2.0 1,610,161 + Pf6N2E2_1372 0.73 +1.1 1,610,162 - Pf6N2E2_1372 0.73 -0.7 1,610,162 - Pf6N2E2_1372 0.73 -0.0 1,610,162 - Pf6N2E2_1372 0.73 -1.1 1,610,162 - Pf6N2E2_1372 0.73 +1.0 1,610,260 - Pf6N2E2_1372 0.79 +0.6 1,610,386 - Pf6N2E2_1372 0.86 +0.8 1,610,749 + +0.4 1,610,750 - -0.2 1,610,750 - +0.6 1,610,819 - +1.0 1,610,881 + Pf6N2E2_1373 0.10 +0.9 1,611,088 + Pf6N2E2_1373 0.33 +0.5 1,611,088 + Pf6N2E2_1373 0.33 +0.3 1,611,089 - Pf6N2E2_1373 0.33 -0.5 1,611,102 - Pf6N2E2_1373 0.35 -1.0 1,611,142 + Pf6N2E2_1373 0.39 -1.7 1,611,142 + Pf6N2E2_1373 0.39 +0.5 1,611,142 + Pf6N2E2_1373 0.39 -0.2 1,611,142 + Pf6N2E2_1373 0.39 -1.1 1,611,142 + Pf6N2E2_1373 0.39 -1.9 1,611,502 + Pf6N2E2_1373 0.79 -2.2 1,611,502 + Pf6N2E2_1373 0.79 -1.1 1,611,502 + Pf6N2E2_1373 0.79 +0.7 1,611,503 - Pf6N2E2_1373 0.79 +0.8
Or see this region's nucleotide sequence