Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1350 and Pf6N2E2_1351 overlap by 4 nucleotides Pf6N2E2_1351 and Pf6N2E2_1352 are separated by 21 nucleotides Pf6N2E2_1352 and Pf6N2E2_1353 overlap by 4 nucleotides Pf6N2E2_1353 and Pf6N2E2_1354 overlap by 4 nucleotides
Pf6N2E2_1350 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), plant type, at 1,585,570 to 1,586,838
_1350
Pf6N2E2_1351 - FIG024285: Hypothetical protein, at 1,586,835 to 1,587,326
_1351
Pf6N2E2_1352 - Cation transport ATPase, at 1,587,348 to 1,587,653
_1352
Pf6N2E2_1353 - FIG002577: Putative lipoprotein precursor, at 1,587,650 to 1,588,189
_1353
Pf6N2E2_1354 - FIG026291: Hypothetical periplasmic protein, at 1,588,186 to 1,588,701
_1354
Position (kb)
1587
1588 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1586.389 kb on + strand, within Pf6N2E2_1350 at 1586.393 kb on + strand, within Pf6N2E2_1350 at 1586.393 kb on + strand, within Pf6N2E2_1350 at 1586.394 kb on - strand, within Pf6N2E2_1350 at 1586.402 kb on - strand, within Pf6N2E2_1350 at 1586.480 kb on - strand, within Pf6N2E2_1350 at 1586.725 kb on + strand at 1586.725 kb on + strand at 1586.725 kb on + strand at 1586.726 kb on - strand at 1586.726 kb on - strand at 1586.833 kb on - strand at 1586.926 kb on + strand, within Pf6N2E2_1351 at 1586.927 kb on - strand, within Pf6N2E2_1351 at 1586.927 kb on - strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.929 kb on + strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1586.930 kb on - strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.061 kb on + strand, within Pf6N2E2_1351 at 1587.062 kb on - strand, within Pf6N2E2_1351 at 1587.062 kb on - strand, within Pf6N2E2_1351 at 1587.131 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.169 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.201 kb on + strand, within Pf6N2E2_1351 at 1587.568 kb on - strand, within Pf6N2E2_1352 at 1587.568 kb on - strand, within Pf6N2E2_1352 at 1587.572 kb on - strand, within Pf6N2E2_1352 at 1587.572 kb on - strand, within Pf6N2E2_1352 at 1587.698 kb on + strand at 1587.760 kb on - strand, within Pf6N2E2_1353 at 1587.760 kb on - strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.786 kb on + strand, within Pf6N2E2_1353 at 1587.787 kb on - strand, within Pf6N2E2_1353 at 1587.951 kb on + strand, within Pf6N2E2_1353 at 1587.952 kb on - strand, within Pf6N2E2_1353 at 1588.047 kb on + strand, within Pf6N2E2_1353 at 1588.048 kb on - strand, within Pf6N2E2_1353 at 1588.048 kb on - strand, within Pf6N2E2_1353 at 1588.048 kb on - strand, within Pf6N2E2_1353 at 1588.115 kb on - strand, within Pf6N2E2_1353 at 1588.115 kb on - strand, within Pf6N2E2_1353 at 1588.338 kb on - strand, within Pf6N2E2_1354 at 1588.364 kb on + strand, within Pf6N2E2_1354 at 1588.364 kb on + strand, within Pf6N2E2_1354 at 1588.364 kb on + strand, within Pf6N2E2_1354 at 1588.365 kb on - strand, within Pf6N2E2_1354 at 1588.365 kb on - strand, within Pf6N2E2_1354 at 1588.402 kb on - strand, within Pf6N2E2_1354 at 1588.527 kb on - strand, within Pf6N2E2_1354 at 1588.610 kb on + strand, within Pf6N2E2_1354 at 1588.611 kb on - strand, within Pf6N2E2_1354
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,586,389 + Pf6N2E2_1350 0.65 +0.2 1,586,393 + Pf6N2E2_1350 0.65 -0.4 1,586,393 + Pf6N2E2_1350 0.65 +1.6 1,586,394 - Pf6N2E2_1350 0.65 -2.7 1,586,402 - Pf6N2E2_1350 0.66 +1.1 1,586,480 - Pf6N2E2_1350 0.72 -1.3 1,586,725 + -0.6 1,586,725 + +0.3 1,586,725 + -0.5 1,586,726 - -0.6 1,586,726 - -1.4 1,586,833 - +0.1 1,586,926 + Pf6N2E2_1351 0.18 -0.5 1,586,927 - Pf6N2E2_1351 0.19 +1.3 1,586,927 - Pf6N2E2_1351 0.19 -0.0 1,586,929 + Pf6N2E2_1351 0.19 -0.5 1,586,929 + Pf6N2E2_1351 0.19 -1.4 1,586,929 + Pf6N2E2_1351 0.19 -1.6 1,586,930 - Pf6N2E2_1351 0.19 -0.1 1,586,930 - Pf6N2E2_1351 0.19 -0.8 1,586,930 - Pf6N2E2_1351 0.19 +1.3 1,587,061 + Pf6N2E2_1351 0.46 -0.1 1,587,061 + Pf6N2E2_1351 0.46 +1.1 1,587,061 + Pf6N2E2_1351 0.46 +0.6 1,587,062 - Pf6N2E2_1351 0.46 +1.1 1,587,062 - Pf6N2E2_1351 0.46 +1.1 1,587,131 + Pf6N2E2_1351 0.60 -1.0 1,587,169 + Pf6N2E2_1351 0.68 -0.1 1,587,169 + Pf6N2E2_1351 0.68 +0.5 1,587,169 + Pf6N2E2_1351 0.68 -0.9 1,587,201 + Pf6N2E2_1351 0.74 +1.9 1,587,201 + Pf6N2E2_1351 0.74 -0.7 1,587,201 + Pf6N2E2_1351 0.74 -0.3 1,587,568 - Pf6N2E2_1352 0.72 -1.2 1,587,568 - Pf6N2E2_1352 0.72 -0.6 1,587,572 - Pf6N2E2_1352 0.73 -0.6 1,587,572 - Pf6N2E2_1352 0.73 -1.5 1,587,698 + +1.7 1,587,760 - Pf6N2E2_1353 0.20 -1.1 1,587,760 - Pf6N2E2_1353 0.20 -1.4 1,587,786 + Pf6N2E2_1353 0.25 +1.0 1,587,786 + Pf6N2E2_1353 0.25 +0.1 1,587,786 + Pf6N2E2_1353 0.25 +0.6 1,587,787 - Pf6N2E2_1353 0.25 -0.3 1,587,951 + Pf6N2E2_1353 0.56 -0.7 1,587,952 - Pf6N2E2_1353 0.56 -0.5 1,588,047 + Pf6N2E2_1353 0.74 +0.2 1,588,048 - Pf6N2E2_1353 0.74 +0.7 1,588,048 - Pf6N2E2_1353 0.74 -0.1 1,588,048 - Pf6N2E2_1353 0.74 +0.0 1,588,115 - Pf6N2E2_1353 0.86 +1.6 1,588,115 - Pf6N2E2_1353 0.86 -1.6 1,588,338 - Pf6N2E2_1354 0.29 -0.9 1,588,364 + Pf6N2E2_1354 0.34 -0.9 1,588,364 + Pf6N2E2_1354 0.34 -0.3 1,588,364 + Pf6N2E2_1354 0.34 +1.2 1,588,365 - Pf6N2E2_1354 0.35 +0.2 1,588,365 - Pf6N2E2_1354 0.35 +0.5 1,588,402 - Pf6N2E2_1354 0.42 +0.4 1,588,527 - Pf6N2E2_1354 0.66 +0.6 1,588,610 + Pf6N2E2_1354 0.82 +0.7 1,588,611 - Pf6N2E2_1354 0.82 +1.4
Or see this region's nucleotide sequence