Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1306 and Pf6N2E2_1307 are separated by 186 nucleotides Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides Pf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotides
Pf6N2E2_1306 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 1,540,379 to 1,541,284
_1306
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Position (kb) 1541
1542
1543 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1540.662 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.748 kb on + strand, within Pf6N2E2_1306 at 1540.749 kb on - strand, within Pf6N2E2_1306 at 1540.851 kb on + strand, within Pf6N2E2_1306 at 1541.079 kb on + strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.080 kb on - strand, within Pf6N2E2_1306 at 1541.099 kb on + strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.100 kb on - strand, within Pf6N2E2_1306 at 1541.124 kb on + strand, within Pf6N2E2_1306 at 1541.206 kb on - strand at 1541.206 kb on - strand at 1541.390 kb on + strand at 1541.407 kb on - strand at 1541.751 kb on - strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.801 kb on + strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.802 kb on - strand, within Pf6N2E2_1307 at 1541.912 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1542.058 kb on - strand, within Pf6N2E2_1307 at 1542.112 kb on + strand at 1542.377 kb on - strand, within Pf6N2E2_1308 at 1542.421 kb on + strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.431 kb on - strand, within Pf6N2E2_1308 at 1542.545 kb on - strand, within Pf6N2E2_1308 at 1542.559 kb on + strand, within Pf6N2E2_1308 at 1542.560 kb on - strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,540,662 + Pf6N2E2_1306 0.31 -0.9 1,540,748 + Pf6N2E2_1306 0.41 -0.3 1,540,748 + Pf6N2E2_1306 0.41 -0.8 1,540,749 - Pf6N2E2_1306 0.41 +0.3 1,540,851 + Pf6N2E2_1306 0.52 +0.1 1,541,079 + Pf6N2E2_1306 0.77 +0.9 1,541,080 - Pf6N2E2_1306 0.77 -0.3 1,541,080 - Pf6N2E2_1306 0.77 +0.8 1,541,099 + Pf6N2E2_1306 0.79 -1.3 1,541,100 - Pf6N2E2_1306 0.80 +0.1 1,541,100 - Pf6N2E2_1306 0.80 +0.8 1,541,124 + Pf6N2E2_1306 0.82 -0.9 1,541,206 - +1.4 1,541,206 - -0.9 1,541,390 + +0.1 1,541,407 - +0.9 1,541,751 - Pf6N2E2_1307 0.41 -1.9 1,541,801 + Pf6N2E2_1307 0.49 +0.3 1,541,801 + Pf6N2E2_1307 0.49 +0.5 1,541,801 + Pf6N2E2_1307 0.49 -0.7 1,541,802 - Pf6N2E2_1307 0.49 -1.3 1,541,802 - Pf6N2E2_1307 0.49 -0.7 1,541,802 - Pf6N2E2_1307 0.49 +0.2 1,541,802 - Pf6N2E2_1307 0.49 -0.9 1,541,802 - Pf6N2E2_1307 0.49 +0.2 1,541,802 - Pf6N2E2_1307 0.49 -2.0 1,541,912 + Pf6N2E2_1307 0.65 -1.0 1,541,976 + Pf6N2E2_1307 0.74 -0.6 1,541,976 + Pf6N2E2_1307 0.74 -1.0 1,541,976 + Pf6N2E2_1307 0.74 -0.0 1,541,976 + Pf6N2E2_1307 0.74 +1.4 1,541,976 + Pf6N2E2_1307 0.74 -0.0 1,542,058 - Pf6N2E2_1307 0.87 +0.9 1,542,112 + -0.1 1,542,377 - Pf6N2E2_1308 0.30 +0.7 1,542,421 + Pf6N2E2_1308 0.36 +1.7 1,542,422 - Pf6N2E2_1308 0.36 +2.2 1,542,422 - Pf6N2E2_1308 0.36 -0.6 1,542,431 - Pf6N2E2_1308 0.37 +0.7 1,542,545 - Pf6N2E2_1308 0.52 +0.3 1,542,559 + Pf6N2E2_1308 0.54 -1.5 1,542,560 - Pf6N2E2_1308 0.54 +0.1 1,542,739 + Pf6N2E2_1308 0.77 -1.3 1,542,739 + Pf6N2E2_1308 0.77 +0.9 1,542,979 - -0.2
Or see this region's nucleotide sequence