Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_123

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_121 and Pf6N2E2_122 are separated by 20 nucleotidesPf6N2E2_122 and Pf6N2E2_123 are separated by 0 nucleotidesPf6N2E2_123 and Pf6N2E2_124 overlap by 14 nucleotides Pf6N2E2_121 - probable polysaccharide biosynthesis protein, at 168,810 to 169,832 _121 Pf6N2E2_122 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 169,853 to 170,497 _122 Pf6N2E2_123 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 170,498 to 171,244 _123 Pf6N2E2_124 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 171,231 to 172,310 _124 Position (kb) 170 171 172Strain fitness (log2 ratio) -2 -1 0 1 2at 169.627 kb on + strand, within Pf6N2E2_121at 169.854 kb on + strandat 169.854 kb on + strandat 169.854 kb on + strandat 169.854 kb on + strandat 169.855 kb on - strandat 169.855 kb on - strandat 169.866 kb on + strandat 169.866 kb on + strandat 169.884 kb on + strandat 169.885 kb on - strandat 169.887 kb on + strandat 169.888 kb on - strandat 170.046 kb on + strand, within Pf6N2E2_122at 170.079 kb on + strand, within Pf6N2E2_122at 170.499 kb on + strandat 170.500 kb on - strandat 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.697 kb on + strand, within Pf6N2E2_123at 170.697 kb on + strand, within Pf6N2E2_123at 170.703 kb on + strand, within Pf6N2E2_123at 170.756 kb on + strand, within Pf6N2E2_123at 170.756 kb on + strand, within Pf6N2E2_123at 170.757 kb on - strand, within Pf6N2E2_123at 170.757 kb on - strand, within Pf6N2E2_123at 170.774 kb on + strand, within Pf6N2E2_123at 170.931 kb on + strand, within Pf6N2E2_123at 170.932 kb on - strand, within Pf6N2E2_123at 171.038 kb on + strand, within Pf6N2E2_123at 171.313 kb on + strandat 171.313 kb on + strandat 171.493 kb on + strand, within Pf6N2E2_124at 171.493 kb on + strand, within Pf6N2E2_124at 171.493 kb on + strand, within Pf6N2E2_124at 171.532 kb on + strand, within Pf6N2E2_124at 171.533 kb on - strand, within Pf6N2E2_124at 171.649 kb on + strand, within Pf6N2E2_124at 171.649 kb on + strand, within Pf6N2E2_124at 171.811 kb on + strand, within Pf6N2E2_124at 171.811 kb on + strand, within Pf6N2E2_124at 171.812 kb on - strand, within Pf6N2E2_124at 171.812 kb on - strand, within Pf6N2E2_124at 171.812 kb on - strand, within Pf6N2E2_124at 171.812 kb on - strand, within Pf6N2E2_124at 171.812 kb on - strand, within Pf6N2E2_124at 171.916 kb on + strand, within Pf6N2E2_124at 171.916 kb on + strand, within Pf6N2E2_124at 171.917 kb on - strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.937 kb on + strand, within Pf6N2E2_124at 171.981 kb on - strand, within Pf6N2E2_124at 171.981 kb on - strand, within Pf6N2E2_124at 171.981 kb on - strand, within Pf6N2E2_124at 171.981 kb on - strand, within Pf6N2E2_124at 171.981 kb on - strand, within Pf6N2E2_124at 172.012 kb on + strand, within Pf6N2E2_124at 172.013 kb on - strand, within Pf6N2E2_124at 172.013 kb on - strand, within Pf6N2E2_124at 172.013 kb on - strand, within Pf6N2E2_124at 172.013 kb on - strand, within Pf6N2E2_124at 172.013 kb on - strand, within Pf6N2E2_124at 172.071 kb on + strand, within Pf6N2E2_124at 172.202 kb on - strand, within Pf6N2E2_124at 172.204 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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169,627 + Pf6N2E2_121 0.80 +0.5
169,854 + -0.1
169,854 + +0.9
169,854 + -1.2
169,854 + +1.2
169,855 - +0.8
169,855 - +0.9
169,866 + +0.6
169,866 + -0.1
169,884 + +0.5
169,885 - -0.7
169,887 + -1.2
169,888 - +0.0
170,046 + Pf6N2E2_122 0.30 +0.6
170,079 + Pf6N2E2_122 0.35 -1.1
170,499 + +1.4
170,500 - -0.4
170,583 + Pf6N2E2_123 0.11 -1.6
170,583 + Pf6N2E2_123 0.11 -0.9
170,583 + Pf6N2E2_123 0.11 -2.2
170,583 + Pf6N2E2_123 0.11 -0.4
170,583 + Pf6N2E2_123 0.11 -1.4
170,584 - Pf6N2E2_123 0.12 +1.7
170,584 - Pf6N2E2_123 0.12 -0.6
170,584 - Pf6N2E2_123 0.12 +0.4
170,664 + Pf6N2E2_123 0.22 +0.7
170,664 + Pf6N2E2_123 0.22 -0.8
170,664 + Pf6N2E2_123 0.22 -1.3
170,697 + Pf6N2E2_123 0.27 +0.3
170,697 + Pf6N2E2_123 0.27 -0.5
170,703 + Pf6N2E2_123 0.27 -1.1
170,756 + Pf6N2E2_123 0.35 -0.1
170,756 + Pf6N2E2_123 0.35 +0.3
170,757 - Pf6N2E2_123 0.35 -1.5
170,757 - Pf6N2E2_123 0.35 -0.8
170,774 + Pf6N2E2_123 0.37 -0.5
170,931 + Pf6N2E2_123 0.58 +1.3
170,932 - Pf6N2E2_123 0.58 +0.4
171,038 + Pf6N2E2_123 0.72 -0.2
171,313 + -0.8
171,313 + -0.2
171,493 + Pf6N2E2_124 0.24 +0.7
171,493 + Pf6N2E2_124 0.24 +0.4
171,493 + Pf6N2E2_124 0.24 -1.0
171,532 + Pf6N2E2_124 0.28 -0.9
171,533 - Pf6N2E2_124 0.28 -2.1
171,649 + Pf6N2E2_124 0.39 -1.7
171,649 + Pf6N2E2_124 0.39 +1.5
171,811 + Pf6N2E2_124 0.54 -1.0
171,811 + Pf6N2E2_124 0.54 +1.4
171,812 - Pf6N2E2_124 0.54 +0.3
171,812 - Pf6N2E2_124 0.54 +0.1
171,812 - Pf6N2E2_124 0.54 -0.1
171,812 - Pf6N2E2_124 0.54 -0.8
171,812 - Pf6N2E2_124 0.54 -2.4
171,916 + Pf6N2E2_124 0.63 +0.9
171,916 + Pf6N2E2_124 0.63 +0.6
171,917 - Pf6N2E2_124 0.64 +0.2
171,937 + Pf6N2E2_124 0.65 -0.7
171,937 + Pf6N2E2_124 0.65 +0.3
171,937 + Pf6N2E2_124 0.65 +1.6
171,937 + Pf6N2E2_124 0.65 +1.0
171,937 + Pf6N2E2_124 0.65 +0.1
171,937 + Pf6N2E2_124 0.65 -0.3
171,981 - Pf6N2E2_124 0.69 +0.6
171,981 - Pf6N2E2_124 0.69 -1.4
171,981 - Pf6N2E2_124 0.69 -1.8
171,981 - Pf6N2E2_124 0.69 -0.9
171,981 - Pf6N2E2_124 0.69 +2.2
172,012 + Pf6N2E2_124 0.72 +0.3
172,013 - Pf6N2E2_124 0.72 -0.5
172,013 - Pf6N2E2_124 0.72 -0.1
172,013 - Pf6N2E2_124 0.72 +0.9
172,013 - Pf6N2E2_124 0.72 +0.4
172,013 - Pf6N2E2_124 0.72 -0.2
172,071 + Pf6N2E2_124 0.78 -0.6
172,202 - Pf6N2E2_124 0.90 -0.4
172,204 + -0.1

Or see this region's nucleotide sequence