Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1020 and Pf6N2E2_1021 are separated by 397 nucleotides Pf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides
Pf6N2E2_1020 - TonB-dependent receptor, at 1,191,280 to 1,193,481
_1020
Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826
_1021
Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022
_1022
Position (kb)
1193
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1 at 1192.880 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.113 kb on + strand, within Pf6N2E2_1020 at 1193.130 kb on - strand, within Pf6N2E2_1020 at 1193.144 kb on + strand, within Pf6N2E2_1020 at 1193.145 kb on - strand, within Pf6N2E2_1020 at 1193.392 kb on - strand at 1193.628 kb on + strand at 1193.734 kb on + strand at 1193.883 kb on + strand at 1193.895 kb on - strand at 1193.895 kb on - strand at 1193.998 kb on + strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.459 kb on - strand, within Pf6N2E2_1021 at 1194.583 kb on - strand, within Pf6N2E2_1021 at 1194.628 kb on - strand, within Pf6N2E2_1021 at 1195.244 kb on + strand, within Pf6N2E2_1022 at 1195.366 kb on + strand, within Pf6N2E2_1022 at 1195.396 kb on + strand, within Pf6N2E2_1022 at 1195.733 kb on + strand, within Pf6N2E2_1022
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 1,192,880 + Pf6N2E2_1020 0.73 -0.1 1,193,057 + Pf6N2E2_1020 0.81 -1.0 1,193,057 + Pf6N2E2_1020 0.81 -0.6 1,193,057 + Pf6N2E2_1020 0.81 +0.7 1,193,058 - Pf6N2E2_1020 0.81 +0.4 1,193,058 - Pf6N2E2_1020 0.81 -1.9 1,193,058 - Pf6N2E2_1020 0.81 +1.0 1,193,113 + Pf6N2E2_1020 0.83 -0.5 1,193,130 - Pf6N2E2_1020 0.84 +0.7 1,193,144 + Pf6N2E2_1020 0.85 +0.3 1,193,145 - Pf6N2E2_1020 0.85 +0.9 1,193,392 - +1.3 1,193,628 + +0.5 1,193,734 + -0.2 1,193,883 + -2.1 1,193,895 - -1.3 1,193,895 - -0.6 1,193,998 + Pf6N2E2_1021 0.13 +0.2 1,193,999 - Pf6N2E2_1021 0.13 -0.1 1,193,999 - Pf6N2E2_1021 0.13 -1.8 1,194,030 + Pf6N2E2_1021 0.16 -1.9 1,194,030 + Pf6N2E2_1021 0.16 -0.4 1,194,030 + Pf6N2E2_1021 0.16 -0.2 1,194,031 - Pf6N2E2_1021 0.16 -0.5 1,194,031 - Pf6N2E2_1021 0.16 -0.7 1,194,409 + Pf6N2E2_1021 0.56 +0.8 1,194,409 + Pf6N2E2_1021 0.56 -0.2 1,194,409 + Pf6N2E2_1021 0.56 -0.2 1,194,410 - Pf6N2E2_1021 0.56 -1.4 1,194,410 - Pf6N2E2_1021 0.56 -1.3 1,194,410 - Pf6N2E2_1021 0.56 -2.0 1,194,459 - Pf6N2E2_1021 0.61 +0.5 1,194,583 - Pf6N2E2_1021 0.74 +0.7 1,194,628 - Pf6N2E2_1021 0.79 -1.5 1,195,244 + Pf6N2E2_1022 0.34 -0.1 1,195,366 + Pf6N2E2_1022 0.45 +0.7 1,195,396 + Pf6N2E2_1022 0.47 -0.1 1,195,733 + Pf6N2E2_1022 0.76 +1.0
Or see this region's nucleotide sequence