Experiment: PYE with Paraquat dichloride 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01560 and CCNA_01561 are separated by 154 nucleotides CCNA_01561 and CCNA_01562 overlap by 7 nucleotides CCNA_01562 and CCNA_01563 are separated by 6 nucleotides
CCNA_01560: CCNA_01560 - phosphoenolpyruvate carboxylase, at 1,676,914 to 1,679,643
_01560
CCNA_01561: CCNA_01561 - hypothetical protein, at 1,679,798 to 1,680,181
_01561
CCNA_01562: CCNA_01562 - 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase, at 1,680,175 to 1,680,849
_01562
CCNA_01563: CCNA_01563 - 2-dehydro-3-deoxygluconokinase, at 1,680,856 to 1,681,962
_01563
Position (kb)
1679
1680
1681 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1678.817 kb on - strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.819 kb on + strand, within CCNA_01560 at 1678.901 kb on - strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.921 kb on + strand, within CCNA_01560 at 1678.974 kb on - strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.989 kb on + strand, within CCNA_01560 at 1678.993 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1678.998 kb on - strand, within CCNA_01560 at 1679.001 kb on + strand, within CCNA_01560 at 1679.009 kb on + strand, within CCNA_01560 at 1679.017 kb on - strand, within CCNA_01560 at 1679.041 kb on + strand, within CCNA_01560 at 1679.127 kb on + strand, within CCNA_01560 at 1679.127 kb on + strand, within CCNA_01560 at 1679.142 kb on + strand, within CCNA_01560 at 1679.153 kb on + strand, within CCNA_01560 at 1679.153 kb on + strand, within CCNA_01560 at 1679.161 kb on + strand, within CCNA_01560 at 1679.161 kb on - strand, within CCNA_01560 at 1679.161 kb on - strand, within CCNA_01560 at 1679.202 kb on + strand, within CCNA_01560 at 1679.205 kb on - strand, within CCNA_01560 at 1679.288 kb on + strand, within CCNA_01560 at 1679.310 kb on + strand, within CCNA_01560 at 1679.314 kb on - strand, within CCNA_01560 at 1679.317 kb on + strand, within CCNA_01560 at 1679.370 kb on + strand, within CCNA_01560 at 1679.370 kb on + strand, within CCNA_01560 at 1679.378 kb on - strand at 1679.378 kb on - strand at 1679.378 kb on - strand at 1679.397 kb on + strand at 1679.409 kb on + strand at 1679.426 kb on + strand at 1679.426 kb on + strand at 1679.434 kb on - strand at 1679.460 kb on + strand at 1679.469 kb on - strand at 1679.494 kb on + strand at 1679.495 kb on - strand at 1679.513 kb on - strand at 1679.520 kb on + strand at 1679.858 kb on - strand, within CCNA_01561 at 1679.909 kb on - strand, within CCNA_01561 at 1679.955 kb on - strand, within CCNA_01561 at 1679.955 kb on - strand, within CCNA_01561 at 1679.977 kb on - strand, within CCNA_01561 at 1679.977 kb on - strand, within CCNA_01561 at 1680.025 kb on + strand, within CCNA_01561 at 1680.059 kb on - strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.075 kb on + strand, within CCNA_01561 at 1680.082 kb on + strand, within CCNA_01561 at 1680.178 kb on + strand at 1680.492 kb on + strand, within CCNA_01562 at 1680.533 kb on + strand, within CCNA_01562 at 1680.557 kb on + strand, within CCNA_01562 at 1680.565 kb on - strand, within CCNA_01562 at 1680.588 kb on + strand, within CCNA_01562 at 1680.588 kb on + strand, within CCNA_01562 at 1680.645 kb on + strand, within CCNA_01562 at 1680.674 kb on + strand, within CCNA_01562 at 1680.697 kb on + strand, within CCNA_01562 at 1680.705 kb on - strand, within CCNA_01562 at 1680.746 kb on + strand, within CCNA_01562 at 1680.748 kb on - strand, within CCNA_01562 at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.812 kb on + strand at 1680.820 kb on - strand at 1680.911 kb on - strand at 1680.975 kb on + strand, within CCNA_01563 at 1680.975 kb on + strand, within CCNA_01563 at 1680.981 kb on + strand, within CCNA_01563 at 1681.000 kb on + strand, within CCNA_01563 at 1681.009 kb on - strand, within CCNA_01563 at 1681.021 kb on + strand, within CCNA_01563 at 1681.029 kb on + strand, within CCNA_01563 at 1681.029 kb on + strand, within CCNA_01563 at 1681.066 kb on - strand, within CCNA_01563 at 1681.075 kb on + strand, within CCNA_01563 at 1681.099 kb on + strand, within CCNA_01563 at 1681.125 kb on - strand, within CCNA_01563 at 1681.125 kb on - strand, within CCNA_01563
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Paraquat dichloride 0.03 mg/ml remove 1,678,817 - CCNA_01560 0.70 +1.0 1,678,819 + CCNA_01560 0.70 +0.6 1,678,819 + CCNA_01560 0.70 -0.4 1,678,819 + CCNA_01560 0.70 -1.6 1,678,819 + CCNA_01560 0.70 +0.8 1,678,901 - CCNA_01560 0.73 +1.2 1,678,921 + CCNA_01560 0.74 -0.8 1,678,921 + CCNA_01560 0.74 -1.9 1,678,921 + CCNA_01560 0.74 -0.5 1,678,974 - CCNA_01560 0.75 -0.1 1,678,989 + CCNA_01560 0.76 -0.6 1,678,989 + CCNA_01560 0.76 -2.0 1,678,989 + CCNA_01560 0.76 +0.2 1,678,993 - CCNA_01560 0.76 -0.6 1,678,998 - CCNA_01560 0.76 -0.9 1,678,998 - CCNA_01560 0.76 +1.5 1,678,998 - CCNA_01560 0.76 -1.5 1,679,001 + CCNA_01560 0.76 -1.1 1,679,009 + CCNA_01560 0.77 -0.3 1,679,017 - CCNA_01560 0.77 -0.7 1,679,041 + CCNA_01560 0.78 -0.5 1,679,127 + CCNA_01560 0.81 -1.4 1,679,127 + CCNA_01560 0.81 +0.3 1,679,142 + CCNA_01560 0.82 -0.4 1,679,153 + CCNA_01560 0.82 -0.7 1,679,153 + CCNA_01560 0.82 +1.7 1,679,161 + CCNA_01560 0.82 -0.1 1,679,161 - CCNA_01560 0.82 +0.6 1,679,161 - CCNA_01560 0.82 -0.7 1,679,202 + CCNA_01560 0.84 -0.4 1,679,205 - CCNA_01560 0.84 +0.6 1,679,288 + CCNA_01560 0.87 -1.1 1,679,310 + CCNA_01560 0.88 +1.0 1,679,314 - CCNA_01560 0.88 -1.1 1,679,317 + CCNA_01560 0.88 +1.0 1,679,370 + CCNA_01560 0.90 +0.1 1,679,370 + CCNA_01560 0.90 -1.1 1,679,378 - -0.0 1,679,378 - -0.6 1,679,378 - +0.4 1,679,397 + +0.3 1,679,409 + +0.8 1,679,426 + -0.1 1,679,426 + -0.6 1,679,434 - -0.8 1,679,460 + +1.1 1,679,469 - -0.8 1,679,494 + -0.6 1,679,495 - +1.0 1,679,513 - -1.1 1,679,520 + -0.6 1,679,858 - CCNA_01561 0.16 +0.2 1,679,909 - CCNA_01561 0.29 -0.9 1,679,955 - CCNA_01561 0.41 -1.1 1,679,955 - CCNA_01561 0.41 -2.1 1,679,977 - CCNA_01561 0.47 -0.6 1,679,977 - CCNA_01561 0.47 -0.0 1,680,025 + CCNA_01561 0.59 -0.4 1,680,059 - CCNA_01561 0.68 -1.2 1,680,075 + CCNA_01561 0.72 -0.2 1,680,075 + CCNA_01561 0.72 -1.0 1,680,075 + CCNA_01561 0.72 +0.5 1,680,082 + CCNA_01561 0.74 -0.2 1,680,178 + +0.1 1,680,492 + CCNA_01562 0.47 -1.3 1,680,533 + CCNA_01562 0.53 +0.4 1,680,557 + CCNA_01562 0.57 -0.1 1,680,565 - CCNA_01562 0.58 +0.6 1,680,588 + CCNA_01562 0.61 +1.4 1,680,588 + CCNA_01562 0.61 -0.2 1,680,645 + CCNA_01562 0.70 +1.2 1,680,674 + CCNA_01562 0.74 +0.9 1,680,697 + CCNA_01562 0.77 +0.2 1,680,705 - CCNA_01562 0.79 +0.9 1,680,746 + CCNA_01562 0.85 -0.6 1,680,748 - CCNA_01562 0.85 +0.4 1,680,812 + +0.8 1,680,812 + +1.5 1,680,812 + +1.5 1,680,812 + +0.3 1,680,820 - +1.1 1,680,911 - -0.1 1,680,975 + CCNA_01563 0.11 +0.0 1,680,975 + CCNA_01563 0.11 +0.6 1,680,981 + CCNA_01563 0.11 +0.3 1,681,000 + CCNA_01563 0.13 +0.2 1,681,009 - CCNA_01563 0.14 -0.8 1,681,021 + CCNA_01563 0.15 +1.2 1,681,029 + CCNA_01563 0.16 -1.1 1,681,029 + CCNA_01563 0.16 +0.2 1,681,066 - CCNA_01563 0.19 -1.1 1,681,075 + CCNA_01563 0.20 +0.9 1,681,099 + CCNA_01563 0.22 -0.6 1,681,125 - CCNA_01563 0.24 +3.0 1,681,125 - CCNA_01563 0.24 -0.4
Or see this region's nucleotide sequence