Experiment: Chloramphenicol 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2416 and Shew_2417 are separated by 131 nucleotides Shew_2417 and Shew_2418 are separated by 79 nucleotides Shew_2418 and Shew_2419 are separated by 173 nucleotides
Shew_2416: Shew_2416 - chorismate synthase (RefSeq), at 2,836,447 to 2,837,541
_2416
Shew_2417: Shew_2417 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (RefSeq), at 2,837,673 to 2,838,617
_2417
Shew_2418: Shew_2418 - hypothetical protein (RefSeq), at 2,838,697 to 2,839,224
_2418
Shew_2419: Shew_2419 - phosphohistidine phosphatase, SixA (RefSeq), at 2,839,398 to 2,839,868
_2419
Position (kb)
2837
2838
2839 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2837.093 kb on - strand, within Shew_2416 at 2837.372 kb on + strand, within Shew_2416 at 2837.478 kb on - strand at 2838.019 kb on + strand, within Shew_2417 at 2838.468 kb on + strand, within Shew_2417 at 2838.476 kb on - strand, within Shew_2417 at 2838.839 kb on - strand, within Shew_2418 at 2839.042 kb on - strand, within Shew_2418 at 2839.042 kb on - strand, within Shew_2418 at 2839.131 kb on + strand, within Shew_2418 at 2839.258 kb on + strand at 2839.528 kb on - strand, within Shew_2419 at 2839.567 kb on + strand, within Shew_2419
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 5 mg/ml remove 2,837,093 - Shew_2416 0.59 +1.0 2,837,372 + Shew_2416 0.84 +3.1 2,837,478 - -0.3 2,838,019 + Shew_2417 0.37 -3.4 2,838,468 + Shew_2417 0.84 -2.1 2,838,476 - Shew_2417 0.85 -3.3 2,838,839 - Shew_2418 0.27 +0.8 2,839,042 - Shew_2418 0.65 +0.0 2,839,042 - Shew_2418 0.65 +0.3 2,839,131 + Shew_2418 0.82 -1.6 2,839,258 + +0.2 2,839,528 - Shew_2419 0.28 -0.7 2,839,567 + Shew_2419 0.36 -0.7
Or see this region's nucleotide sequence