Experiment: Chloramphenicol 5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1593 and Shew_1594 are separated by 569 nucleotides Shew_1594 and Shew_1595 overlap by 4 nucleotides Shew_1595 and Shew_1596 overlap by 20 nucleotides
Shew_1593: Shew_1593 - ribonuclease (RefSeq), at 1,823,406 to 1,826,792
_1593
Shew_1594: Shew_1594 - RluA family pseudouridine synthase (RefSeq), at 1,827,362 to 1,828,351
_1594
Shew_1595: Shew_1595 - HAD family hydrolase (RefSeq), at 1,828,348 to 1,829,007
_1595
Shew_1596: Shew_1596 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 1,828,988 to 1,830,361
_1596
Position (kb)
1827
1828
1829 Strain fitness (log2 ratio)
-1
0
1 at 1827.352 kb on + strand at 1827.427 kb on + strand at 1827.442 kb on + strand at 1827.450 kb on - strand at 1827.713 kb on - strand, within Shew_1594 at 1828.044 kb on - strand, within Shew_1594 at 1828.102 kb on - strand, within Shew_1594 at 1828.306 kb on + strand at 1828.551 kb on + strand, within Shew_1595 at 1828.701 kb on - strand, within Shew_1595 at 1828.775 kb on - strand, within Shew_1595 at 1828.900 kb on - strand, within Shew_1595 at 1828.910 kb on - strand, within Shew_1595 at 1828.938 kb on + strand, within Shew_1595 at 1829.250 kb on + strand, within Shew_1596 at 1829.291 kb on + strand, within Shew_1596
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 5 mg/ml remove 1,827,352 + -0.9 1,827,427 + -1.4 1,827,442 + -1.7 1,827,450 - -0.5 1,827,713 - Shew_1594 0.35 -0.8 1,828,044 - Shew_1594 0.69 -0.9 1,828,102 - Shew_1594 0.75 -1.5 1,828,306 + -1.4 1,828,551 + Shew_1595 0.31 -0.4 1,828,701 - Shew_1595 0.53 +0.3 1,828,775 - Shew_1595 0.65 -0.8 1,828,900 - Shew_1595 0.84 +0.2 1,828,910 - Shew_1595 0.85 -0.3 1,828,938 + Shew_1595 0.89 +0.2 1,829,250 + Shew_1596 0.19 +0.2 1,829,291 + Shew_1596 0.22 -0.0
Or see this region's nucleotide sequence