Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_686 and Pf6N2E2_687 are separated by 253 nucleotides Pf6N2E2_687 and Pf6N2E2_688 are separated by 126 nucleotides
Pf6N2E2_686 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 792,843 to 793,577
_686
Pf6N2E2_687 - 4-oxalocrotonate tautomerase (EC 5.3.2.-), at 793,831 to 794,037
_687
Pf6N2E2_688 - Transcriptional regulator, LysR family, at 794,164 to 795,087
_688
Position (kb)
793
794
795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 792.844 kb on + strand at 792.916 kb on + strand at 792.916 kb on + strand at 792.917 kb on - strand, within Pf6N2E2_686 at 792.917 kb on - strand, within Pf6N2E2_686 at 793.229 kb on - strand, within Pf6N2E2_686 at 793.257 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.492 kb on + strand, within Pf6N2E2_686 at 793.679 kb on + strand at 793.691 kb on + strand at 793.691 kb on + strand at 793.691 kb on + strand at 793.833 kb on - strand at 793.888 kb on - strand, within Pf6N2E2_687 at 793.888 kb on - strand, within Pf6N2E2_687 at 793.888 kb on - strand, within Pf6N2E2_687 at 794.013 kb on + strand, within Pf6N2E2_687 at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.098 kb on - strand at 794.098 kb on - strand at 794.098 kb on - strand at 794.101 kb on + strand at 794.101 kb on + strand at 794.102 kb on - strand at 794.102 kb on - strand at 794.156 kb on - strand at 794.156 kb on - strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.202 kb on - strand at 794.202 kb on - strand at 794.202 kb on - strand at 794.374 kb on + strand, within Pf6N2E2_688 at 794.374 kb on + strand, within Pf6N2E2_688 at 794.375 kb on - strand, within Pf6N2E2_688 at 794.375 kb on - strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.692 kb on + strand, within Pf6N2E2_688 at 794.693 kb on - strand, within Pf6N2E2_688 at 794.822 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.840 kb on - strand at 794.840 kb on - strand, within Pf6N2E2_688 at 794.840 kb on - strand, within Pf6N2E2_688 at 794.840 kb on - strand, within Pf6N2E2_688 at 794.908 kb on + strand, within Pf6N2E2_688 at 794.909 kb on - strand, within Pf6N2E2_688 at 794.951 kb on + strand, within Pf6N2E2_688 at 794.952 kb on - strand, within Pf6N2E2_688 at 795.001 kb on + strand at 795.017 kb on - strand at 795.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 792,844 + +0.4 792,916 + +0.7 792,916 + +0.3 792,917 - Pf6N2E2_686 0.10 +0.5 792,917 - Pf6N2E2_686 0.10 -2.4 793,229 - Pf6N2E2_686 0.53 +1.9 793,257 + Pf6N2E2_686 0.56 -1.9 793,490 + Pf6N2E2_686 0.88 -0.4 793,490 + Pf6N2E2_686 0.88 -0.1 793,490 + Pf6N2E2_686 0.88 -2.5 793,490 + Pf6N2E2_686 0.88 -0.2 793,491 - Pf6N2E2_686 0.88 -0.2 793,491 - Pf6N2E2_686 0.88 +0.4 793,491 - Pf6N2E2_686 0.88 +1.9 793,491 - Pf6N2E2_686 0.88 +1.4 793,491 - Pf6N2E2_686 0.88 -0.8 793,491 - Pf6N2E2_686 0.88 -0.0 793,491 - Pf6N2E2_686 0.88 -1.6 793,492 + Pf6N2E2_686 0.88 +0.6 793,679 + -0.0 793,691 + +0.1 793,691 + +0.8 793,691 + -0.7 793,833 - +0.2 793,888 - Pf6N2E2_687 0.28 -0.9 793,888 - Pf6N2E2_687 0.28 -0.4 793,888 - Pf6N2E2_687 0.28 -0.0 794,013 + Pf6N2E2_687 0.88 -1.5 794,097 + -0.4 794,097 + +1.3 794,097 + +1.5 794,097 + +0.3 794,097 + +1.2 794,097 + +0.9 794,097 + -1.3 794,098 - +1.3 794,098 - +0.2 794,098 - -0.7 794,101 + +0.7 794,101 + +0.4 794,102 - +0.6 794,102 - +0.4 794,156 - +0.6 794,156 - +0.9 794,201 + +0.4 794,201 + -0.6 794,201 + -2.2 794,201 + +1.0 794,201 + -0.6 794,201 + -2.6 794,202 - +0.5 794,202 - +0.6 794,202 - +0.6 794,374 + Pf6N2E2_688 0.23 -1.4 794,374 + Pf6N2E2_688 0.23 -1.7 794,375 - Pf6N2E2_688 0.23 +0.4 794,375 - Pf6N2E2_688 0.23 +2.0 794,485 + Pf6N2E2_688 0.35 +1.8 794,485 + Pf6N2E2_688 0.35 +0.1 794,485 + Pf6N2E2_688 0.35 +0.3 794,485 + Pf6N2E2_688 0.35 -2.5 794,486 - Pf6N2E2_688 0.35 -0.3 794,486 - Pf6N2E2_688 0.35 +1.0 794,486 - Pf6N2E2_688 0.35 -0.8 794,486 - Pf6N2E2_688 0.35 +1.8 794,692 + Pf6N2E2_688 0.57 +1.0 794,693 - Pf6N2E2_688 0.57 -0.5 794,822 + Pf6N2E2_688 0.71 +0.5 794,839 + Pf6N2E2_688 0.73 -1.0 794,839 + Pf6N2E2_688 0.73 -0.6 794,839 + Pf6N2E2_688 0.73 -0.3 794,839 + Pf6N2E2_688 0.73 +1.4 794,839 + Pf6N2E2_688 0.73 +1.3 794,840 - +1.9 794,840 - Pf6N2E2_688 0.73 -1.0 794,840 - Pf6N2E2_688 0.73 -2.0 794,840 - Pf6N2E2_688 0.73 -1.1 794,908 + Pf6N2E2_688 0.81 -0.2 794,909 - Pf6N2E2_688 0.81 +0.6 794,951 + Pf6N2E2_688 0.85 +0.6 794,952 - Pf6N2E2_688 0.85 -0.3 795,001 + +0.5 795,017 - -0.9 795,017 - +1.1
Or see this region's nucleotide sequence