Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_6100

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotidesPf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotidesPf6N2E2_6100 and Pf6N2E2_6101 are separated by 61 nucleotidesPf6N2E2_6101 and Pf6N2E2_6102 are separated by 93 nucleotidesPf6N2E2_6102 and Pf6N2E2_79 are separated by 432 nucleotides Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762 _77 Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532 _78 Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773 _6100 Pf6N2E2_6101 - tRNA-Asp-GTC, at 121,835 to 121,908 _6101 Pf6N2E2_6102 - tRNA-Asp-GTC, at 122,002 to 122,075 _6102 Pf6N2E2_79 - Mobile element protein, at 122,508 to 123,776 _79 Position (kb) 121 122Strain fitness (log2 ratio) -2 -1 0 1 2at 121.092 kb on + strand, within Pf6N2E2_78at 121.159 kb on - strand, within Pf6N2E2_78at 121.236 kb on + strand, within Pf6N2E2_78at 121.236 kb on + strand, within Pf6N2E2_78at 121.237 kb on - strand, within Pf6N2E2_78at 121.237 kb on - strand, within Pf6N2E2_78at 121.412 kb on - strand, within Pf6N2E2_78at 121.421 kb on - strand, within Pf6N2E2_78at 121.524 kb on + strandat 121.524 kb on + strandat 121.525 kb on - strandat 121.525 kb on - strandat 121.525 kb on - strandat 121.572 kb on + strandat 121.572 kb on + strandat 121.573 kb on - strandat 121.598 kb on + strandat 121.599 kb on - strandat 121.658 kb on - strandat 121.734 kb on + strand, within Pf6N2E2_6100at 121.734 kb on + strand, within Pf6N2E2_6100at 121.782 kb on + strandat 122.139 kb on + strandat 122.203 kb on + strandat 122.203 kb on + strandat 122.252 kb on - strandat 122.252 kb on - strandat 122.252 kb on - strandat 122.252 kb on - strandat 122.252 kb on - strandat 122.256 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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121,092 + Pf6N2E2_78 0.43 +0.5
121,159 - Pf6N2E2_78 0.52 +0.6
121,236 + Pf6N2E2_78 0.62 -1.5
121,236 + Pf6N2E2_78 0.62 +0.1
121,237 - Pf6N2E2_78 0.62 -1.1
121,237 - Pf6N2E2_78 0.62 +0.2
121,412 - Pf6N2E2_78 0.84 +0.3
121,421 - Pf6N2E2_78 0.86 -0.3
121,524 + -0.0
121,524 + +0.6
121,525 - +0.8
121,525 - +0.2
121,525 - +0.9
121,572 + -1.2
121,572 + +1.0
121,573 - +1.1
121,598 + -0.1
121,599 - -0.0
121,658 - -2.4
121,734 + Pf6N2E2_6100 0.45 -1.6
121,734 + Pf6N2E2_6100 0.45 -2.7
121,782 + +1.3
122,139 + -0.7
122,203 + +0.5
122,203 + +1.9
122,252 - +1.3
122,252 - +0.1
122,252 - +1.2
122,252 - -0.1
122,252 - +0.3
122,256 - +1.1

Or see this region's nucleotide sequence