Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5892 and Pf6N2E2_5893 are separated by 196 nucleotides Pf6N2E2_5893 and Pf6N2E2_6166 are separated by 371 nucleotides Pf6N2E2_6166 and Pf6N2E2_6167 are separated by 5 nucleotides Pf6N2E2_6167 and Pf6N2E2_6168 are separated by 108 nucleotides Pf6N2E2_6168 and Pf6N2E2_6169 are separated by 6 nucleotides
Pf6N2E2_5892 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 6,710,613 to 6,712,580
_5892
Pf6N2E2_5893 - FIG00956448: hypothetical protein, at 6,712,777 to 6,713,535
_5893
Pf6N2E2_6166 - tRNA-Glu-TTC, at 6,713,907 to 6,713,979
_6166
Pf6N2E2_6167 - tRNA-Gly-GCC, at 6,713,985 to 6,714,057
_6167
Pf6N2E2_6168 - tRNA-Glu-TTC, at 6,714,166 to 6,714,238
_6168
Pf6N2E2_6169 - tRNA-Gly-GCC, at 6,714,245 to 6,714,317
_6169
Position (kb)
6712
6713
6714 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6711.983 kb on + strand, within Pf6N2E2_5892 at 6711.983 kb on + strand, within Pf6N2E2_5892 at 6711.983 kb on + strand, within Pf6N2E2_5892 at 6711.983 kb on + strand, within Pf6N2E2_5892 at 6711.984 kb on - strand, within Pf6N2E2_5892 at 6712.333 kb on + strand, within Pf6N2E2_5892 at 6712.333 kb on + strand, within Pf6N2E2_5892 at 6712.333 kb on + strand, within Pf6N2E2_5892 at 6712.359 kb on - strand, within Pf6N2E2_5892 at 6712.631 kb on + strand at 6712.631 kb on + strand at 6712.632 kb on - strand at 6712.632 kb on - strand at 6712.639 kb on + strand at 6712.639 kb on + strand at 6712.645 kb on - strand at 6712.776 kb on - strand at 6712.785 kb on - strand at 6712.920 kb on - strand, within Pf6N2E2_5893 at 6712.937 kb on + strand, within Pf6N2E2_5893 at 6712.937 kb on + strand, within Pf6N2E2_5893 at 6712.937 kb on + strand, within Pf6N2E2_5893 at 6712.938 kb on - strand, within Pf6N2E2_5893 at 6712.938 kb on - strand, within Pf6N2E2_5893 at 6712.967 kb on + strand, within Pf6N2E2_5893 at 6712.967 kb on + strand, within Pf6N2E2_5893 at 6712.968 kb on - strand, within Pf6N2E2_5893 at 6712.968 kb on - strand, within Pf6N2E2_5893 at 6713.415 kb on + strand, within Pf6N2E2_5893 at 6713.416 kb on - strand, within Pf6N2E2_5893 at 6713.431 kb on + strand, within Pf6N2E2_5893 at 6713.432 kb on - strand, within Pf6N2E2_5893 at 6713.432 kb on - strand, within Pf6N2E2_5893 at 6713.432 kb on - strand, within Pf6N2E2_5893 at 6713.432 kb on - strand, within Pf6N2E2_5893 at 6713.432 kb on - strand, within Pf6N2E2_5893 at 6713.483 kb on + strand at 6713.483 kb on + strand at 6713.484 kb on - strand at 6713.484 kb on - strand at 6713.562 kb on + strand at 6713.588 kb on - strand at 6713.610 kb on + strand at 6713.610 kb on + strand at 6713.611 kb on - strand at 6713.630 kb on - strand at 6713.649 kb on + strand at 6713.651 kb on + strand at 6713.651 kb on + strand at 6713.651 kb on + strand at 6713.651 kb on + strand at 6713.652 kb on - strand at 6713.702 kb on + strand at 6713.702 kb on + strand at 6713.703 kb on - strand at 6713.704 kb on + strand at 6713.704 kb on + strand at 6713.704 kb on + strand at 6713.705 kb on - strand at 6713.727 kb on + strand at 6713.727 kb on + strand at 6713.727 kb on + strand at 6713.727 kb on + strand at 6713.728 kb on - strand at 6713.728 kb on - strand at 6713.729 kb on + strand at 6713.900 kb on + strand at 6713.900 kb on + strand at 6714.140 kb on + strand at 6714.140 kb on + strand at 6714.394 kb on + strand at 6714.465 kb on + strand at 6714.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,711,983 + Pf6N2E2_5892 0.70 -0.0 6,711,983 + Pf6N2E2_5892 0.70 -0.4 6,711,983 + Pf6N2E2_5892 0.70 -1.2 6,711,983 + Pf6N2E2_5892 0.70 -0.3 6,711,984 - Pf6N2E2_5892 0.70 -1.8 6,712,333 + Pf6N2E2_5892 0.87 -0.6 6,712,333 + Pf6N2E2_5892 0.87 -0.2 6,712,333 + Pf6N2E2_5892 0.87 -0.1 6,712,359 - Pf6N2E2_5892 0.89 +0.4 6,712,631 + +1.5 6,712,631 + -0.3 6,712,632 - +0.6 6,712,632 - -0.8 6,712,639 + +0.0 6,712,639 + -0.2 6,712,645 - +0.8 6,712,776 - -1.2 6,712,785 - -2.5 6,712,920 - Pf6N2E2_5893 0.19 -2.3 6,712,937 + Pf6N2E2_5893 0.21 -2.1 6,712,937 + Pf6N2E2_5893 0.21 -2.0 6,712,937 + Pf6N2E2_5893 0.21 -1.7 6,712,938 - Pf6N2E2_5893 0.21 -2.5 6,712,938 - Pf6N2E2_5893 0.21 -2.6 6,712,967 + Pf6N2E2_5893 0.25 -3.2 6,712,967 + Pf6N2E2_5893 0.25 -0.8 6,712,968 - Pf6N2E2_5893 0.25 -2.5 6,712,968 - Pf6N2E2_5893 0.25 -2.8 6,713,415 + Pf6N2E2_5893 0.84 -0.0 6,713,416 - Pf6N2E2_5893 0.84 -2.4 6,713,431 + Pf6N2E2_5893 0.86 -1.8 6,713,432 - Pf6N2E2_5893 0.86 -1.7 6,713,432 - Pf6N2E2_5893 0.86 -1.2 6,713,432 - Pf6N2E2_5893 0.86 -2.9 6,713,432 - Pf6N2E2_5893 0.86 -1.7 6,713,432 - Pf6N2E2_5893 0.86 -1.5 6,713,483 + +0.0 6,713,483 + -1.9 6,713,484 - +0.6 6,713,484 - -2.4 6,713,562 + -1.7 6,713,588 - -0.0 6,713,610 + -1.2 6,713,610 + -1.8 6,713,611 - -0.7 6,713,630 - -1.6 6,713,649 + -0.7 6,713,651 + +0.6 6,713,651 + -1.5 6,713,651 + +0.5 6,713,651 + +0.3 6,713,652 - +0.2 6,713,702 + +0.2 6,713,702 + -3.3 6,713,703 - +0.4 6,713,704 + -2.3 6,713,704 + -0.3 6,713,704 + -1.7 6,713,705 - +1.6 6,713,727 + +1.8 6,713,727 + -1.0 6,713,727 + -0.0 6,713,727 + -0.3 6,713,728 - -2.1 6,713,728 - +0.4 6,713,729 + +1.7 6,713,900 + -1.5 6,713,900 + -3.1 6,714,140 + -1.2 6,714,140 + -1.1 6,714,394 + -0.5 6,714,465 + +0.5 6,714,469 - -2.3
Or see this region's nucleotide sequence