Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5875 and Pf6N2E2_5876 are separated by 30 nucleotides Pf6N2E2_5876 and Pf6N2E2_5877 overlap by 4 nucleotides Pf6N2E2_5877 and Pf6N2E2_5878 are separated by 52 nucleotides Pf6N2E2_5878 and Pf6N2E2_5879 are separated by 296 nucleotides
Pf6N2E2_5875 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 6,696,633 to 6,697,664
_5875
Pf6N2E2_5876 - Lipase (EC 3.1.1.3), at 6,697,695 to 6,698,042
_5876
Pf6N2E2_5877 - Phosphohistidine phosphatase SixA, at 6,698,039 to 6,698,491
_5877
Pf6N2E2_5878 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 6,698,544 to 6,698,987
_5878
Pf6N2E2_5879 - FIG00955363: hypothetical protein, at 6,699,284 to 6,700,078
_5879
Position (kb)
6698
6699 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6697.836 kb on + strand, within Pf6N2E2_5876 at 6697.836 kb on + strand, within Pf6N2E2_5876 at 6697.900 kb on + strand, within Pf6N2E2_5876 at 6697.901 kb on - strand, within Pf6N2E2_5876 at 6697.901 kb on - strand, within Pf6N2E2_5876 at 6697.901 kb on - strand, within Pf6N2E2_5876 at 6697.901 kb on - strand, within Pf6N2E2_5876 at 6698.038 kb on + strand at 6698.038 kb on + strand at 6698.039 kb on - strand at 6698.181 kb on - strand, within Pf6N2E2_5877 at 6698.181 kb on - strand, within Pf6N2E2_5877 at 6698.181 kb on - strand, within Pf6N2E2_5877 at 6698.181 kb on - strand, within Pf6N2E2_5877 at 6698.181 kb on - strand, within Pf6N2E2_5877 at 6698.600 kb on + strand, within Pf6N2E2_5878 at 6698.600 kb on + strand, within Pf6N2E2_5878 at 6698.601 kb on - strand, within Pf6N2E2_5878 at 6698.757 kb on + strand, within Pf6N2E2_5878 at 6698.757 kb on + strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.758 kb on - strand, within Pf6N2E2_5878 at 6698.784 kb on + strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.785 kb on - strand, within Pf6N2E2_5878 at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.996 kb on + strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6698.997 kb on - strand at 6699.069 kb on - strand at 6699.069 kb on - strand at 6699.173 kb on + strand at 6699.173 kb on + strand at 6699.217 kb on + strand at 6699.217 kb on + strand at 6699.335 kb on + strand at 6699.369 kb on - strand, within Pf6N2E2_5879 at 6699.551 kb on + strand, within Pf6N2E2_5879 at 6699.765 kb on - strand, within Pf6N2E2_5879 at 6699.840 kb on - strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.958 kb on + strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879 at 6699.959 kb on - strand, within Pf6N2E2_5879
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,697,836 + Pf6N2E2_5876 0.41 -0.9 6,697,836 + Pf6N2E2_5876 0.41 -3.0 6,697,900 + Pf6N2E2_5876 0.59 -2.7 6,697,901 - Pf6N2E2_5876 0.59 +1.7 6,697,901 - Pf6N2E2_5876 0.59 -1.4 6,697,901 - Pf6N2E2_5876 0.59 +0.1 6,697,901 - Pf6N2E2_5876 0.59 -0.4 6,698,038 + -1.2 6,698,038 + -0.2 6,698,039 - -0.0 6,698,181 - Pf6N2E2_5877 0.31 -1.5 6,698,181 - Pf6N2E2_5877 0.31 +0.1 6,698,181 - Pf6N2E2_5877 0.31 +1.4 6,698,181 - Pf6N2E2_5877 0.31 +1.5 6,698,181 - Pf6N2E2_5877 0.31 -1.0 6,698,600 + Pf6N2E2_5878 0.13 +1.0 6,698,600 + Pf6N2E2_5878 0.13 +0.4 6,698,601 - Pf6N2E2_5878 0.13 +0.4 6,698,757 + Pf6N2E2_5878 0.48 -1.8 6,698,757 + Pf6N2E2_5878 0.48 -0.6 6,698,758 - Pf6N2E2_5878 0.48 -1.6 6,698,758 - Pf6N2E2_5878 0.48 -1.8 6,698,758 - Pf6N2E2_5878 0.48 +0.3 6,698,758 - Pf6N2E2_5878 0.48 -1.2 6,698,758 - Pf6N2E2_5878 0.48 -0.4 6,698,784 + Pf6N2E2_5878 0.54 -0.7 6,698,785 - Pf6N2E2_5878 0.54 -0.2 6,698,785 - Pf6N2E2_5878 0.54 -0.8 6,698,785 - Pf6N2E2_5878 0.54 -2.2 6,698,785 - Pf6N2E2_5878 0.54 -3.0 6,698,785 - Pf6N2E2_5878 0.54 +0.8 6,698,996 + +0.8 6,698,996 + +1.4 6,698,996 + +0.7 6,698,996 + +0.5 6,698,996 + -0.6 6,698,996 + -0.4 6,698,996 + -2.4 6,698,996 + -0.2 6,698,996 + +0.9 6,698,996 + +1.1 6,698,996 + +0.3 6,698,996 + +1.5 6,698,997 - -0.2 6,698,997 - -1.0 6,698,997 - +1.1 6,698,997 - -0.8 6,698,997 - -1.3 6,699,069 - -0.2 6,699,069 - -1.8 6,699,173 + -0.9 6,699,173 + +0.4 6,699,217 + -0.1 6,699,217 + -0.2 6,699,335 + +0.3 6,699,369 - Pf6N2E2_5879 0.11 -1.1 6,699,551 + Pf6N2E2_5879 0.34 -0.9 6,699,765 - Pf6N2E2_5879 0.61 +0.0 6,699,840 - Pf6N2E2_5879 0.70 +0.2 6,699,958 + Pf6N2E2_5879 0.85 +0.4 6,699,958 + Pf6N2E2_5879 0.85 -0.7 6,699,958 + Pf6N2E2_5879 0.85 +1.1 6,699,958 + Pf6N2E2_5879 0.85 -0.1 6,699,959 - Pf6N2E2_5879 0.85 -0.3 6,699,959 - Pf6N2E2_5879 0.85 -3.2 6,699,959 - Pf6N2E2_5879 0.85 -0.2 6,699,959 - Pf6N2E2_5879 0.85 +1.2 6,699,959 - Pf6N2E2_5879 0.85 +2.1 6,699,959 - Pf6N2E2_5879 0.85 -0.2 6,699,959 - Pf6N2E2_5879 0.85 -1.8 6,699,959 - Pf6N2E2_5879 0.85 -0.8 6,699,959 - Pf6N2E2_5879 0.85 -1.9 6,699,959 - Pf6N2E2_5879 0.85 +0.3 6,699,959 - Pf6N2E2_5879 0.85 +0.5 6,699,959 - Pf6N2E2_5879 0.85 +0.3 6,699,959 - Pf6N2E2_5879 0.85 +0.1 6,699,959 - Pf6N2E2_5879 0.85 -1.4 6,699,959 - Pf6N2E2_5879 0.85 -1.6
Or see this region's nucleotide sequence