Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_584 and Pf6N2E2_585 overlap by 4 nucleotides Pf6N2E2_585 and Pf6N2E2_586 are separated by 267 nucleotides
Pf6N2E2_584 - ABC transporter, periplasmic substrate-binding protein, at 659,542 to 661,395
_584
Pf6N2E2_585 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 661,392 to 663,206
_585
Pf6N2E2_586 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 663,474 to 664,874
_586
Position (kb)
661
662
663
664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 660.421 kb on + strand, within Pf6N2E2_584 at 660.452 kb on + strand, within Pf6N2E2_584 at 660.453 kb on - strand, within Pf6N2E2_584 at 660.536 kb on + strand, within Pf6N2E2_584 at 660.536 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.629 kb on + strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.630 kb on - strand, within Pf6N2E2_584 at 660.644 kb on - strand, within Pf6N2E2_584 at 660.654 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.692 kb on + strand, within Pf6N2E2_584 at 660.693 kb on - strand, within Pf6N2E2_584 at 660.693 kb on - strand, within Pf6N2E2_584 at 660.700 kb on + strand, within Pf6N2E2_584 at 660.861 kb on + strand, within Pf6N2E2_584 at 660.861 kb on + strand, within Pf6N2E2_584 at 660.929 kb on + strand, within Pf6N2E2_584 at 660.933 kb on - strand, within Pf6N2E2_584 at 661.041 kb on - strand, within Pf6N2E2_584 at 661.041 kb on - strand, within Pf6N2E2_584 at 661.097 kb on + strand, within Pf6N2E2_584 at 661.142 kb on + strand, within Pf6N2E2_584 at 661.304 kb on + strand at 661.304 kb on + strand at 661.305 kb on - strand at 661.444 kb on + strand at 661.444 kb on + strand at 661.444 kb on + strand at 661.445 kb on - strand at 661.481 kb on - strand at 661.495 kb on + strand at 661.495 kb on + strand at 661.496 kb on - strand at 661.496 kb on - strand at 661.504 kb on + strand at 661.522 kb on + strand at 661.523 kb on - strand at 661.643 kb on - strand, within Pf6N2E2_585 at 661.643 kb on - strand, within Pf6N2E2_585 at 661.685 kb on - strand, within Pf6N2E2_585 at 661.771 kb on + strand, within Pf6N2E2_585 at 661.772 kb on - strand, within Pf6N2E2_585 at 661.772 kb on - strand, within Pf6N2E2_585 at 661.834 kb on + strand, within Pf6N2E2_585 at 661.834 kb on + strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 661.835 kb on - strand, within Pf6N2E2_585 at 662.122 kb on + strand, within Pf6N2E2_585 at 662.123 kb on - strand, within Pf6N2E2_585 at 662.123 kb on - strand, within Pf6N2E2_585 at 662.140 kb on + strand, within Pf6N2E2_585 at 662.141 kb on - strand, within Pf6N2E2_585 at 662.141 kb on - strand, within Pf6N2E2_585 at 662.205 kb on + strand, within Pf6N2E2_585 at 662.206 kb on - strand, within Pf6N2E2_585 at 662.295 kb on + strand, within Pf6N2E2_585 at 662.295 kb on + strand, within Pf6N2E2_585 at 662.296 kb on - strand, within Pf6N2E2_585 at 662.296 kb on - strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585 at 662.362 kb on + strand, within Pf6N2E2_585 at 662.416 kb on + strand, within Pf6N2E2_585 at 662.416 kb on + strand, within Pf6N2E2_585 at 662.440 kb on + strand, within Pf6N2E2_585 at 662.440 kb on + strand, within Pf6N2E2_585 at 662.440 kb on + strand, within Pf6N2E2_585 at 662.441 kb on - strand, within Pf6N2E2_585 at 662.447 kb on - strand, within Pf6N2E2_585 at 662.706 kb on + strand, within Pf6N2E2_585 at 662.782 kb on + strand, within Pf6N2E2_585 at 662.782 kb on + strand, within Pf6N2E2_585 at 662.887 kb on + strand, within Pf6N2E2_585 at 662.887 kb on + strand, within Pf6N2E2_585 at 662.887 kb on + strand, within Pf6N2E2_585 at 663.016 kb on + strand, within Pf6N2E2_585 at 663.142 kb on + strand at 663.219 kb on + strand at 663.219 kb on + strand at 663.219 kb on + strand at 663.219 kb on + strand at 663.220 kb on - strand at 663.220 kb on - strand at 663.220 kb on - strand at 663.257 kb on + strand at 663.266 kb on + strand at 663.266 kb on + strand at 663.267 kb on - strand at 663.267 kb on - strand at 663.271 kb on + strand at 663.281 kb on + strand at 663.391 kb on + strand at 663.488 kb on - strand at 663.586 kb on + strand at 663.586 kb on + strand at 663.586 kb on + strand at 663.587 kb on - strand at 663.587 kb on - strand at 663.587 kb on - strand at 663.587 kb on - strand at 663.587 kb on - strand at 663.613 kb on + strand at 663.614 kb on - strand, within Pf6N2E2_586 at 663.614 kb on - strand, within Pf6N2E2_586 at 663.614 kb on - strand, within Pf6N2E2_586 at 663.826 kb on + strand, within Pf6N2E2_586 at 664.139 kb on - strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 660,421 + Pf6N2E2_584 0.47 -1.3 660,452 + Pf6N2E2_584 0.49 +0.1 660,453 - Pf6N2E2_584 0.49 -0.6 660,536 + Pf6N2E2_584 0.54 -3.7 660,536 + Pf6N2E2_584 0.54 -1.1 660,629 + Pf6N2E2_584 0.59 -0.7 660,629 + Pf6N2E2_584 0.59 -0.5 660,629 + Pf6N2E2_584 0.59 -1.3 660,630 - Pf6N2E2_584 0.59 -1.5 660,630 - Pf6N2E2_584 0.59 -1.7 660,630 - Pf6N2E2_584 0.59 -0.6 660,630 - Pf6N2E2_584 0.59 -1.9 660,644 - Pf6N2E2_584 0.59 -1.5 660,654 + Pf6N2E2_584 0.60 -3.0 660,692 + Pf6N2E2_584 0.62 -2.3 660,692 + Pf6N2E2_584 0.62 -1.7 660,692 + Pf6N2E2_584 0.62 -1.1 660,692 + Pf6N2E2_584 0.62 -1.0 660,693 - Pf6N2E2_584 0.62 -0.3 660,693 - Pf6N2E2_584 0.62 -1.5 660,700 + Pf6N2E2_584 0.62 -1.0 660,861 + Pf6N2E2_584 0.71 -3.0 660,861 + Pf6N2E2_584 0.71 -0.8 660,929 + Pf6N2E2_584 0.75 -1.2 660,933 - Pf6N2E2_584 0.75 -1.1 661,041 - Pf6N2E2_584 0.81 -1.0 661,041 - Pf6N2E2_584 0.81 -1.0 661,097 + Pf6N2E2_584 0.84 -1.1 661,142 + Pf6N2E2_584 0.86 -1.4 661,304 + -0.9 661,304 + -2.6 661,305 - -2.8 661,444 + -1.5 661,444 + -1.9 661,444 + -2.1 661,445 - -1.8 661,481 - +1.0 661,495 + -1.2 661,495 + -0.8 661,496 - -0.5 661,496 - -0.4 661,504 + -1.2 661,522 + -3.4 661,523 - +0.8 661,643 - Pf6N2E2_585 0.14 -0.8 661,643 - Pf6N2E2_585 0.14 +2.9 661,685 - Pf6N2E2_585 0.16 +0.3 661,771 + Pf6N2E2_585 0.21 -1.3 661,772 - Pf6N2E2_585 0.21 +0.0 661,772 - Pf6N2E2_585 0.21 +1.1 661,834 + Pf6N2E2_585 0.24 -2.9 661,834 + Pf6N2E2_585 0.24 -0.3 661,835 - Pf6N2E2_585 0.24 -0.3 661,835 - Pf6N2E2_585 0.24 +0.7 661,835 - Pf6N2E2_585 0.24 +0.0 661,835 - Pf6N2E2_585 0.24 -0.8 662,122 + Pf6N2E2_585 0.40 +0.2 662,123 - Pf6N2E2_585 0.40 -0.3 662,123 - Pf6N2E2_585 0.40 -0.1 662,140 + Pf6N2E2_585 0.41 -0.6 662,141 - Pf6N2E2_585 0.41 +0.5 662,141 - Pf6N2E2_585 0.41 +0.2 662,205 + Pf6N2E2_585 0.45 -1.7 662,206 - Pf6N2E2_585 0.45 +0.8 662,295 + Pf6N2E2_585 0.50 +0.0 662,295 + Pf6N2E2_585 0.50 -0.7 662,296 - Pf6N2E2_585 0.50 -0.5 662,296 - Pf6N2E2_585 0.50 -1.2 662,362 + Pf6N2E2_585 0.53 -0.6 662,362 + Pf6N2E2_585 0.53 -2.0 662,362 + Pf6N2E2_585 0.53 -1.8 662,416 + Pf6N2E2_585 0.56 -3.0 662,416 + Pf6N2E2_585 0.56 -0.7 662,440 + Pf6N2E2_585 0.58 -0.1 662,440 + Pf6N2E2_585 0.58 +0.9 662,440 + Pf6N2E2_585 0.58 -1.2 662,441 - Pf6N2E2_585 0.58 -0.5 662,447 - Pf6N2E2_585 0.58 -0.5 662,706 + Pf6N2E2_585 0.72 -0.2 662,782 + Pf6N2E2_585 0.77 -2.4 662,782 + Pf6N2E2_585 0.77 -2.0 662,887 + Pf6N2E2_585 0.82 -0.3 662,887 + Pf6N2E2_585 0.82 -0.9 662,887 + Pf6N2E2_585 0.82 -2.0 663,016 + Pf6N2E2_585 0.89 -1.3 663,142 + -1.2 663,219 + -0.5 663,219 + -1.1 663,219 + -1.6 663,219 + -1.4 663,220 - -0.2 663,220 - -0.1 663,220 - -3.2 663,257 + -2.0 663,266 + -0.7 663,266 + -1.9 663,267 - +0.4 663,267 - +0.6 663,271 + -0.0 663,281 + -0.1 663,391 + +0.0 663,488 - -1.0 663,586 + -0.8 663,586 + +0.4 663,586 + -0.2 663,587 - -0.9 663,587 - +0.1 663,587 - +0.2 663,587 - -1.4 663,587 - -0.8 663,613 + -0.3 663,614 - Pf6N2E2_586 0.10 +0.5 663,614 - Pf6N2E2_586 0.10 +0.8 663,614 - Pf6N2E2_586 0.10 +0.2 663,826 + Pf6N2E2_586 0.25 -1.9 664,139 - Pf6N2E2_586 0.47 -2.6 664,189 + Pf6N2E2_586 0.51 -0.3 664,189 + Pf6N2E2_586 0.51 -0.8 664,189 + Pf6N2E2_586 0.51 -3.4 664,190 - Pf6N2E2_586 0.51 -0.5 664,190 - Pf6N2E2_586 0.51 -2.9 664,190 - Pf6N2E2_586 0.51 -2.6 664,190 - Pf6N2E2_586 0.51 -1.3 664,190 - Pf6N2E2_586 0.51 -1.0 664,190 - Pf6N2E2_586 0.51 -0.7 664,190 - Pf6N2E2_586 0.51 -2.6
Or see this region's nucleotide sequence