Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_580 and Pf6N2E2_581 are separated by 19 nucleotides Pf6N2E2_581 and Pf6N2E2_582 are separated by 1 nucleotides
Pf6N2E2_580 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 655,017 to 655,811
_580
Pf6N2E2_581 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 655,831 to 657,441
_581
Pf6N2E2_582 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 657,443 to 658,462
_582
Position (kb)
655
656
657
658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 654.865 kb on + strand at 654.866 kb on - strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.873 kb on + strand at 654.925 kb on + strand at 654.925 kb on + strand at 654.926 kb on - strand at 654.936 kb on - strand at 654.936 kb on - strand at 654.936 kb on - strand at 655.018 kb on + strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.882 kb on - strand at 655.884 kb on - strand at 655.884 kb on - strand at 656.010 kb on - strand, within Pf6N2E2_581 at 656.010 kb on - strand, within Pf6N2E2_581 at 656.010 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.022 kb on - strand, within Pf6N2E2_581 at 656.071 kb on - strand, within Pf6N2E2_581 at 656.210 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.300 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.550 kb on - strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.758 kb on + strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 656.759 kb on - strand, within Pf6N2E2_581 at 657.221 kb on + strand, within Pf6N2E2_581 at 657.221 kb on + strand, within Pf6N2E2_581 at 657.222 kb on - strand, within Pf6N2E2_581 at 657.430 kb on - strand at 657.762 kb on + strand, within Pf6N2E2_582 at 657.763 kb on - strand, within Pf6N2E2_582 at 657.894 kb on + strand, within Pf6N2E2_582 at 657.960 kb on + strand, within Pf6N2E2_582 at 657.960 kb on + strand, within Pf6N2E2_582 at 657.961 kb on - strand, within Pf6N2E2_582 at 658.028 kb on + strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.029 kb on - strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.050 kb on + strand, within Pf6N2E2_582 at 658.051 kb on - strand, within Pf6N2E2_582 at 658.051 kb on - strand, within Pf6N2E2_582 at 658.144 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.159 kb on - strand, within Pf6N2E2_582 at 658.221 kb on + strand, within Pf6N2E2_582 at 658.221 kb on + strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.222 kb on - strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.233 kb on + strand, within Pf6N2E2_582 at 658.234 kb on - strand, within Pf6N2E2_582 at 658.314 kb on + strand, within Pf6N2E2_582 at 658.315 kb on - strand, within Pf6N2E2_582
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 654,865 + -0.6 654,866 - +2.4 654,873 + -1.3 654,873 + -0.3 654,873 + -1.3 654,873 + -1.9 654,873 + +0.9 654,873 + +0.4 654,873 + -1.6 654,925 + +0.5 654,925 + -0.4 654,926 - -0.1 654,936 - +0.0 654,936 - -0.4 654,936 - +0.0 655,018 + -0.5 655,882 - -0.8 655,882 - +0.6 655,882 - +1.2 655,882 - +0.4 655,882 - -0.3 655,882 - -2.7 655,882 - -2.4 655,882 - +1.2 655,882 - -1.5 655,884 - -1.0 655,884 - -0.8 656,010 - Pf6N2E2_581 0.11 +1.1 656,010 - Pf6N2E2_581 0.11 -2.3 656,010 - Pf6N2E2_581 0.11 -1.9 656,022 - Pf6N2E2_581 0.12 -0.6 656,022 - Pf6N2E2_581 0.12 -1.4 656,022 - Pf6N2E2_581 0.12 +0.1 656,071 - Pf6N2E2_581 0.15 +1.1 656,210 - Pf6N2E2_581 0.24 +0.6 656,300 - Pf6N2E2_581 0.29 +1.0 656,300 - Pf6N2E2_581 0.29 -0.7 656,300 - Pf6N2E2_581 0.29 -0.9 656,550 - Pf6N2E2_581 0.45 -0.6 656,550 - Pf6N2E2_581 0.45 -1.3 656,550 - Pf6N2E2_581 0.45 +1.1 656,550 - Pf6N2E2_581 0.45 -0.6 656,550 - Pf6N2E2_581 0.45 +1.4 656,550 - Pf6N2E2_581 0.45 -1.2 656,550 - Pf6N2E2_581 0.45 -0.6 656,758 + Pf6N2E2_581 0.58 -0.2 656,758 + Pf6N2E2_581 0.58 -1.7 656,758 + Pf6N2E2_581 0.58 -1.2 656,758 + Pf6N2E2_581 0.58 -0.8 656,758 + Pf6N2E2_581 0.58 -1.0 656,758 + Pf6N2E2_581 0.58 -0.2 656,758 + Pf6N2E2_581 0.58 -0.5 656,759 - Pf6N2E2_581 0.58 -0.3 656,759 - Pf6N2E2_581 0.58 +0.0 656,759 - Pf6N2E2_581 0.58 +2.3 656,759 - Pf6N2E2_581 0.58 +2.1 657,221 + Pf6N2E2_581 0.86 -0.7 657,221 + Pf6N2E2_581 0.86 +0.2 657,222 - Pf6N2E2_581 0.86 +0.1 657,430 - +1.0 657,762 + Pf6N2E2_582 0.31 -1.2 657,763 - Pf6N2E2_582 0.31 -2.3 657,894 + Pf6N2E2_582 0.44 -1.7 657,960 + Pf6N2E2_582 0.51 +0.9 657,960 + Pf6N2E2_582 0.51 -1.2 657,961 - Pf6N2E2_582 0.51 -3.7 658,028 + Pf6N2E2_582 0.57 -1.8 658,029 - Pf6N2E2_582 0.57 -2.4 658,029 - Pf6N2E2_582 0.57 -2.2 658,029 - Pf6N2E2_582 0.57 -1.5 658,029 - Pf6N2E2_582 0.57 -1.2 658,050 + Pf6N2E2_582 0.60 -2.4 658,050 + Pf6N2E2_582 0.60 -0.7 658,050 + Pf6N2E2_582 0.60 +0.3 658,051 - Pf6N2E2_582 0.60 -1.2 658,051 - Pf6N2E2_582 0.60 +0.4 658,144 - Pf6N2E2_582 0.69 -2.1 658,159 - Pf6N2E2_582 0.70 -1.6 658,159 - Pf6N2E2_582 0.70 -0.6 658,159 - Pf6N2E2_582 0.70 -3.4 658,221 + Pf6N2E2_582 0.76 -1.9 658,221 + Pf6N2E2_582 0.76 -2.0 658,222 - Pf6N2E2_582 0.76 -3.4 658,222 - Pf6N2E2_582 0.76 -1.3 658,222 - Pf6N2E2_582 0.76 -1.3 658,222 - Pf6N2E2_582 0.76 -0.2 658,222 - Pf6N2E2_582 0.76 -1.4 658,233 + Pf6N2E2_582 0.77 +1.9 658,233 + Pf6N2E2_582 0.77 -1.4 658,233 + Pf6N2E2_582 0.77 -0.6 658,234 - Pf6N2E2_582 0.78 -2.4 658,314 + Pf6N2E2_582 0.85 -1.1 658,315 - Pf6N2E2_582 0.85 -2.3
Or see this region's nucleotide sequence