Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5796

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5794 and Pf6N2E2_5795 are separated by 101 nucleotidesPf6N2E2_5795 and Pf6N2E2_5796 overlap by 1 nucleotidesPf6N2E2_5796 and Pf6N2E2_5797 are separated by 239 nucleotides Pf6N2E2_5794 - Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70), at 6,612,380 to 6,613,015 _5794 Pf6N2E2_5795 - Cell division topological specificity factor MinE, at 6,613,117 to 6,613,371 _5795 Pf6N2E2_5796 - Septum site-determining protein MinD, at 6,613,371 to 6,614,144 _5796 Pf6N2E2_5797 - Septum site-determining protein MinC, at 6,614,384 to 6,615,088 _5797 Position (kb) 6613 6614 6615Strain fitness (log2 ratio) -2 -1 0 1at 6612.381 kb on + strandat 6612.381 kb on + strandat 6612.381 kb on + strandat 6612.381 kb on + strandat 6612.381 kb on + strandat 6612.382 kb on - strandat 6612.750 kb on + strand, within Pf6N2E2_5794at 6612.750 kb on + strand, within Pf6N2E2_5794at 6612.750 kb on + strand, within Pf6N2E2_5794at 6614.292 kb on - strandat 6614.292 kb on - strandat 6614.292 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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6,612,381 + +0.2
6,612,381 + +0.0
6,612,381 + +1.1
6,612,381 + -1.6
6,612,381 + +1.3
6,612,382 - +0.3
6,612,750 + Pf6N2E2_5794 0.58 -1.8
6,612,750 + Pf6N2E2_5794 0.58 +0.1
6,612,750 + Pf6N2E2_5794 0.58 +1.2
6,614,292 - +0.5
6,614,292 - -0.7
6,614,292 - -1.2

Or see this region's nucleotide sequence