Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5770 and Pf6N2E2_5771 are separated by 269 nucleotides Pf6N2E2_5771 and Pf6N2E2_5772 are separated by 294 nucleotides Pf6N2E2_5772 and Pf6N2E2_5773 are separated by 90 nucleotides
Pf6N2E2_5770 - FIG00959155: hypothetical protein, at 6,586,109 to 6,587,914
_5770
Pf6N2E2_5771 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 6,588,184 to 6,588,897
_5771
Pf6N2E2_5772 - DUF1232 domain-containing protein, at 6,589,192 to 6,589,647
_5772
Pf6N2E2_5773 - Transcriptional regulator, Cro/CI family, at 6,589,738 to 6,590,103
_5773
Position (kb)
6588
6589 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 6587.319 kb on + strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.373 kb on + strand, within Pf6N2E2_5770 at 6587.373 kb on + strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.377 kb on + strand, within Pf6N2E2_5770 at 6587.378 kb on - strand, within Pf6N2E2_5770 at 6587.378 kb on - strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.653 kb on - strand, within Pf6N2E2_5770 at 6587.653 kb on - strand, within Pf6N2E2_5770 at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.791 kb on - strand at 6587.791 kb on - strand at 6587.815 kb on - strand at 6587.841 kb on + strand at 6587.841 kb on + strand at 6587.841 kb on + strand at 6587.842 kb on - strand at 6587.842 kb on - strand at 6587.842 kb on - strand at 6587.844 kb on + strand at 6587.844 kb on + strand at 6587.844 kb on + strand at 6587.845 kb on - strand at 6587.845 kb on - strand at 6587.945 kb on + strand at 6587.945 kb on + strand at 6588.017 kb on + strand at 6588.018 kb on - strand at 6588.018 kb on - strand at 6588.021 kb on + strand at 6588.022 kb on - strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.080 kb on - strand at 6588.131 kb on + strand at 6588.132 kb on - strand at 6588.180 kb on + strand at 6588.180 kb on + strand at 6588.180 kb on + strand at 6588.181 kb on - strand at 6588.181 kb on - strand at 6588.230 kb on + strand at 6588.231 kb on - strand at 6588.261 kb on - strand, within Pf6N2E2_5771 at 6588.540 kb on + strand, within Pf6N2E2_5771 at 6588.565 kb on + strand, within Pf6N2E2_5771 at 6588.614 kb on + strand, within Pf6N2E2_5771 at 6588.614 kb on + strand, within Pf6N2E2_5771 at 6588.628 kb on + strand, within Pf6N2E2_5771 at 6588.628 kb on + strand at 6588.629 kb on - strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.761 kb on - strand, within Pf6N2E2_5771 at 6588.761 kb on - strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.895 kb on + strand at 6588.895 kb on + strand at 6588.896 kb on - strand at 6588.896 kb on - strand at 6588.896 kb on - strand at 6588.908 kb on + strand at 6588.908 kb on + strand at 6588.908 kb on + strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.009 kb on + strand at 6589.010 kb on - strand at 6589.023 kb on + strand at 6589.054 kb on + strand at 6589.067 kb on + strand at 6589.104 kb on + strand at 6589.151 kb on + strand at 6589.363 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.430 kb on - strand, within Pf6N2E2_5772 at 6589.430 kb on - strand, within Pf6N2E2_5772 at 6589.461 kb on + strand, within Pf6N2E2_5772 at 6589.461 kb on + strand, within Pf6N2E2_5772 at 6589.462 kb on - strand, within Pf6N2E2_5772 at 6589.700 kb on + strand at 6589.700 kb on - strand at 6589.701 kb on - strand at 6589.701 kb on - strand at 6589.706 kb on + strand at 6589.714 kb on + strand at 6589.715 kb on - strand at 6589.735 kb on + strand at 6589.757 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,587,319 + Pf6N2E2_5770 0.67 -0.6 6,587,320 - Pf6N2E2_5770 0.67 +0.9 6,587,320 - Pf6N2E2_5770 0.67 -1.4 6,587,320 - Pf6N2E2_5770 0.67 -1.3 6,587,370 + Pf6N2E2_5770 0.70 -0.7 6,587,370 + Pf6N2E2_5770 0.70 +1.7 6,587,370 + Pf6N2E2_5770 0.70 +0.7 6,587,370 + Pf6N2E2_5770 0.70 -2.5 6,587,371 - Pf6N2E2_5770 0.70 -0.1 6,587,371 - Pf6N2E2_5770 0.70 -0.8 6,587,371 - Pf6N2E2_5770 0.70 +0.4 6,587,371 - Pf6N2E2_5770 0.70 -0.8 6,587,371 - Pf6N2E2_5770 0.70 +1.0 6,587,371 - Pf6N2E2_5770 0.70 -2.4 6,587,373 + Pf6N2E2_5770 0.70 +0.6 6,587,373 + Pf6N2E2_5770 0.70 -1.6 6,587,374 - Pf6N2E2_5770 0.70 +0.2 6,587,374 - Pf6N2E2_5770 0.70 +0.8 6,587,374 - Pf6N2E2_5770 0.70 -0.7 6,587,374 - Pf6N2E2_5770 0.70 +0.1 6,587,377 + Pf6N2E2_5770 0.70 +1.4 6,587,378 - Pf6N2E2_5770 0.70 -1.4 6,587,378 - Pf6N2E2_5770 0.70 +1.0 6,587,428 + Pf6N2E2_5770 0.73 +0.1 6,587,428 + Pf6N2E2_5770 0.73 -0.3 6,587,428 + Pf6N2E2_5770 0.73 +0.6 6,587,428 + Pf6N2E2_5770 0.73 -1.6 6,587,428 + Pf6N2E2_5770 0.73 -2.8 6,587,428 + Pf6N2E2_5770 0.73 -1.4 6,587,429 - Pf6N2E2_5770 0.73 -0.3 6,587,429 - Pf6N2E2_5770 0.73 +3.8 6,587,429 - Pf6N2E2_5770 0.73 +1.1 6,587,429 - Pf6N2E2_5770 0.73 +0.8 6,587,429 - Pf6N2E2_5770 0.73 -0.1 6,587,653 - Pf6N2E2_5770 0.85 -0.7 6,587,653 - Pf6N2E2_5770 0.85 +1.7 6,587,751 + +1.4 6,587,751 + +1.3 6,587,751 + -2.3 6,587,751 + +0.7 6,587,751 + +1.0 6,587,752 - -1.5 6,587,752 - -0.7 6,587,752 - +0.6 6,587,752 - -0.5 6,587,752 - -0.7 6,587,754 + -0.8 6,587,754 + +1.6 6,587,754 + -0.6 6,587,754 + -1.2 6,587,755 - +0.8 6,587,755 - +1.9 6,587,755 - -0.0 6,587,755 - -1.5 6,587,755 - -1.3 6,587,791 - -0.5 6,587,791 - +2.2 6,587,815 - -1.8 6,587,841 + +0.2 6,587,841 + +0.4 6,587,841 + -1.0 6,587,842 - -0.2 6,587,842 - +1.4 6,587,842 - -1.9 6,587,844 + +0.9 6,587,844 + -0.4 6,587,844 + +0.4 6,587,845 - -2.1 6,587,845 - +0.7 6,587,945 + +1.1 6,587,945 + -3.0 6,588,017 + +2.1 6,588,018 - -2.4 6,588,018 - -2.0 6,588,021 + +0.3 6,588,022 - -0.6 6,588,079 + -1.0 6,588,079 + -0.1 6,588,079 + -0.7 6,588,079 + -1.1 6,588,079 + -0.0 6,588,079 + -0.5 6,588,080 - +0.3 6,588,131 + -1.2 6,588,132 - -2.2 6,588,180 + -0.3 6,588,180 + -3.4 6,588,180 + -1.3 6,588,181 - -2.6 6,588,181 - -1.7 6,588,230 + -3.1 6,588,231 - -2.0 6,588,261 - Pf6N2E2_5771 0.11 -2.1 6,588,540 + Pf6N2E2_5771 0.50 -3.4 6,588,565 + Pf6N2E2_5771 0.53 -2.7 6,588,614 + Pf6N2E2_5771 0.60 -2.4 6,588,614 + Pf6N2E2_5771 0.60 -2.8 6,588,628 + Pf6N2E2_5771 0.62 -2.2 6,588,628 + +1.3 6,588,629 - Pf6N2E2_5771 0.62 -2.1 6,588,760 + Pf6N2E2_5771 0.81 -3.0 6,588,760 + Pf6N2E2_5771 0.81 -2.9 6,588,760 + Pf6N2E2_5771 0.81 -2.3 6,588,760 + Pf6N2E2_5771 0.81 +0.3 6,588,760 + Pf6N2E2_5771 0.81 -1.6 6,588,760 + Pf6N2E2_5771 0.81 -2.7 6,588,761 - Pf6N2E2_5771 0.81 -0.6 6,588,761 - Pf6N2E2_5771 0.81 -1.2 6,588,769 + Pf6N2E2_5771 0.82 -3.4 6,588,769 + Pf6N2E2_5771 0.82 -2.6 6,588,769 + Pf6N2E2_5771 0.82 -1.2 6,588,769 + Pf6N2E2_5771 0.82 -2.9 6,588,769 + Pf6N2E2_5771 0.82 -1.5 6,588,769 + Pf6N2E2_5771 0.82 -1.9 6,588,895 + -2.1 6,588,895 + -1.4 6,588,896 - -0.5 6,588,896 - -4.1 6,588,896 - -0.7 6,588,908 + -3.1 6,588,908 + -1.0 6,588,908 + -2.0 6,588,909 - -1.6 6,588,909 - -0.8 6,588,909 - -0.2 6,588,909 - -1.4 6,588,909 - -2.9 6,588,995 - +0.4 6,588,995 - -1.2 6,588,995 - -0.6 6,588,995 - +0.1 6,588,995 - -1.0 6,588,996 + -0.1 6,588,996 + +0.4 6,588,996 + +1.7 6,588,996 + -0.2 6,588,996 + -1.4 6,588,997 - -0.2 6,588,997 - -1.8 6,588,997 - -1.0 6,588,997 - -1.5 6,589,003 - -2.2 6,589,003 - -0.5 6,589,003 - -1.2 6,589,003 - -0.4 6,589,003 - -0.4 6,589,009 + -1.4 6,589,010 - -1.6 6,589,023 + -2.0 6,589,054 + -0.4 6,589,067 + -0.9 6,589,104 + -1.8 6,589,151 + -2.2 6,589,363 + Pf6N2E2_5772 0.38 -0.2 6,589,429 + Pf6N2E2_5772 0.52 +0.8 6,589,429 + Pf6N2E2_5772 0.52 -2.7 6,589,429 + Pf6N2E2_5772 0.52 +1.5 6,589,430 - Pf6N2E2_5772 0.52 +2.0 6,589,430 - Pf6N2E2_5772 0.52 +0.2 6,589,461 + Pf6N2E2_5772 0.59 -1.1 6,589,461 + Pf6N2E2_5772 0.59 -1.1 6,589,462 - Pf6N2E2_5772 0.59 +0.3 6,589,700 + -0.1 6,589,700 - -0.4 6,589,701 - -1.4 6,589,701 - -0.8 6,589,706 + +0.9 6,589,714 + -0.4 6,589,715 - +1.2 6,589,735 + +0.5 6,589,757 + +0.9
Or see this region's nucleotide sequence