Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5697

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
6,508,328	+		Pf6N2E2_5695	0.50	-2.9
6,508,411	+		Pf6N2E2_5695	0.69	-3.0
6,508,411	+		Pf6N2E2_5695	0.69	-2.1
6,508,411	+		Pf6N2E2_5695	0.69	-3.1
6,508,411	+		Pf6N2E2_5695	0.69	-2.4
6,508,411	+		Pf6N2E2_5695	0.69	-2.9
6,508,411	+		Pf6N2E2_5695	0.69	-3.5
6,508,412	-		Pf6N2E2_5695	0.69	-4.0
6,508,412	-		Pf6N2E2_5695	0.69	-3.3
6,508,412	-		Pf6N2E2_5695	0.69	-0.9
6,508,417	+		Pf6N2E2_5695	0.70	-3.2
6,508,418	-		Pf6N2E2_5695	0.70	-1.3
6,508,418	-		Pf6N2E2_5695	0.70	-1.4
6,508,418	-		Pf6N2E2_5695	0.70	-3.9
6,508,418	-		Pf6N2E2_5695	0.70	-2.9
6,508,418	-		Pf6N2E2_5695	0.70	-3.4
6,508,418	-		Pf6N2E2_5695	0.70	-2.4
6,508,549	+				-2.0
6,508,581	+				-2.8
6,508,581	+				-3.8
6,508,852	+		Pf6N2E2_5696	0.40	-2.0
6,508,852	+		Pf6N2E2_5696	0.40	-3.4
6,508,852	+		Pf6N2E2_5696	0.40	-3.5
6,508,853	-		Pf6N2E2_5696	0.40	-2.2
6,508,853	-		Pf6N2E2_5696	0.40	-1.3
6,508,932	+		Pf6N2E2_5696	0.51	-2.0
6,509,116	+		Pf6N2E2_5696	0.76	+0.9
6,509,116	+		Pf6N2E2_5696	0.76	-1.1
6,509,116	+		Pf6N2E2_5696	0.76	+1.2
6,509,116	+		Pf6N2E2_5696	0.76	+0.8
6,509,116	+		Pf6N2E2_5696	0.76	-0.3
6,509,116	+		Pf6N2E2_5696	0.76	-0.8
6,509,117	-		Pf6N2E2_5696	0.76	-2.3
6,509,117	-		Pf6N2E2_5696	0.76	-1.1
6,509,117	-		Pf6N2E2_5696	0.76	-3.2
6,509,117	-		Pf6N2E2_5696	0.76	-2.7
6,509,117	-		Pf6N2E2_5696	0.76	-3.8
6,509,274	+				+0.1
6,509,274	+				-0.3
6,509,274	+				+0.6
6,509,275	-				-2.5
6,509,275	-				-3.3
6,509,280	+				-2.1
6,509,280	+				-2.2
6,509,280	+				-2.2
6,509,280	+				-0.6
6,509,280	+				-1.9
6,509,280	+				-2.1
6,509,281	-				-3.7
6,509,281	-				-1.2
6,509,281	-				-1.6
6,509,284	+				-0.5
6,509,284	+				+0.0
6,509,284	+				+0.1
6,509,284	+				-0.8
6,509,284	+				+0.5
6,509,284	+				+0.5
6,509,284	+				+1.6
6,509,284	+				-0.2
6,509,284	+				+0.3
6,509,285	-				-3.5
6,509,285	-				-2.2
6,509,345	+				+0.3
6,509,441	+				-3.1
6,509,442	-				-2.0
6,509,442	-				-1.9
6,509,612	+		Pf6N2E2_5697	0.22	+0.2
6,509,612	+		Pf6N2E2_5697	0.22	-1.4
6,509,613	-		Pf6N2E2_5697	0.22	-2.2
6,509,613	-		Pf6N2E2_5697	0.22	-3.5
6,509,613	-		Pf6N2E2_5697	0.22	-0.8
6,509,671	+		Pf6N2E2_5697	0.27	-1.1
6,509,672	-		Pf6N2E2_5697	0.27	-2.7
6,509,690	+		Pf6N2E2_5697	0.28	-3.0
6,509,691	-		Pf6N2E2_5697	0.28	+0.2
6,509,885	+		Pf6N2E2_5697	0.43	-1.9
6,509,885	+		Pf6N2E2_5697	0.43	-1.9
6,509,921	+		Pf6N2E2_5697	0.46	-2.7
6,509,921	+		Pf6N2E2_5697	0.46	-2.8
6,509,921	+		Pf6N2E2_5697	0.46	+2.7
6,509,921	+		Pf6N2E2_5697	0.46	-3.9
6,509,921	+		Pf6N2E2_5697	0.46	-2.3
6,509,922	-		Pf6N2E2_5697	0.46	-1.9
6,509,922	-		Pf6N2E2_5697	0.46	+0.3
6,509,922	-		Pf6N2E2_5697	0.46	-1.5
6,509,922	-		Pf6N2E2_5697	0.46	-3.8
6,509,922	-		Pf6N2E2_5697	0.46	-4.1
6,509,944	+		Pf6N2E2_5697	0.47	-1.9
6,509,944	+		Pf6N2E2_5697	0.47	-1.5
6,509,957	+		Pf6N2E2_5697	0.48	-0.9
6,509,957	+		Pf6N2E2_5697	0.48	-3.3
6,509,957	+		Pf6N2E2_5697	0.48	-1.0
6,509,957	+		Pf6N2E2_5697	0.48	-1.1
6,509,957	+		Pf6N2E2_5697	0.48	-2.5
6,509,957	+		Pf6N2E2_5697	0.48	-3.2
6,509,957	+		Pf6N2E2_5697	0.48	-3.6
6,509,957	+		Pf6N2E2_5697	0.48	-2.5
6,509,958	-		Pf6N2E2_5697	0.48	-2.0
6,509,958	-		Pf6N2E2_5697	0.48	-2.3
6,509,958	-		Pf6N2E2_5697	0.48	-2.5
6,509,959	+		Pf6N2E2_5697	0.49	-3.4
6,509,959	+		Pf6N2E2_5697	0.49	-2.4
6,509,959	+		Pf6N2E2_5697	0.49	-1.7
6,509,959	+		Pf6N2E2_5697	0.49	-1.9
6,509,959	+		Pf6N2E2_5697	0.49	-2.2
6,509,960	-		Pf6N2E2_5697	0.49	-2.7
6,509,960	-		Pf6N2E2_5697	0.49	-1.3
6,509,960	-		Pf6N2E2_5697	0.49	-2.2
6,509,960	-		Pf6N2E2_5697	0.49	-1.2
6,509,960	-		Pf6N2E2_5697	0.49	-4.5
6,509,960	-		Pf6N2E2_5697	0.49	-2.5
6,509,977	+		Pf6N2E2_5697	0.50	-3.0
6,510,166	+		Pf6N2E2_5697	0.64	-1.1
6,510,166	+		Pf6N2E2_5697	0.64	-0.8
6,510,166	+		Pf6N2E2_5697	0.64	-2.4
6,510,166	+		Pf6N2E2_5697	0.64	+0.4
6,510,167	-		Pf6N2E2_5697	0.64	-1.3
6,510,167	-		Pf6N2E2_5697	0.64	-3.0
6,510,172	+		Pf6N2E2_5697	0.65	-1.2
6,510,172	+		Pf6N2E2_5697	0.65	-2.7
6,510,172	+		Pf6N2E2_5697	0.65	-2.4
6,510,172	+		Pf6N2E2_5697	0.65	-0.4
6,510,173	-		Pf6N2E2_5697	0.65	-1.5
6,510,185	-		Pf6N2E2_5697	0.66	-2.5
6,510,205	+		Pf6N2E2_5697	0.67	-0.6
6,510,205	+		Pf6N2E2_5697	0.67	-2.4
6,510,205	+		Pf6N2E2_5697	0.67	-2.6
6,510,206	-		Pf6N2E2_5697	0.67	-2.8
6,510,208	+		Pf6N2E2_5697	0.67	-1.1
6,510,209	-		Pf6N2E2_5697	0.67	-2.9
6,510,209	-		Pf6N2E2_5697	0.67	-3.2
6,510,209	-		Pf6N2E2_5697	0.67	-2.1
6,510,209	-		Pf6N2E2_5697	0.67	-1.4
6,510,209	-		Pf6N2E2_5697	0.67	-1.7
6,510,296	-		Pf6N2E2_5697	0.74	-2.2
6,510,296	-		Pf6N2E2_5697	0.74	-1.5
6,510,296	-		Pf6N2E2_5697	0.74	-0.0
6,510,296	-		Pf6N2E2_5697	0.74	-2.9
6,510,296	-		Pf6N2E2_5697	0.74	-3.6
6,510,324	+		Pf6N2E2_5697	0.76	-3.2
6,510,324	+		Pf6N2E2_5697	0.76	-2.7
6,510,324	+		Pf6N2E2_5697	0.76	-3.3
6,510,324	+		Pf6N2E2_5697	0.76	-3.1
6,510,324	+		Pf6N2E2_5697	0.76	-3.3
6,510,324	+		Pf6N2E2_5697	0.76	-3.4
6,510,324	+		Pf6N2E2_5697	0.76	-3.5
6,510,325	-		Pf6N2E2_5697	0.76	-3.0
6,510,325	-		Pf6N2E2_5697	0.76	-2.1
6,510,325	-		Pf6N2E2_5697	0.76	-2.6
6,510,325	-		Pf6N2E2_5697	0.76	-2.9
6,510,325	-		Pf6N2E2_5697	0.76	-2.3
6,510,325	-		Pf6N2E2_5697	0.76	-2.8
6,510,325	-		Pf6N2E2_5697	0.76	-1.3
6,510,325	-		Pf6N2E2_5697	0.76	-2.9
6,510,325	-		Pf6N2E2_5697	0.76	-2.1
6,510,325	-		Pf6N2E2_5697	0.76	-3.0
6,510,572	+				-4.0
6,510,572	+				-2.2
6,510,572	+				-1.8
6,510,572	+				-3.7
6,510,573	-				-3.7
6,510,573	-				-1.5
6,510,573	-				-3.1
6,510,573	-				-1.9
6,510,704	-				+0.5
6,510,749	-				-0.7
6,510,791	+				+0.2
6,510,791	+				+1.6
6,510,792	-				+1.4
6,510,792	-				+1.4
6,510,886	+				-0.5
6,510,886	+				-1.2
6,510,983	-				-0.1
6,510,983	-				-2.8