Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6161 and Pf6N2E2_5684 are separated by 427 nucleotides Pf6N2E2_5684 and Pf6N2E2_5685 overlap by 8 nucleotides Pf6N2E2_5685 and Pf6N2E2_5686 are separated by 159 nucleotides
Pf6N2E2_6161 - tRNA-Arg-CCT, at 6,497,098 to 6,497,171
_6161
Pf6N2E2_5684 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 6,497,599 to 6,499,206
_5684
Pf6N2E2_5685 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 6,499,199 to 6,499,891
_5685
Pf6N2E2_5686 - glutamine synthetase family protein, at 6,500,051 to 6,501,394
_5686
Position (kb)
6497
6498
6499
6500 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 6496.628 kb on + strand at 6496.629 kb on - strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.828 kb on + strand at 6496.829 kb on - strand at 6496.891 kb on - strand at 6496.917 kb on - strand at 6496.919 kb on + strand at 6496.983 kb on + strand at 6496.983 kb on + strand at 6496.984 kb on - strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.993 kb on + strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.994 kb on - strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.996 kb on + strand at 6496.997 kb on - strand at 6496.997 kb on - strand at 6497.005 kb on - strand at 6497.005 kb on - strand at 6497.005 kb on - strand at 6497.011 kb on - strand at 6497.011 kb on - strand at 6497.175 kb on + strand at 6497.178 kb on + strand at 6497.179 kb on - strand at 6497.238 kb on + strand at 6497.238 kb on + strand at 6497.239 kb on - strand at 6497.516 kb on - strand at 6497.559 kb on - strand at 6497.580 kb on - strand at 6497.609 kb on - strand at 6497.752 kb on + strand at 6497.752 kb on + strand at 6497.752 kb on + strand at 6497.752 kb on + strand at 6497.773 kb on + strand, within Pf6N2E2_5684 at 6497.773 kb on + strand, within Pf6N2E2_5684 at 6497.773 kb on + strand, within Pf6N2E2_5684 at 6497.774 kb on - strand, within Pf6N2E2_5684 at 6497.774 kb on - strand, within Pf6N2E2_5684 at 6497.774 kb on - strand, within Pf6N2E2_5684 at 6497.774 kb on - strand, within Pf6N2E2_5684 at 6497.774 kb on - strand, within Pf6N2E2_5684 at 6497.927 kb on - strand, within Pf6N2E2_5684 at 6498.160 kb on + strand, within Pf6N2E2_5684 at 6498.160 kb on + strand, within Pf6N2E2_5684 at 6498.160 kb on + strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.161 kb on - strand, within Pf6N2E2_5684 at 6498.301 kb on + strand, within Pf6N2E2_5684 at 6498.302 kb on - strand, within Pf6N2E2_5684 at 6498.302 kb on - strand, within Pf6N2E2_5684 at 6498.443 kb on - strand, within Pf6N2E2_5684 at 6498.791 kb on + strand, within Pf6N2E2_5684 at 6498.791 kb on + strand, within Pf6N2E2_5684 at 6498.792 kb on - strand, within Pf6N2E2_5684 at 6498.850 kb on + strand, within Pf6N2E2_5684 at 6498.850 kb on + strand, within Pf6N2E2_5684 at 6498.961 kb on + strand, within Pf6N2E2_5684 at 6498.961 kb on + strand, within Pf6N2E2_5684 at 6499.035 kb on + strand, within Pf6N2E2_5684 at 6499.035 kb on + strand, within Pf6N2E2_5684 at 6499.036 kb on - strand, within Pf6N2E2_5684 at 6499.036 kb on - strand, within Pf6N2E2_5684 at 6499.294 kb on + strand, within Pf6N2E2_5685 at 6499.294 kb on + strand, within Pf6N2E2_5685 at 6499.294 kb on + strand, within Pf6N2E2_5685 at 6499.295 kb on - strand, within Pf6N2E2_5685 at 6499.406 kb on + strand, within Pf6N2E2_5685 at 6499.406 kb on + strand, within Pf6N2E2_5685 at 6499.406 kb on + strand, within Pf6N2E2_5685 at 6499.407 kb on - strand, within Pf6N2E2_5685 at 6499.446 kb on - strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.526 kb on + strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.527 kb on - strand, within Pf6N2E2_5685 at 6499.604 kb on - strand, within Pf6N2E2_5685 at 6499.604 kb on - strand, within Pf6N2E2_5685 at 6499.604 kb on - strand, within Pf6N2E2_5685 at 6499.730 kb on + strand, within Pf6N2E2_5685 at 6499.778 kb on + strand, within Pf6N2E2_5685 at 6499.778 kb on + strand, within Pf6N2E2_5685 at 6499.778 kb on + strand, within Pf6N2E2_5685 at 6499.778 kb on + strand, within Pf6N2E2_5685 at 6499.779 kb on - strand, within Pf6N2E2_5685 at 6499.779 kb on - strand, within Pf6N2E2_5685 at 6499.880 kb on + strand at 6499.880 kb on + strand at 6499.880 kb on + strand at 6499.880 kb on + strand at 6499.881 kb on - strand at 6499.881 kb on - strand at 6499.881 kb on - strand at 6499.903 kb on + strand at 6499.907 kb on + strand at 6499.907 kb on + strand at 6499.907 kb on + strand at 6499.907 kb on + strand at 6499.907 kb on + strand at 6499.907 kb on + strand at 6499.961 kb on + strand at 6499.961 kb on + strand at 6499.961 kb on + strand at 6499.961 kb on + strand at 6499.961 kb on + strand at 6499.961 kb on + strand at 6499.962 kb on - strand at 6499.962 kb on - strand at 6499.982 kb on - strand at 6499.982 kb on - strand at 6500.001 kb on + strand at 6500.001 kb on + strand at 6500.001 kb on + strand at 6500.002 kb on - strand at 6500.002 kb on - strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.035 kb on + strand at 6500.036 kb on - strand at 6500.036 kb on - strand at 6500.036 kb on - strand at 6500.036 kb on - strand at 6500.036 kb on - strand at 6500.151 kb on + strand at 6500.152 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,496,628 + -1.5 6,496,629 - +0.7 6,496,828 + -1.6 6,496,828 + -0.5 6,496,828 + -0.5 6,496,828 + -0.8 6,496,828 + +0.9 6,496,829 - +1.2 6,496,891 - +1.7 6,496,917 - +1.6 6,496,919 + -2.0 6,496,983 + -0.8 6,496,983 + -1.4 6,496,984 - -1.5 6,496,993 + -0.4 6,496,993 + -1.4 6,496,993 + -0.8 6,496,993 + +0.0 6,496,994 - +2.1 6,496,994 - +0.6 6,496,994 - +0.1 6,496,994 - +1.0 6,496,996 + -0.2 6,496,996 + -0.8 6,496,996 + -2.2 6,496,996 + -0.2 6,496,996 + -2.2 6,496,996 + -1.6 6,496,996 + -0.3 6,496,997 - -0.4 6,496,997 - +2.2 6,497,005 - -1.1 6,497,005 - +2.7 6,497,005 - +0.7 6,497,011 - +2.8 6,497,011 - +1.6 6,497,175 + +1.9 6,497,178 + -1.4 6,497,179 - +1.8 6,497,238 + -0.4 6,497,238 + -0.3 6,497,239 - +1.1 6,497,516 - -0.8 6,497,559 - -0.1 6,497,580 - -0.9 6,497,609 - -0.3 6,497,752 + -0.4 6,497,752 + -0.4 6,497,752 + -1.2 6,497,752 + -0.2 6,497,773 + Pf6N2E2_5684 0.11 -1.9 6,497,773 + Pf6N2E2_5684 0.11 -1.0 6,497,773 + Pf6N2E2_5684 0.11 -2.6 6,497,774 - Pf6N2E2_5684 0.11 -0.9 6,497,774 - Pf6N2E2_5684 0.11 -0.2 6,497,774 - Pf6N2E2_5684 0.11 -2.1 6,497,774 - Pf6N2E2_5684 0.11 +0.4 6,497,774 - Pf6N2E2_5684 0.11 -0.6 6,497,927 - Pf6N2E2_5684 0.20 -0.9 6,498,160 + Pf6N2E2_5684 0.35 -0.1 6,498,160 + Pf6N2E2_5684 0.35 -1.0 6,498,160 + Pf6N2E2_5684 0.35 -1.5 6,498,161 - Pf6N2E2_5684 0.35 -0.7 6,498,161 - Pf6N2E2_5684 0.35 -2.0 6,498,161 - Pf6N2E2_5684 0.35 -0.3 6,498,161 - Pf6N2E2_5684 0.35 -0.1 6,498,161 - Pf6N2E2_5684 0.35 -0.6 6,498,161 - Pf6N2E2_5684 0.35 -1.2 6,498,301 + Pf6N2E2_5684 0.44 -2.3 6,498,302 - Pf6N2E2_5684 0.44 -0.3 6,498,302 - Pf6N2E2_5684 0.44 -0.3 6,498,443 - Pf6N2E2_5684 0.52 -2.0 6,498,791 + Pf6N2E2_5684 0.74 -1.5 6,498,791 + Pf6N2E2_5684 0.74 -1.5 6,498,792 - Pf6N2E2_5684 0.74 +0.2 6,498,850 + Pf6N2E2_5684 0.78 -0.9 6,498,850 + Pf6N2E2_5684 0.78 -2.7 6,498,961 + Pf6N2E2_5684 0.85 -0.1 6,498,961 + Pf6N2E2_5684 0.85 -1.5 6,499,035 + Pf6N2E2_5684 0.89 +0.2 6,499,035 + Pf6N2E2_5684 0.89 -1.9 6,499,036 - Pf6N2E2_5684 0.89 -2.4 6,499,036 - Pf6N2E2_5684 0.89 -3.8 6,499,294 + Pf6N2E2_5685 0.14 -1.9 6,499,294 + Pf6N2E2_5685 0.14 -0.2 6,499,294 + Pf6N2E2_5685 0.14 -2.3 6,499,295 - Pf6N2E2_5685 0.14 +0.9 6,499,406 + Pf6N2E2_5685 0.30 -1.7 6,499,406 + Pf6N2E2_5685 0.30 -1.5 6,499,406 + Pf6N2E2_5685 0.30 -1.4 6,499,407 - Pf6N2E2_5685 0.30 -0.8 6,499,446 - Pf6N2E2_5685 0.36 -0.6 6,499,526 + Pf6N2E2_5685 0.47 -0.3 6,499,526 + Pf6N2E2_5685 0.47 -1.3 6,499,526 + Pf6N2E2_5685 0.47 -0.3 6,499,526 + Pf6N2E2_5685 0.47 -3.4 6,499,526 + Pf6N2E2_5685 0.47 -1.0 6,499,526 + Pf6N2E2_5685 0.47 -0.3 6,499,527 - Pf6N2E2_5685 0.47 -1.4 6,499,527 - Pf6N2E2_5685 0.47 -1.5 6,499,527 - Pf6N2E2_5685 0.47 -2.7 6,499,527 - Pf6N2E2_5685 0.47 -1.9 6,499,527 - Pf6N2E2_5685 0.47 -1.3 6,499,527 - Pf6N2E2_5685 0.47 -1.8 6,499,604 - Pf6N2E2_5685 0.58 -1.2 6,499,604 - Pf6N2E2_5685 0.58 -2.0 6,499,604 - Pf6N2E2_5685 0.58 -1.5 6,499,730 + Pf6N2E2_5685 0.77 -0.4 6,499,778 + Pf6N2E2_5685 0.84 +0.0 6,499,778 + Pf6N2E2_5685 0.84 -1.1 6,499,778 + Pf6N2E2_5685 0.84 -1.6 6,499,778 + Pf6N2E2_5685 0.84 -3.0 6,499,779 - Pf6N2E2_5685 0.84 -3.8 6,499,779 - Pf6N2E2_5685 0.84 -2.8 6,499,880 + -2.9 6,499,880 + -2.6 6,499,880 + -1.7 6,499,880 + -0.4 6,499,881 - -2.4 6,499,881 - +0.3 6,499,881 - -1.7 6,499,903 + +1.0 6,499,907 + +0.2 6,499,907 + +5.2 6,499,907 + -0.9 6,499,907 + +0.4 6,499,907 + -0.3 6,499,907 + -0.8 6,499,961 + -1.2 6,499,961 + -0.3 6,499,961 + -1.9 6,499,961 + -1.0 6,499,961 + -0.4 6,499,961 + -1.4 6,499,962 - -1.9 6,499,962 - -0.5 6,499,982 - -0.2 6,499,982 - +1.4 6,500,001 + -0.4 6,500,001 + +0.7 6,500,001 + +0.5 6,500,002 - +0.5 6,500,002 - -1.2 6,500,035 + -3.0 6,500,035 + -0.9 6,500,035 + +1.1 6,500,035 + -1.9 6,500,035 + +1.4 6,500,035 + -0.4 6,500,035 + -0.3 6,500,035 + +0.6 6,500,035 + -0.3 6,500,035 + -1.7 6,500,036 - +0.0 6,500,036 - -0.8 6,500,036 - -1.2 6,500,036 - +0.1 6,500,036 - +0.0 6,500,151 + -0.3 6,500,152 - -1.5
Or see this region's nucleotide sequence