Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5588 and Pf6N2E2_5589 are separated by 190 nucleotides Pf6N2E2_5589 and Pf6N2E2_5590 are separated by 129 nucleotides
Pf6N2E2_5588 - Dihydroorotase (EC 3.5.2.3), at 6,405,133 to 6,406,179
_5588
Pf6N2E2_5589 - Sodium-type flagellar protein motY precursor, at 6,406,370 to 6,407,347
_5589
Pf6N2E2_5590 - Argininosuccinate synthase (EC 6.3.4.5), at 6,407,477 to 6,408,694
_5590
Position (kb)
6406
6407
6408 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6406.265 kb on + strand at 6406.265 kb on + strand at 6406.266 kb on - strand at 6406.271 kb on + strand at 6406.278 kb on + strand at 6406.278 kb on + strand at 6406.278 kb on + strand at 6406.279 kb on - strand at 6406.279 kb on - strand at 6406.347 kb on + strand at 6406.348 kb on - strand at 6406.349 kb on + strand at 6406.349 kb on + strand at 6406.349 kb on + strand at 6406.349 kb on + strand at 6406.349 kb on + strand at 6406.350 kb on - strand at 6406.350 kb on - strand at 6406.382 kb on + strand at 6406.382 kb on + strand at 6406.386 kb on + strand at 6406.386 kb on + strand at 6406.386 kb on + strand at 6406.386 kb on + strand at 6406.386 kb on + strand at 6406.387 kb on - strand at 6406.387 kb on - strand at 6406.387 kb on - strand at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.574 kb on + strand, within Pf6N2E2_5589 at 6406.575 kb on - strand, within Pf6N2E2_5589 at 6406.575 kb on - strand, within Pf6N2E2_5589 at 6406.576 kb on + strand, within Pf6N2E2_5589 at 6406.576 kb on + strand, within Pf6N2E2_5589 at 6406.577 kb on - strand, within Pf6N2E2_5589 at 6406.577 kb on - strand, within Pf6N2E2_5589 at 6406.758 kb on + strand, within Pf6N2E2_5589 at 6406.759 kb on - strand, within Pf6N2E2_5589 at 6407.158 kb on + strand, within Pf6N2E2_5589 at 6407.159 kb on - strand, within Pf6N2E2_5589 at 6407.159 kb on - strand, within Pf6N2E2_5589 at 6407.378 kb on - strand at 6407.378 kb on - strand at 6407.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 6,406,265 + -1.2 6,406,265 + -0.8 6,406,266 - -2.0 6,406,271 + +0.1 6,406,278 + -0.6 6,406,278 + -0.9 6,406,278 + -0.7 6,406,279 - -3.0 6,406,279 - -0.5 6,406,347 + -1.0 6,406,348 - -2.0 6,406,349 + +0.9 6,406,349 + +0.8 6,406,349 + -1.5 6,406,349 + +0.1 6,406,349 + -0.8 6,406,350 - -2.0 6,406,350 - -1.1 6,406,382 + +0.7 6,406,382 + -0.8 6,406,386 + -0.8 6,406,386 + -3.0 6,406,386 + -3.0 6,406,386 + -2.2 6,406,386 + -2.7 6,406,387 - -2.7 6,406,387 - -1.5 6,406,387 - -1.9 6,406,574 + Pf6N2E2_5589 0.21 -2.7 6,406,574 + Pf6N2E2_5589 0.21 -2.7 6,406,574 + Pf6N2E2_5589 0.21 -1.5 6,406,574 + Pf6N2E2_5589 0.21 -2.6 6,406,575 - Pf6N2E2_5589 0.21 -2.7 6,406,575 - Pf6N2E2_5589 0.21 -3.3 6,406,576 + Pf6N2E2_5589 0.21 -3.4 6,406,576 + Pf6N2E2_5589 0.21 -2.0 6,406,577 - Pf6N2E2_5589 0.21 -2.1 6,406,577 - Pf6N2E2_5589 0.21 -1.3 6,406,758 + Pf6N2E2_5589 0.40 -3.1 6,406,759 - Pf6N2E2_5589 0.40 -4.1 6,407,158 + Pf6N2E2_5589 0.81 -3.3 6,407,159 - Pf6N2E2_5589 0.81 -2.7 6,407,159 - Pf6N2E2_5589 0.81 -2.2 6,407,378 - +0.5 6,407,378 - -2.4 6,407,398 + +1.2
Or see this region's nucleotide sequence