Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5589

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5588 and Pf6N2E2_5589 are separated by 190 nucleotidesPf6N2E2_5589 and Pf6N2E2_5590 are separated by 129 nucleotides Pf6N2E2_5588 - Dihydroorotase (EC 3.5.2.3), at 6,405,133 to 6,406,179 _5588 Pf6N2E2_5589 - Sodium-type flagellar protein motY precursor, at 6,406,370 to 6,407,347 _5589 Pf6N2E2_5590 - Argininosuccinate synthase (EC 6.3.4.5), at 6,407,477 to 6,408,694 _5590 Position (kb) 6406 6407 6408Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 6406.265 kb on + strandat 6406.265 kb on + strandat 6406.266 kb on - strandat 6406.271 kb on + strandat 6406.278 kb on + strandat 6406.278 kb on + strandat 6406.278 kb on + strandat 6406.279 kb on - strandat 6406.279 kb on - strandat 6406.347 kb on + strandat 6406.348 kb on - strandat 6406.349 kb on + strandat 6406.349 kb on + strandat 6406.349 kb on + strandat 6406.349 kb on + strandat 6406.349 kb on + strandat 6406.350 kb on - strandat 6406.350 kb on - strandat 6406.382 kb on + strandat 6406.382 kb on + strandat 6406.386 kb on + strandat 6406.386 kb on + strandat 6406.386 kb on + strandat 6406.386 kb on + strandat 6406.386 kb on + strandat 6406.387 kb on - strandat 6406.387 kb on - strandat 6406.387 kb on - strandat 6406.574 kb on + strand, within Pf6N2E2_5589at 6406.574 kb on + strand, within Pf6N2E2_5589at 6406.574 kb on + strand, within Pf6N2E2_5589at 6406.574 kb on + strand, within Pf6N2E2_5589at 6406.575 kb on - strand, within Pf6N2E2_5589at 6406.575 kb on - strand, within Pf6N2E2_5589at 6406.576 kb on + strand, within Pf6N2E2_5589at 6406.576 kb on + strand, within Pf6N2E2_5589at 6406.577 kb on - strand, within Pf6N2E2_5589at 6406.577 kb on - strand, within Pf6N2E2_5589at 6406.758 kb on + strand, within Pf6N2E2_5589at 6406.759 kb on - strand, within Pf6N2E2_5589at 6407.158 kb on + strand, within Pf6N2E2_5589at 6407.159 kb on - strand, within Pf6N2E2_5589at 6407.159 kb on - strand, within Pf6N2E2_5589at 6407.378 kb on - strandat 6407.378 kb on - strandat 6407.398 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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6,406,265 + -1.2
6,406,265 + -0.8
6,406,266 - -2.0
6,406,271 + +0.1
6,406,278 + -0.6
6,406,278 + -0.9
6,406,278 + -0.7
6,406,279 - -3.0
6,406,279 - -0.5
6,406,347 + -1.0
6,406,348 - -2.0
6,406,349 + +0.9
6,406,349 + +0.8
6,406,349 + -1.5
6,406,349 + +0.1
6,406,349 + -0.8
6,406,350 - -2.0
6,406,350 - -1.1
6,406,382 + +0.7
6,406,382 + -0.8
6,406,386 + -0.8
6,406,386 + -3.0
6,406,386 + -3.0
6,406,386 + -2.2
6,406,386 + -2.7
6,406,387 - -2.7
6,406,387 - -1.5
6,406,387 - -1.9
6,406,574 + Pf6N2E2_5589 0.21 -2.7
6,406,574 + Pf6N2E2_5589 0.21 -2.7
6,406,574 + Pf6N2E2_5589 0.21 -1.5
6,406,574 + Pf6N2E2_5589 0.21 -2.6
6,406,575 - Pf6N2E2_5589 0.21 -2.7
6,406,575 - Pf6N2E2_5589 0.21 -3.3
6,406,576 + Pf6N2E2_5589 0.21 -3.4
6,406,576 + Pf6N2E2_5589 0.21 -2.0
6,406,577 - Pf6N2E2_5589 0.21 -2.1
6,406,577 - Pf6N2E2_5589 0.21 -1.3
6,406,758 + Pf6N2E2_5589 0.40 -3.1
6,406,759 - Pf6N2E2_5589 0.40 -4.1
6,407,158 + Pf6N2E2_5589 0.81 -3.3
6,407,159 - Pf6N2E2_5589 0.81 -2.7
6,407,159 - Pf6N2E2_5589 0.81 -2.2
6,407,378 - +0.5
6,407,378 - -2.4
6,407,398 + +1.2

Or see this region's nucleotide sequence