Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5347

Experiment: inner cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5346 and Pf6N2E2_5347 are separated by 868 nucleotidesPf6N2E2_5347 and Pf6N2E2_5348 are separated by 119 nucleotidesPf6N2E2_5348 and Pf6N2E2_5349 overlap by 4 nucleotides Pf6N2E2_5346 - rRNA small subunit methyltransferase I, at 6,140,216 to 6,141,088 _5346 Pf6N2E2_5347 - Cell division protein MraZ, at 6,141,957 to 6,142,280 _5347 Pf6N2E2_5348 - rRNA small subunit methyltransferase H, at 6,142,400 to 6,143,224 _5348 Pf6N2E2_5349 - Cell division protein FtsL, at 6,143,221 to 6,143,514 _5349 Position (kb) 6141 6142 6143Strain fitness (log2 ratio) -2 -1 0 1 2at 6140.979 kb on + strand, within Pf6N2E2_5346at 6140.980 kb on - strand, within Pf6N2E2_5346at 6141.120 kb on - strandat 6141.558 kb on - strandat 6141.560 kb on + strandat 6141.561 kb on - strandat 6141.593 kb on + strandat 6141.693 kb on + strandat 6141.693 kb on + strandat 6141.744 kb on + strandat 6141.744 kb on + strandat 6141.744 kb on + strandat 6141.744 kb on + strandat 6141.744 kb on + strandat 6141.746 kb on + strandat 6141.925 kb on + strandat 6142.156 kb on + strand, within Pf6N2E2_5347

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Per-strain Table

Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay
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6,140,979 + Pf6N2E2_5346 0.87 +0.1
6,140,980 - Pf6N2E2_5346 0.88 -1.2
6,141,120 - -0.6
6,141,558 - -2.3
6,141,560 + -0.2
6,141,561 - -1.5
6,141,593 + +0.5
6,141,693 + -2.1
6,141,693 + -1.0
6,141,744 + -1.0
6,141,744 + +0.1
6,141,744 + +0.4
6,141,744 + -1.2
6,141,744 + -2.0
6,141,746 + -1.7
6,141,925 + +2.4
6,142,156 + Pf6N2E2_5347 0.61 -1.1

Or see this region's nucleotide sequence