Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_522 and Pf6N2E2_523 are separated by 77 nucleotides Pf6N2E2_523 and Pf6N2E2_524 are separated by 148 nucleotides
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Pf6N2E2_523 - Inositol transport system ATP-binding protein, at 591,944 to 593,497
_523
Pf6N2E2_524 - Inositol transport system permease protein, at 593,646 to 594,668
_524
Position (kb)
591
592
593
594 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522 at 591.658 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.897 kb on - strand at 591.935 kb on + strand at 591.935 kb on + strand at 591.935 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 591.999 kb on + strand at 592.000 kb on - strand at 592.000 kb on - strand at 592.002 kb on + strand at 592.003 kb on - strand at 592.003 kb on - strand at 592.003 kb on - strand at 592.017 kb on - strand at 592.017 kb on - strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.077 kb on + strand at 592.078 kb on - strand at 592.169 kb on + strand, within Pf6N2E2_523 at 592.169 kb on + strand, within Pf6N2E2_523 at 592.170 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.482 kb on - strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.595 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.664 kb on + strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.665 kb on - strand, within Pf6N2E2_523 at 592.924 kb on + strand, within Pf6N2E2_523 at 592.963 kb on + strand, within Pf6N2E2_523 at 593.087 kb on + strand, within Pf6N2E2_523 at 593.087 kb on + strand, within Pf6N2E2_523 at 593.087 kb on + strand, within Pf6N2E2_523 at 593.087 kb on + strand, within Pf6N2E2_523 at 593.088 kb on - strand, within Pf6N2E2_523 at 593.312 kb on + strand, within Pf6N2E2_523 at 593.312 kb on + strand, within Pf6N2E2_523 at 593.327 kb on + strand, within Pf6N2E2_523 at 593.328 kb on - strand, within Pf6N2E2_523 at 593.328 kb on - strand, within Pf6N2E2_523 at 593.383 kb on - strand at 593.515 kb on + strand at 593.515 kb on + strand at 593.515 kb on + strand at 593.515 kb on + strand at 593.516 kb on - strand at 593.549 kb on - strand at 593.645 kb on + strand at 593.646 kb on - strand at 593.720 kb on + strand at 593.720 kb on + strand at 593.721 kb on - strand at 593.721 kb on - strand at 593.721 kb on - strand at 594.080 kb on + strand, within Pf6N2E2_524 at 594.141 kb on + strand, within Pf6N2E2_524 at 594.141 kb on + strand, within Pf6N2E2_524 at 594.141 kb on + strand, within Pf6N2E2_524 at 594.141 kb on + strand, within Pf6N2E2_524 at 594.141 kb on + strand, within Pf6N2E2_524 at 594.142 kb on - strand, within Pf6N2E2_524 at 594.142 kb on - strand, within Pf6N2E2_524 at 594.180 kb on + strand, within Pf6N2E2_524 at 594.180 kb on + strand, within Pf6N2E2_524 at 594.180 kb on + strand, within Pf6N2E2_524 at 594.181 kb on - strand, within Pf6N2E2_524 at 594.245 kb on + strand, within Pf6N2E2_524 at 594.268 kb on - strand, within Pf6N2E2_524 at 594.276 kb on + strand, within Pf6N2E2_524 at 594.276 kb on + strand, within Pf6N2E2_524 at 594.276 kb on + strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.277 kb on - strand, within Pf6N2E2_524 at 594.282 kb on + strand, within Pf6N2E2_524 at 594.282 kb on + strand, within Pf6N2E2_524 at 594.282 kb on + strand, within Pf6N2E2_524 at 594.283 kb on - strand, within Pf6N2E2_524 at 594.354 kb on + strand, within Pf6N2E2_524 at 594.354 kb on + strand, within Pf6N2E2_524 at 594.355 kb on - strand, within Pf6N2E2_524 at 594.355 kb on - strand, within Pf6N2E2_524 at 594.355 kb on - strand, within Pf6N2E2_524 at 594.441 kb on + strand, within Pf6N2E2_524 at 594.441 kb on + strand, within Pf6N2E2_524 at 594.442 kb on - strand, within Pf6N2E2_524 at 594.442 kb on - strand, within Pf6N2E2_524
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 591,060 + Pf6N2E2_522 0.13 +1.6 591,061 - Pf6N2E2_522 0.13 -1.8 591,061 - Pf6N2E2_522 0.13 +0.1 591,478 - Pf6N2E2_522 0.58 -1.5 591,658 + Pf6N2E2_522 0.77 +2.1 591,770 + Pf6N2E2_522 0.90 -1.3 591,770 + Pf6N2E2_522 0.90 -1.2 591,897 - -0.1 591,935 + +0.8 591,935 + -0.6 591,935 + +0.1 591,999 + +0.6 591,999 + -0.2 591,999 + +0.6 591,999 + +0.1 591,999 + +0.8 591,999 + +1.2 592,000 - -1.3 592,000 - +0.1 592,002 + -0.2 592,003 - +1.8 592,003 - +0.8 592,003 - -0.9 592,017 - +1.8 592,017 - -1.5 592,077 + -1.3 592,077 + +0.6 592,077 + -2.1 592,077 + +0.2 592,077 + +0.4 592,077 + +1.1 592,078 - -0.3 592,169 + Pf6N2E2_523 0.14 -1.7 592,169 + Pf6N2E2_523 0.14 +0.4 592,170 - Pf6N2E2_523 0.15 +1.3 592,482 - Pf6N2E2_523 0.35 -1.4 592,482 - Pf6N2E2_523 0.35 -1.1 592,482 - Pf6N2E2_523 0.35 +0.0 592,482 - Pf6N2E2_523 0.35 +2.6 592,482 - Pf6N2E2_523 0.35 +0.9 592,595 + Pf6N2E2_523 0.42 +0.9 592,595 + Pf6N2E2_523 0.42 +3.3 592,595 + Pf6N2E2_523 0.42 +1.7 592,595 + Pf6N2E2_523 0.42 -0.9 592,595 + Pf6N2E2_523 0.42 -0.5 592,595 + Pf6N2E2_523 0.42 +0.2 592,595 + Pf6N2E2_523 0.42 +1.7 592,595 + Pf6N2E2_523 0.42 +0.3 592,595 + Pf6N2E2_523 0.42 +1.0 592,595 + Pf6N2E2_523 0.42 -2.3 592,664 + Pf6N2E2_523 0.46 -0.5 592,664 + Pf6N2E2_523 0.46 -0.0 592,664 + Pf6N2E2_523 0.46 -0.4 592,664 + Pf6N2E2_523 0.46 -0.2 592,664 + Pf6N2E2_523 0.46 +1.3 592,665 - Pf6N2E2_523 0.46 -0.8 592,665 - Pf6N2E2_523 0.46 -1.3 592,665 - Pf6N2E2_523 0.46 -0.4 592,665 - Pf6N2E2_523 0.46 -3.1 592,665 - Pf6N2E2_523 0.46 +1.5 592,665 - Pf6N2E2_523 0.46 +0.6 592,924 + Pf6N2E2_523 0.63 +0.0 592,963 + Pf6N2E2_523 0.66 -0.5 593,087 + Pf6N2E2_523 0.74 -1.1 593,087 + Pf6N2E2_523 0.74 -4.0 593,087 + Pf6N2E2_523 0.74 +0.9 593,087 + Pf6N2E2_523 0.74 -2.1 593,088 - Pf6N2E2_523 0.74 +0.6 593,312 + Pf6N2E2_523 0.88 -0.7 593,312 + Pf6N2E2_523 0.88 +1.3 593,327 + Pf6N2E2_523 0.89 +0.4 593,328 - Pf6N2E2_523 0.89 -0.7 593,328 - Pf6N2E2_523 0.89 +2.7 593,383 - +0.4 593,515 + +0.9 593,515 + +0.1 593,515 + -0.9 593,515 + -0.6 593,516 - +0.8 593,549 - -0.3 593,645 + -0.1 593,646 - +0.0 593,720 + -0.9 593,720 + +3.4 593,721 - -0.9 593,721 - +1.3 593,721 - -1.0 594,080 + Pf6N2E2_524 0.42 -0.4 594,141 + Pf6N2E2_524 0.48 +0.6 594,141 + Pf6N2E2_524 0.48 +0.1 594,141 + Pf6N2E2_524 0.48 +1.3 594,141 + Pf6N2E2_524 0.48 -0.2 594,141 + Pf6N2E2_524 0.48 -1.0 594,142 - Pf6N2E2_524 0.48 -0.2 594,142 - Pf6N2E2_524 0.48 -1.2 594,180 + Pf6N2E2_524 0.52 +0.8 594,180 + Pf6N2E2_524 0.52 +1.1 594,180 + Pf6N2E2_524 0.52 +1.3 594,181 - Pf6N2E2_524 0.52 -0.3 594,245 + Pf6N2E2_524 0.59 -1.0 594,268 - Pf6N2E2_524 0.61 +1.0 594,276 + Pf6N2E2_524 0.62 -0.8 594,276 + Pf6N2E2_524 0.62 -0.1 594,276 + Pf6N2E2_524 0.62 +0.6 594,277 - Pf6N2E2_524 0.62 +0.5 594,277 - Pf6N2E2_524 0.62 +0.8 594,277 - Pf6N2E2_524 0.62 -0.6 594,277 - Pf6N2E2_524 0.62 -0.5 594,277 - Pf6N2E2_524 0.62 +1.2 594,277 - Pf6N2E2_524 0.62 -2.6 594,282 + Pf6N2E2_524 0.62 +1.2 594,282 + Pf6N2E2_524 0.62 -0.2 594,282 + Pf6N2E2_524 0.62 +0.1 594,283 - Pf6N2E2_524 0.62 -1.1 594,354 + Pf6N2E2_524 0.69 -0.0 594,354 + Pf6N2E2_524 0.69 -0.0 594,355 - Pf6N2E2_524 0.69 +0.7 594,355 - Pf6N2E2_524 0.69 -1.1 594,355 - Pf6N2E2_524 0.69 +0.1 594,441 + Pf6N2E2_524 0.78 +0.4 594,441 + Pf6N2E2_524 0.78 -2.5 594,442 - Pf6N2E2_524 0.78 +0.0 594,442 - Pf6N2E2_524 0.78 +1.8
Or see this region's nucleotide sequence