Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5184

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
5,977,979	-		Pf6N2E2_5183	0.65	-0.6
5,977,979	-		Pf6N2E2_5183	0.65	-1.4
5,978,077	+		Pf6N2E2_5183	0.70	+0.6
5,978,077	+		Pf6N2E2_5183	0.70	-1.0
5,978,078	-		Pf6N2E2_5183	0.70	-2.8
5,978,168	-		Pf6N2E2_5183	0.74	+0.0
5,978,280	-		Pf6N2E2_5183	0.79	-0.4
5,978,280	-		Pf6N2E2_5183	0.79	+0.6
5,978,280	-		Pf6N2E2_5183	0.79	-0.3
5,978,435	+		Pf6N2E2_5183	0.85	+0.2
5,978,435	+		Pf6N2E2_5183	0.85	-0.4
5,978,436	-		Pf6N2E2_5183	0.85	-0.7
5,978,446	+		Pf6N2E2_5183	0.86	+0.5
5,978,447	-		Pf6N2E2_5183	0.86	+0.2
5,978,526	-		Pf6N2E2_5183	0.89	+1.1
5,978,526	-		Pf6N2E2_5183	0.89	-1.4
5,978,563	+				-0.3
5,978,564	-				+0.5
5,978,564	-				-0.4
5,978,722	+				+1.4
5,978,774	+				-2.5
5,978,774	+				+0.7
5,978,774	+				-1.2
5,978,774	+				+0.4
5,978,774	+				-1.9
5,978,774	+				-0.5
5,978,774	+				-0.1
5,978,774	+				-0.2
5,978,774	+				-0.8
5,978,774	+				-0.5
5,978,774	+				+0.2
5,978,775	-				-2.3
5,978,775	-				+1.2
5,978,775	-				-0.3
5,978,775	-				+0.3
5,978,780	+				-0.4
5,978,780	+				+0.8
5,978,781	-				+0.4
5,978,781	-				-1.6
5,978,781	-				+0.0
5,978,781	-				+0.5
5,978,781	-				-1.1
5,978,790	+				-0.8
5,978,839	+				+0.5
5,978,893	+				+1.9
5,978,893	+				-3.0
5,978,893	+				-2.6
5,978,893	+				-1.3
5,978,893	+				+0.6
5,978,893	+				+0.3
5,978,894	-				+0.7
5,978,894	-				-1.5
5,978,894	-				+0.4
5,978,894	-				+0.1
5,978,897	+				-1.6
5,978,897	+				-1.4
5,978,897	+				-0.8
5,978,897	+				-0.9
5,978,897	+				-0.2
5,978,897	+				+0.4
5,978,897	+				-0.8
5,978,897	+				+2.1
5,978,897	+				+0.7
5,978,897	+				-0.3
5,978,897	+				+0.2
5,978,897	+				-0.0
5,978,897	+				+0.2
5,978,897	+				-3.2
5,978,897	+				+0.5
5,978,898	-				-0.5
5,978,898	-				+1.4
5,978,898	-				+0.0
5,978,898	-				+0.7
5,978,898	-				-2.1
5,978,898	-				+0.5
5,978,898	-				-0.8
5,978,898	-				-0.1
5,978,898	-				+0.3
5,978,900	+				-1.7
5,978,901	-				-0.1
5,978,926	+				+1.3
5,978,926	+				+0.8
5,978,927	-				-0.7
5,978,927	-				-0.4
5,979,033	+				-1.2
5,979,034	-				-2.4
5,979,034	-				-2.6
5,979,034	-				-2.2
5,979,034	-				-1.2
5,979,034	-				-2.2
5,979,053	+				-2.7
5,979,053	+				-3.3
5,979,053	+				-1.6
5,979,054	-				-0.7
5,979,054	-				-1.2
5,979,054	-				-1.5
5,979,054	-				-0.8
5,979,054	-				-0.6
5,979,054	-				-3.0
5,979,054	-				+0.4
5,979,057	+				-2.9
5,979,057	+				-2.9
5,979,057	+				-2.8
5,979,057	+				-2.9
5,979,057	+				+0.3
5,979,057	+				-2.2
5,979,057	+				-1.3
5,979,057	+				-2.3
5,979,058	-				-3.2
5,979,058	-				-0.8
5,979,058	-				-0.5
5,979,058	-				-1.5
5,979,216	+				-3.2
5,979,217	-				-2.2
5,979,217	-				+0.1
5,979,217	-				-0.2
5,979,219	+				-0.7
5,979,220	-				-0.6
5,979,273	+		Pf6N2E2_5184	0.11	-1.5
5,979,273	+		Pf6N2E2_5184	0.11	+0.9
5,979,273	+		Pf6N2E2_5184	0.11	-1.9
5,979,273	+		Pf6N2E2_5184	0.11	-2.2
5,979,273	+		Pf6N2E2_5184	0.11	-2.8
5,979,273	+		Pf6N2E2_5184	0.11	-1.2
5,979,273	+		Pf6N2E2_5184	0.11	-3.2
5,979,273	+		Pf6N2E2_5184	0.11	-0.7
5,979,273	+		Pf6N2E2_5184	0.11	-2.2
5,979,273	+		Pf6N2E2_5184	0.11	-3.8
5,979,273	+		Pf6N2E2_5184	0.11	-1.8
5,979,273	+		Pf6N2E2_5184	0.11	-1.3
5,979,273	+		Pf6N2E2_5184	0.11	-3.3
5,979,274	-		Pf6N2E2_5184	0.11	-0.7
5,979,274	-		Pf6N2E2_5184	0.11	-2.0
5,979,274	-		Pf6N2E2_5184	0.11	+0.3
5,979,274	-		Pf6N2E2_5184	0.11	-0.1
5,979,274	-		Pf6N2E2_5184	0.11	-0.9
5,979,274	-		Pf6N2E2_5184	0.11	-2.2
5,979,274	-		Pf6N2E2_5184	0.11	-1.9
5,979,274	-		Pf6N2E2_5184	0.11	-3.0
5,979,442	-		Pf6N2E2_5184	0.17	-0.8
5,979,523	-		Pf6N2E2_5184	0.20	-1.2
5,979,670	-		Pf6N2E2_5184	0.25	-3.4
5,979,766	-		Pf6N2E2_5184	0.28	+0.3
5,979,850	-		Pf6N2E2_5184	0.31	-2.4
5,979,850	-		Pf6N2E2_5184	0.31	-2.1
5,979,850	-		Pf6N2E2_5184	0.31	-3.4
5,979,850	-		Pf6N2E2_5184	0.31	-1.3
5,979,903	+		Pf6N2E2_5184	0.33	-1.1
5,979,903	+		Pf6N2E2_5184	0.33	-2.3
5,979,903	+		Pf6N2E2_5184	0.33	-2.7
5,979,903	+		Pf6N2E2_5184	0.33	-2.0
5,980,242	+		Pf6N2E2_5184	0.45	-2.7
5,980,596	+		Pf6N2E2_5184	0.58	-3.8
5,980,596	+		Pf6N2E2_5184	0.58	-0.6
5,980,597	-		Pf6N2E2_5184	0.58	+0.3
5,980,597	-		Pf6N2E2_5184	0.58	-2.6
5,980,597	-		Pf6N2E2_5184	0.58	-2.6
5,980,960	-		Pf6N2E2_5184	0.70	-1.4
5,981,091	+		Pf6N2E2_5184	0.75	-1.8
5,981,091	+		Pf6N2E2_5184	0.75	-2.8
5,981,091	+		Pf6N2E2_5184	0.75	-1.6
5,981,091	+		Pf6N2E2_5184	0.75	-0.5
5,981,091	+		Pf6N2E2_5184	0.75	-0.0
5,981,092	-		Pf6N2E2_5184	0.75	-2.7
5,981,092	-		Pf6N2E2_5184	0.75	-1.9
5,981,092	-		Pf6N2E2_5184	0.75	-1.6
5,981,092	-		Pf6N2E2_5184	0.75	-1.3
5,981,145	+		Pf6N2E2_5184	0.77	-3.1
5,981,145	+		Pf6N2E2_5184	0.77	-0.6
5,981,146	-		Pf6N2E2_5184	0.77	-2.2
5,981,146	-		Pf6N2E2_5184	0.77	+1.2
5,981,146	-		Pf6N2E2_5184	0.77	-1.7
5,981,146	-		Pf6N2E2_5184	0.77	-1.1
5,981,371	-		Pf6N2E2_5184	0.85	-0.7
5,981,565	+				-1.2
5,981,565	+				+0.5
5,981,565	+				+0.4
5,981,565	+				+0.7
5,981,566	-				-2.4
5,981,566	-				-1.1
5,981,566	-				-1.9
5,981,566	-				-1.4
5,981,566	-				-2.9
5,981,566	-				-0.4
5,981,566	-				-1.7
5,981,586	+				-2.2
5,981,586	+				-1.1
5,981,587	-				+2.6
5,981,587	-				-1.4
5,981,587	-				-1.9
5,981,587	-				-2.2
5,981,613	+				-2.4
5,981,613	+				-1.4
5,981,613	+				-1.6
5,981,683	+				-1.7
5,981,703	+				-1.2
5,981,704	-				-0.5
5,981,896	+		Pf6N2E2_5185	0.14	-1.6
5,981,897	-		Pf6N2E2_5185	0.14	-0.3
5,981,897	-		Pf6N2E2_5185	0.14	+0.6
5,982,038	+		Pf6N2E2_5185	0.34	-1.3
5,982,038	+		Pf6N2E2_5185	0.34	-3.1
5,982,039	-		Pf6N2E2_5185	0.34	-3.4
5,982,118	+		Pf6N2E2_5185	0.46	-2.1
5,982,314	+		Pf6N2E2_5185	0.74	-1.8
5,982,314	+		Pf6N2E2_5185	0.74	-0.2
5,982,314	+		Pf6N2E2_5185	0.74	-1.3
5,982,314	+		Pf6N2E2_5185	0.74	-2.1
5,982,314	+		Pf6N2E2_5185	0.74	-1.6
5,982,315	-		Pf6N2E2_5185	0.74	-2.9
5,982,315	-		Pf6N2E2_5185	0.74	-2.8
5,982,315	-		Pf6N2E2_5185	0.74	-2.0
5,982,317	+		Pf6N2E2_5185	0.74	+0.2
5,982,317	+		Pf6N2E2_5185	0.74	+0.1
5,982,317	+		Pf6N2E2_5185	0.74	-1.4
5,982,317	+		Pf6N2E2_5185	0.74	-0.8
5,982,318	-		Pf6N2E2_5185	0.74	-0.2
5,982,375	-		Pf6N2E2_5185	0.82	-3.1
5,982,405	-		Pf6N2E2_5185	0.87	-0.4
5,982,434	+				-1.9
5,982,484	+				+1.5
5,982,485	-				-2.1
5,982,588	+				-2.5
5,982,588	+				+0.7
5,982,588	+				-3.3