Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5127 and Pf6N2E2_5128 overlap by 4 nucleotides Pf6N2E2_5128 and Pf6N2E2_5129 are separated by 130 nucleotides Pf6N2E2_5129 and Pf6N2E2_6146 are separated by 415 nucleotides Pf6N2E2_6146 and Pf6N2E2_6147 are separated by 10 nucleotides Pf6N2E2_6147 and Pf6N2E2_6148 are separated by 8 nucleotides Pf6N2E2_6148 and Pf6N2E2_6149 are separated by 96 nucleotides Pf6N2E2_6149 and Pf6N2E2_5130 overlap by 9 nucleotides Pf6N2E2_5130 and Pf6N2E2_5131 are separated by 30 nucleotides
Pf6N2E2_5127 - Ribosomal large subunit pseudouridine synthase D (EC 4.2.1.70), at 5,911,362 to 5,912,282
_5127
Pf6N2E2_5128 - COG1496: Uncharacterized conserved protein, at 5,912,279 to 5,913,004
_5128
Pf6N2E2_5129 - ClpB protein, at 5,913,135 to 5,915,699
_5129
Pf6N2E2_6146 - tRNA-Asn-GTT, at 5,916,115 to 5,916,187
_6146
Pf6N2E2_6147 - tRNA-Pro-TGG, at 5,916,198 to 5,916,271
_6147
Pf6N2E2_6148 - tRNA-Lys-TTT, at 5,916,280 to 5,916,352
_6148
Pf6N2E2_6149 - tRNA-Pro-TGG, at 5,916,449 to 5,916,522
_6149
Pf6N2E2_5130 - hypothetical protein, at 5,916,514 to 5,916,642
_5130
Pf6N2E2_5131 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 5,916,673 to 5,918,298
_5131
Position (kb)
5913
5914
5915
5916 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5912.472 kb on + strand, within Pf6N2E2_5128 at 5912.851 kb on + strand, within Pf6N2E2_5128 at 5912.855 kb on + strand, within Pf6N2E2_5128 at 5912.855 kb on + strand, within Pf6N2E2_5128 at 5912.856 kb on - strand, within Pf6N2E2_5128 at 5912.856 kb on - strand, within Pf6N2E2_5128 at 5912.903 kb on + strand, within Pf6N2E2_5128 at 5913.003 kb on - strand at 5913.019 kb on + strand at 5913.020 kb on - strand at 5913.020 kb on - strand at 5913.062 kb on + strand at 5913.062 kb on + strand at 5913.062 kb on + strand at 5913.062 kb on + strand at 5913.062 kb on + strand at 5913.062 kb on + strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.063 kb on - strand at 5913.070 kb on + strand at 5913.071 kb on - strand at 5913.071 kb on - strand at 5913.071 kb on - strand at 5913.071 kb on - strand at 5913.121 kb on + strand at 5913.140 kb on + strand at 5913.140 kb on + strand at 5913.140 kb on + strand at 5913.140 kb on + strand at 5913.141 kb on - strand at 5913.141 kb on - strand at 5913.163 kb on + strand at 5913.163 kb on + strand at 5913.164 kb on - strand at 5913.164 kb on - strand at 5913.164 kb on - strand at 5913.164 kb on - strand at 5913.347 kb on - strand at 5913.552 kb on - strand, within Pf6N2E2_5129 at 5913.624 kb on + strand, within Pf6N2E2_5129 at 5913.624 kb on + strand, within Pf6N2E2_5129 at 5913.624 kb on + strand, within Pf6N2E2_5129 at 5913.624 kb on + strand, within Pf6N2E2_5129 at 5913.624 kb on + strand, within Pf6N2E2_5129 at 5913.625 kb on - strand at 5913.625 kb on - strand, within Pf6N2E2_5129 at 5913.625 kb on - strand, within Pf6N2E2_5129 at 5913.625 kb on - strand, within Pf6N2E2_5129 at 5913.625 kb on - strand, within Pf6N2E2_5129 at 5913.701 kb on + strand, within Pf6N2E2_5129 at 5913.702 kb on - strand, within Pf6N2E2_5129 at 5913.767 kb on + strand, within Pf6N2E2_5129 at 5913.768 kb on - strand, within Pf6N2E2_5129 at 5913.836 kb on + strand, within Pf6N2E2_5129 at 5913.885 kb on + strand, within Pf6N2E2_5129 at 5913.885 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.098 kb on + strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.099 kb on - strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.100 kb on + strand, within Pf6N2E2_5129 at 5914.101 kb on - strand, within Pf6N2E2_5129 at 5914.101 kb on - strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.102 kb on + strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.103 kb on - strand, within Pf6N2E2_5129 at 5914.203 kb on + strand, within Pf6N2E2_5129 at 5914.204 kb on - strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.272 kb on + strand, within Pf6N2E2_5129 at 5914.273 kb on - strand, within Pf6N2E2_5129 at 5914.273 kb on - strand, within Pf6N2E2_5129 at 5914.607 kb on + strand, within Pf6N2E2_5129 at 5914.641 kb on + strand, within Pf6N2E2_5129 at 5914.642 kb on - strand, within Pf6N2E2_5129 at 5915.091 kb on + strand, within Pf6N2E2_5129 at 5915.100 kb on + strand, within Pf6N2E2_5129 at 5915.101 kb on - strand, within Pf6N2E2_5129 at 5915.115 kb on + strand, within Pf6N2E2_5129 at 5915.116 kb on - strand, within Pf6N2E2_5129 at 5915.563 kb on - strand at 5915.936 kb on + strand at 5915.936 kb on + strand at 5916.387 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.526 kb on + strand at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.541 kb on + strand, within Pf6N2E2_5130 at 5916.551 kb on + strand, within Pf6N2E2_5130 at 5916.657 kb on - strand at 5916.672 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,912,472 + Pf6N2E2_5128 0.27 -3.7 5,912,851 + Pf6N2E2_5128 0.79 -2.7 5,912,855 + Pf6N2E2_5128 0.79 -1.2 5,912,855 + Pf6N2E2_5128 0.79 -0.8 5,912,856 - Pf6N2E2_5128 0.79 -0.6 5,912,856 - Pf6N2E2_5128 0.79 +0.8 5,912,903 + Pf6N2E2_5128 0.86 -2.7 5,913,003 - +0.8 5,913,019 + -0.4 5,913,020 - +0.3 5,913,020 - +1.7 5,913,062 + -0.4 5,913,062 + +1.2 5,913,062 + -0.0 5,913,062 + -0.0 5,913,062 + +0.4 5,913,062 + +0.7 5,913,063 - -3.3 5,913,063 - -2.9 5,913,063 - -1.5 5,913,063 - -3.6 5,913,063 - +0.2 5,913,063 - -1.9 5,913,063 - -3.1 5,913,063 - -1.0 5,913,063 - -2.9 5,913,063 - -1.8 5,913,070 + -0.3 5,913,071 - -2.2 5,913,071 - -1.6 5,913,071 - -1.5 5,913,071 - -2.8 5,913,121 + -1.1 5,913,140 + -1.1 5,913,140 + -1.4 5,913,140 + -2.4 5,913,140 + -3.0 5,913,141 - -1.5 5,913,141 - -1.9 5,913,163 + -0.3 5,913,163 + -1.2 5,913,164 - -2.1 5,913,164 - -0.7 5,913,164 - -0.5 5,913,164 - -0.0 5,913,347 - -1.6 5,913,552 - Pf6N2E2_5129 0.16 -2.3 5,913,624 + Pf6N2E2_5129 0.19 -2.8 5,913,624 + Pf6N2E2_5129 0.19 -2.5 5,913,624 + Pf6N2E2_5129 0.19 -2.6 5,913,624 + Pf6N2E2_5129 0.19 -1.9 5,913,624 + Pf6N2E2_5129 0.19 -2.6 5,913,625 - +1.3 5,913,625 - Pf6N2E2_5129 0.19 -2.5 5,913,625 - Pf6N2E2_5129 0.19 -1.7 5,913,625 - Pf6N2E2_5129 0.19 -0.5 5,913,625 - Pf6N2E2_5129 0.19 -3.3 5,913,701 + Pf6N2E2_5129 0.22 -0.5 5,913,702 - Pf6N2E2_5129 0.22 -1.9 5,913,767 + Pf6N2E2_5129 0.25 -2.1 5,913,768 - Pf6N2E2_5129 0.25 -1.7 5,913,836 + Pf6N2E2_5129 0.27 -2.8 5,913,885 + Pf6N2E2_5129 0.29 -2.9 5,913,885 + Pf6N2E2_5129 0.29 -1.6 5,914,098 + Pf6N2E2_5129 0.38 -1.9 5,914,098 + Pf6N2E2_5129 0.38 +0.7 5,914,098 + Pf6N2E2_5129 0.38 -1.5 5,914,098 + Pf6N2E2_5129 0.38 -2.1 5,914,098 + Pf6N2E2_5129 0.38 -2.0 5,914,098 + Pf6N2E2_5129 0.38 -1.5 5,914,098 + Pf6N2E2_5129 0.38 -0.8 5,914,098 + Pf6N2E2_5129 0.38 -3.3 5,914,098 + Pf6N2E2_5129 0.38 -2.0 5,914,098 + Pf6N2E2_5129 0.38 -1.8 5,914,099 - Pf6N2E2_5129 0.38 -0.1 5,914,099 - Pf6N2E2_5129 0.38 -2.1 5,914,099 - Pf6N2E2_5129 0.38 -2.7 5,914,099 - Pf6N2E2_5129 0.38 -3.2 5,914,099 - Pf6N2E2_5129 0.38 -2.0 5,914,099 - Pf6N2E2_5129 0.38 -1.2 5,914,099 - Pf6N2E2_5129 0.38 -3.3 5,914,099 - Pf6N2E2_5129 0.38 -2.8 5,914,099 - Pf6N2E2_5129 0.38 -2.3 5,914,100 + Pf6N2E2_5129 0.38 -1.3 5,914,100 + Pf6N2E2_5129 0.38 -1.4 5,914,100 + Pf6N2E2_5129 0.38 -1.7 5,914,100 + Pf6N2E2_5129 0.38 -2.9 5,914,100 + Pf6N2E2_5129 0.38 -3.5 5,914,100 + Pf6N2E2_5129 0.38 -0.5 5,914,100 + Pf6N2E2_5129 0.38 -1.8 5,914,100 + Pf6N2E2_5129 0.38 -3.2 5,914,100 + Pf6N2E2_5129 0.38 -2.7 5,914,100 + Pf6N2E2_5129 0.38 -2.1 5,914,101 - Pf6N2E2_5129 0.38 -3.4 5,914,101 - Pf6N2E2_5129 0.38 -1.9 5,914,102 + Pf6N2E2_5129 0.38 -0.7 5,914,102 + Pf6N2E2_5129 0.38 -2.4 5,914,102 + Pf6N2E2_5129 0.38 -3.8 5,914,102 + Pf6N2E2_5129 0.38 -1.9 5,914,102 + Pf6N2E2_5129 0.38 -2.2 5,914,102 + Pf6N2E2_5129 0.38 -1.6 5,914,102 + Pf6N2E2_5129 0.38 -3.7 5,914,102 + Pf6N2E2_5129 0.38 -3.1 5,914,102 + Pf6N2E2_5129 0.38 -1.5 5,914,102 + Pf6N2E2_5129 0.38 -2.6 5,914,103 - Pf6N2E2_5129 0.38 -2.7 5,914,103 - Pf6N2E2_5129 0.38 -0.9 5,914,103 - Pf6N2E2_5129 0.38 -2.7 5,914,103 - Pf6N2E2_5129 0.38 -2.6 5,914,103 - Pf6N2E2_5129 0.38 -1.9 5,914,103 - Pf6N2E2_5129 0.38 -1.6 5,914,103 - Pf6N2E2_5129 0.38 -2.9 5,914,103 - Pf6N2E2_5129 0.38 -1.7 5,914,203 + Pf6N2E2_5129 0.42 -2.6 5,914,204 - Pf6N2E2_5129 0.42 -2.5 5,914,272 + Pf6N2E2_5129 0.44 -1.7 5,914,272 + Pf6N2E2_5129 0.44 -1.7 5,914,272 + Pf6N2E2_5129 0.44 -0.5 5,914,272 + Pf6N2E2_5129 0.44 -2.9 5,914,272 + Pf6N2E2_5129 0.44 -3.9 5,914,272 + Pf6N2E2_5129 0.44 -2.3 5,914,273 - Pf6N2E2_5129 0.44 -2.4 5,914,273 - Pf6N2E2_5129 0.44 -2.5 5,914,607 + Pf6N2E2_5129 0.57 -1.9 5,914,641 + Pf6N2E2_5129 0.59 -2.9 5,914,642 - Pf6N2E2_5129 0.59 -2.8 5,915,091 + Pf6N2E2_5129 0.76 -2.6 5,915,100 + Pf6N2E2_5129 0.77 -1.6 5,915,101 - Pf6N2E2_5129 0.77 -1.2 5,915,115 + Pf6N2E2_5129 0.77 -2.7 5,915,116 - Pf6N2E2_5129 0.77 -1.7 5,915,563 - -1.7 5,915,936 + -0.1 5,915,936 + +1.0 5,916,387 + -0.3 5,916,526 + +0.5 5,916,526 + -2.1 5,916,526 + -0.9 5,916,541 + Pf6N2E2_5130 0.21 +0.6 5,916,541 + Pf6N2E2_5130 0.21 -2.8 5,916,551 + Pf6N2E2_5130 0.29 +0.3 5,916,657 - -1.1 5,916,672 + +1.7
Or see this region's nucleotide sequence