Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5013 and Pf6N2E2_5014 are separated by 168 nucleotides Pf6N2E2_5014 and Pf6N2E2_5015 are separated by 27 nucleotides Pf6N2E2_5015 and Pf6N2E2_5016 are separated by 40 nucleotides
Pf6N2E2_5013 - Putative membrane protein, at 5,779,651 to 5,780,412
_5013
Pf6N2E2_5014 - Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE, at 5,780,581 to 5,782,104
_5014
Pf6N2E2_5015 - Magnesium and cobalt efflux protein CorC, at 5,782,132 to 5,782,971
_5015
Pf6N2E2_5016 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 5,783,012 to 5,783,470
_5016
Position (kb) 5780
5781
5782
5783 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5779.623 kb on - strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.664 kb on - strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.956 kb on + strand, within Pf6N2E2_5013 at 5779.956 kb on + strand, within Pf6N2E2_5013 at 5780.044 kb on + strand, within Pf6N2E2_5013 at 5780.130 kb on + strand, within Pf6N2E2_5013 at 5780.404 kb on + strand at 5780.405 kb on - strand at 5780.434 kb on + strand at 5780.453 kb on + strand at 5780.548 kb on + strand at 5780.549 kb on - strand at 5780.549 kb on - strand at 5781.087 kb on - strand, within Pf6N2E2_5014 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5782.380 kb on - strand, within Pf6N2E2_5015 at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,779,623 - -0.1 5,779,637 + +1.6 5,779,637 + +1.1 5,779,637 + +1.7 5,779,637 + -2.1 5,779,637 + -0.1 5,779,637 + +1.6 5,779,637 + +0.6 5,779,637 + +1.4 5,779,637 + +1.0 5,779,637 + +0.8 5,779,637 + -0.9 5,779,637 + +0.6 5,779,638 - -3.6 5,779,638 - -0.2 5,779,638 - +1.3 5,779,638 - +0.4 5,779,638 - -0.8 5,779,638 - -0.0 5,779,638 - +1.6 5,779,638 - -0.6 5,779,664 - -0.1 5,779,682 + -1.4 5,779,682 + +0.9 5,779,682 + +0.2 5,779,682 + -0.2 5,779,683 - -0.2 5,779,683 - +1.9 5,779,683 - -0.7 5,779,683 - +0.5 5,779,956 + Pf6N2E2_5013 0.40 -1.4 5,779,956 + Pf6N2E2_5013 0.40 +0.0 5,780,044 + Pf6N2E2_5013 0.52 +0.2 5,780,130 + Pf6N2E2_5013 0.63 -0.7 5,780,404 + +1.0 5,780,405 - +0.4 5,780,434 + +2.2 5,780,453 + -0.7 5,780,548 + -1.1 5,780,549 - -1.0 5,780,549 - -2.3 5,781,087 - Pf6N2E2_5014 0.33 -2.6 5,782,380 - Pf6N2E2_5015 0.30 -1.1 5,782,380 - Pf6N2E2_5015 0.30 +0.8 5,782,380 - Pf6N2E2_5015 0.30 -1.3 5,782,380 - Pf6N2E2_5015 0.30 +0.4 5,782,380 - Pf6N2E2_5015 0.30 +0.4 5,782,380 - Pf6N2E2_5015 0.30 +0.6 5,782,380 - Pf6N2E2_5015 0.30 +1.4 5,782,380 - Pf6N2E2_5015 0.30 -0.7 5,782,380 - Pf6N2E2_5015 0.30 -0.1 5,782,380 - Pf6N2E2_5015 0.30 +1.0 5,783,011 - -0.4 5,783,011 - +1.3 5,783,011 - -2.6 5,783,011 - -0.4 5,783,011 - +0.1 5,783,011 - +1.0 5,783,011 - -0.1 5,783,011 - +1.0
Or see this region's nucleotide sequence