Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4810

Experiment: inner cut, LB soft agar motility assay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	inner cut, LB soft agar motility assay
5,560,769	-		Pf6N2E2_4809	0.14	-1.6
5,560,851	+		Pf6N2E2_4809	0.22	-1.5
5,560,851	+		Pf6N2E2_4809	0.22	-4.0
5,560,881	+		Pf6N2E2_4809	0.25	+0.3
5,560,954	+		Pf6N2E2_4809	0.32	-2.0
5,560,954	+		Pf6N2E2_4809	0.32	-1.3
5,560,964	+		Pf6N2E2_4809	0.33	-0.7
5,560,964	+		Pf6N2E2_4809	0.33	-1.6
5,560,964	+		Pf6N2E2_4809	0.33	-1.7
5,560,964	+		Pf6N2E2_4809	0.33	-1.7
5,560,965	-		Pf6N2E2_4809	0.33	-2.1
5,560,991	+		Pf6N2E2_4809	0.35	-2.2
5,560,991	+				+2.1
5,560,991	+		Pf6N2E2_4809	0.35	-0.1
5,560,992	-		Pf6N2E2_4809	0.36	-1.3
5,561,059	+		Pf6N2E2_4809	0.42	+0.6
5,561,059	+		Pf6N2E2_4809	0.42	-2.0
5,561,059	+		Pf6N2E2_4809	0.42	-1.8
5,561,059	+		Pf6N2E2_4809	0.42	-1.6
5,561,059	+		Pf6N2E2_4809	0.42	-0.7
5,561,059	+		Pf6N2E2_4809	0.42	-2.0
5,561,059	+		Pf6N2E2_4809	0.42	-1.6
5,561,059	+		Pf6N2E2_4809	0.42	-0.7
5,561,059	+		Pf6N2E2_4809	0.42	-0.8
5,561,059	+		Pf6N2E2_4809	0.42	-3.0
5,561,060	-		Pf6N2E2_4809	0.42	-2.6
5,561,060	-		Pf6N2E2_4809	0.42	-1.5
5,561,060	-		Pf6N2E2_4809	0.42	+0.5
5,561,060	-		Pf6N2E2_4809	0.42	-2.5
5,561,060	-		Pf6N2E2_4809	0.42	+0.6
5,561,060	-		Pf6N2E2_4809	0.42	-2.4
5,561,060	-		Pf6N2E2_4809	0.42	-1.5
5,561,061	-		Pf6N2E2_4809	0.42	+1.8
5,561,107	+		Pf6N2E2_4809	0.47	-0.7
5,561,107	+		Pf6N2E2_4809	0.47	-2.2
5,561,107	+		Pf6N2E2_4809	0.47	-2.2
5,561,107	+		Pf6N2E2_4809	0.47	-1.5
5,561,107	+		Pf6N2E2_4809	0.47	-3.5
5,561,107	+		Pf6N2E2_4809	0.47	-3.1
5,561,108	-		Pf6N2E2_4809	0.47	-3.2
5,561,227	+		Pf6N2E2_4809	0.58	-1.3
5,561,227	+		Pf6N2E2_4809	0.58	-1.2
5,561,227	+		Pf6N2E2_4809	0.58	-1.6
5,561,227	+		Pf6N2E2_4809	0.58	+0.2
5,561,227	+		Pf6N2E2_4809	0.58	-0.9
5,561,227	+		Pf6N2E2_4809	0.58	-1.7
5,561,227	+		Pf6N2E2_4809	0.58	-3.0
5,561,227	+		Pf6N2E2_4809	0.58	-3.6
5,561,227	+		Pf6N2E2_4809	0.58	-1.4
5,561,228	+		Pf6N2E2_4809	0.59	-1.6
5,561,228	-		Pf6N2E2_4809	0.59	-2.1
5,561,228	-		Pf6N2E2_4809	0.59	-1.5
5,561,228	-		Pf6N2E2_4809	0.59	-3.4
5,561,228	-		Pf6N2E2_4809	0.59	-1.5
5,561,228	-		Pf6N2E2_4809	0.59	-0.6
5,561,228	-		Pf6N2E2_4809	0.59	-0.2
5,561,228	-		Pf6N2E2_4809	0.59	-2.8
5,561,228	-		Pf6N2E2_4809	0.59	-2.5
5,561,228	-		Pf6N2E2_4809	0.59	-1.0
5,561,293	+		Pf6N2E2_4809	0.65	-3.2
5,561,294	-		Pf6N2E2_4809	0.65	-2.5
5,561,294	-		Pf6N2E2_4809	0.65	-0.6
5,561,294	-		Pf6N2E2_4809	0.65	-0.7
5,561,294	-		Pf6N2E2_4809	0.65	-2.4
5,561,308	+		Pf6N2E2_4809	0.66	-2.5
5,561,308	+		Pf6N2E2_4809	0.66	-1.4
5,561,308	+		Pf6N2E2_4809	0.66	-1.2
5,561,309	-		Pf6N2E2_4809	0.66	-2.6
5,561,309	-		Pf6N2E2_4809	0.66	-2.3
5,561,383	+		Pf6N2E2_4809	0.74	-2.3
5,561,384	-		Pf6N2E2_4809	0.74	-1.3
5,561,477	+		Pf6N2E2_4809	0.83	-1.0
5,561,477	+		Pf6N2E2_4809	0.83	-1.5
5,561,478	-		Pf6N2E2_4809	0.83	-2.4
5,561,478	-		Pf6N2E2_4809	0.83	-2.1
5,561,478	-		Pf6N2E2_4809	0.83	-2.4
5,561,478	-		Pf6N2E2_4809	0.83	-2.4
5,561,478	-		Pf6N2E2_4809	0.83	-1.4
5,561,478	-		Pf6N2E2_4809	0.83	-1.6
5,561,530	+		Pf6N2E2_4809	0.88	-0.9
5,561,531	-		Pf6N2E2_4809	0.88	-2.7
5,561,560	+				-0.6
5,561,560	+				-0.0
5,561,560	+				-1.5
5,561,560	+				-2.1
5,561,560	+				-2.8
5,561,560	+				-3.2
5,561,560	+				+0.1
5,561,560	+				-1.2
5,561,560	+				-2.1
5,561,560	+				-1.7
5,561,560	+				-0.9
5,561,560	+				-2.4
5,561,560	+				-0.4
5,561,561	-				-3.2
5,561,561	-				-2.2
5,561,561	-				-0.7
5,561,561	-				-2.8
5,561,561	-				-1.0
5,561,561	-				-3.5
5,561,561	-				-0.6
5,561,561	-				-3.0
5,561,561	-				-1.0
5,561,562	+				-0.9
5,561,562	+				-1.6
5,561,562	+				-1.3
5,561,562	+				-1.6
5,561,562	+				-1.3
5,561,562	+				-2.7
5,561,562	+				-1.4
5,561,562	+				-2.0
5,561,562	+				-0.7
5,561,563	-				-3.6
5,561,563	-				-2.4
5,561,564	+				-3.0
5,561,564	+				-0.6
5,561,564	+				-0.4
5,561,564	+				-0.7
5,561,564	+				-2.4
5,561,564	+				-1.5
5,561,564	+				-1.0
5,561,564	+				-1.8
5,561,564	+				-1.0
5,561,564	+				-3.4
5,561,564	+				-0.2
5,561,564	+				+0.4
5,561,564	+				+0.4
5,561,564	+				-2.0
5,561,564	+				-1.7
5,561,565	-				-0.4
5,561,565	-				-0.9
5,561,565	-				-0.8
5,561,565	-				-1.6
5,561,565	-				-1.7
5,561,565	-				-2.0
5,561,565	-				-2.8
5,561,565	-				-2.7
5,561,565	-				-1.6
5,561,565	-				-1.0
5,561,565	-				-1.5
5,561,565	-				-3.2
5,561,705	+				-0.5
5,561,706	-				-2.1
5,561,706	-				-0.8
5,561,754	+		Pf6N2E2_4810	0.16	-1.0
5,561,982	+		Pf6N2E2_4810	0.50	-1.3
5,561,983	-		Pf6N2E2_4810	0.50	-2.1
5,562,098	+		Pf6N2E2_4810	0.67	-2.7
5,562,126	+		Pf6N2E2_4810	0.71	-2.9
5,562,126	+		Pf6N2E2_4810	0.71	-2.4
5,562,126	+		Pf6N2E2_4810	0.71	-1.6
5,562,126	+		Pf6N2E2_4810	0.71	-1.4
5,562,126	+		Pf6N2E2_4810	0.71	-2.2
5,562,126	+		Pf6N2E2_4810	0.71	-0.7
5,562,126	+		Pf6N2E2_4810	0.71	-1.7
5,562,127	-		Pf6N2E2_4810	0.71	-0.9
5,562,127	-		Pf6N2E2_4810	0.71	-1.7
5,562,127	-		Pf6N2E2_4810	0.71	-2.9
5,562,127	-		Pf6N2E2_4810	0.71	-1.6
5,562,127	-		Pf6N2E2_4810	0.71	-2.6
5,562,321	+				+0.1
5,562,321	+				-2.9
5,562,321	+				-0.1
5,562,321	+				-1.3
5,562,321	+				-0.6
5,562,321	+				+1.1
5,562,321	+				+1.1
5,562,321	+				+0.1
5,562,321	+				-0.9
5,562,322	-				-0.9
5,562,322	-				-2.7
5,562,322	-				-0.9
5,562,322	-				-1.0
5,562,322	-				-1.2
5,562,322	-				-1.6
5,562,322	-				+0.4
5,562,322	-				-0.6
5,562,402	-				+0.2
5,562,404	+				+0.6
5,562,428	+				-0.6
5,562,659	+		Pf6N2E2_4811	0.34	-2.6
5,562,659	+		Pf6N2E2_4811	0.34	-2.8
5,562,659	+		Pf6N2E2_4811	0.34	-2.1
5,562,660	-		Pf6N2E2_4811	0.35	-1.4
5,562,660	-		Pf6N2E2_4811	0.35	+0.9
5,562,880	+		Pf6N2E2_4811	0.68	-0.7
5,562,880	+		Pf6N2E2_4811	0.68	-2.2
5,562,880	+		Pf6N2E2_4811	0.68	-1.5
5,562,881	-		Pf6N2E2_4811	0.68	+0.3
5,562,881	-		Pf6N2E2_4811	0.68	-0.3
5,563,158	+				+0.6
5,563,158	+				+0.4
5,563,158	+				+1.0
5,563,158	+				-0.9
5,563,158	+				-0.6
5,563,159	-				-1.3
5,563,159	-				-1.7
5,563,159	-				+0.7
5,563,263	+				-0.5
5,563,264	-				+0.7
5,563,264	-				-0.4