Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4790 and Pf6N2E2_4791 are separated by 127 nucleotides Pf6N2E2_4791 and Pf6N2E2_4792 are separated by 67 nucleotides
Pf6N2E2_4790 - sensory box/GGDEF domain/EAL domain protein, at 5,535,177 to 5,538,920
_4790
Pf6N2E2_4791 - RNA polymerase sigma factor RpoD, at 5,539,048 to 5,540,895
_4791
Pf6N2E2_4792 - DNA primase (EC 2.7.7.-), at 5,540,963 to 5,542,921
_4792
Position (kb)
5539
5540
5541 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5538.078 kb on + strand, within Pf6N2E2_4790 at 5538.112 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.234 kb on + strand, within Pf6N2E2_4790 at 5538.235 kb on - strand, within Pf6N2E2_4790 at 5538.235 kb on - strand, within Pf6N2E2_4790 at 5538.235 kb on - strand, within Pf6N2E2_4790 at 5538.298 kb on + strand, within Pf6N2E2_4790 at 5538.298 kb on + strand, within Pf6N2E2_4790 at 5538.298 kb on + strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.299 kb on - strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.466 kb on + strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.467 kb on - strand, within Pf6N2E2_4790 at 5538.497 kb on - strand, within Pf6N2E2_4790 at 5538.514 kb on + strand, within Pf6N2E2_4790 at 5538.514 kb on + strand, within Pf6N2E2_4790 at 5538.514 kb on + strand, within Pf6N2E2_4790 at 5538.575 kb on + strand at 5538.575 kb on + strand at 5538.587 kb on - strand at 5538.587 kb on - strand at 5538.630 kb on + strand at 5538.631 kb on - strand at 5538.631 kb on - strand at 5538.727 kb on + strand at 5538.727 kb on + strand at 5538.727 kb on + strand at 5538.727 kb on + strand at 5538.727 kb on + strand at 5538.728 kb on - strand at 5538.749 kb on - strand at 5538.960 kb on - strand at 5538.960 kb on - strand at 5538.960 kb on - strand at 5538.988 kb on - strand at 5539.002 kb on + strand at 5539.002 kb on + strand at 5539.002 kb on + strand at 5539.003 kb on - strand at 5539.003 kb on - strand at 5539.012 kb on + strand at 5539.013 kb on - strand at 5539.013 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,538,078 + Pf6N2E2_4790 0.77 -2.2 5,538,112 + Pf6N2E2_4790 0.78 +0.1 5,538,234 + Pf6N2E2_4790 0.82 -2.3 5,538,234 + Pf6N2E2_4790 0.82 -0.3 5,538,234 + Pf6N2E2_4790 0.82 -1.5 5,538,234 + Pf6N2E2_4790 0.82 +1.9 5,538,234 + Pf6N2E2_4790 0.82 +0.1 5,538,234 + Pf6N2E2_4790 0.82 +1.3 5,538,235 - Pf6N2E2_4790 0.82 -0.1 5,538,235 - Pf6N2E2_4790 0.82 -1.5 5,538,235 - Pf6N2E2_4790 0.82 -1.9 5,538,298 + Pf6N2E2_4790 0.83 -1.9 5,538,298 + Pf6N2E2_4790 0.83 +0.7 5,538,298 + Pf6N2E2_4790 0.83 +0.2 5,538,299 - Pf6N2E2_4790 0.83 +0.6 5,538,299 - Pf6N2E2_4790 0.83 -1.9 5,538,299 - Pf6N2E2_4790 0.83 +0.4 5,538,299 - Pf6N2E2_4790 0.83 -1.5 5,538,299 - Pf6N2E2_4790 0.83 +0.1 5,538,299 - Pf6N2E2_4790 0.83 +0.6 5,538,299 - Pf6N2E2_4790 0.83 -0.3 5,538,299 - Pf6N2E2_4790 0.83 +1.1 5,538,466 + Pf6N2E2_4790 0.88 +0.5 5,538,466 + Pf6N2E2_4790 0.88 +0.2 5,538,466 + Pf6N2E2_4790 0.88 +0.0 5,538,466 + Pf6N2E2_4790 0.88 -0.9 5,538,466 + Pf6N2E2_4790 0.88 +0.3 5,538,466 + Pf6N2E2_4790 0.88 +0.8 5,538,466 + Pf6N2E2_4790 0.88 +0.3 5,538,466 + Pf6N2E2_4790 0.88 +0.4 5,538,466 + Pf6N2E2_4790 0.88 +1.1 5,538,466 + Pf6N2E2_4790 0.88 +0.5 5,538,466 + Pf6N2E2_4790 0.88 +0.9 5,538,466 + Pf6N2E2_4790 0.88 -2.6 5,538,466 + Pf6N2E2_4790 0.88 +0.3 5,538,466 + Pf6N2E2_4790 0.88 -0.9 5,538,466 + Pf6N2E2_4790 0.88 -0.1 5,538,466 + Pf6N2E2_4790 0.88 +0.5 5,538,466 + Pf6N2E2_4790 0.88 +1.3 5,538,466 + Pf6N2E2_4790 0.88 -0.6 5,538,467 - Pf6N2E2_4790 0.88 -0.3 5,538,467 - Pf6N2E2_4790 0.88 +1.8 5,538,467 - Pf6N2E2_4790 0.88 +1.0 5,538,467 - Pf6N2E2_4790 0.88 +1.0 5,538,467 - Pf6N2E2_4790 0.88 +1.3 5,538,467 - Pf6N2E2_4790 0.88 -0.2 5,538,467 - Pf6N2E2_4790 0.88 -0.3 5,538,467 - Pf6N2E2_4790 0.88 +0.5 5,538,467 - Pf6N2E2_4790 0.88 -0.1 5,538,467 - Pf6N2E2_4790 0.88 +0.4 5,538,467 - Pf6N2E2_4790 0.88 -0.3 5,538,497 - Pf6N2E2_4790 0.89 -3.2 5,538,514 + Pf6N2E2_4790 0.89 -2.7 5,538,514 + Pf6N2E2_4790 0.89 -1.2 5,538,514 + Pf6N2E2_4790 0.89 -1.2 5,538,575 + -0.2 5,538,575 + +0.8 5,538,587 - +0.8 5,538,587 - +0.1 5,538,630 + +0.6 5,538,631 - -0.2 5,538,631 - +0.1 5,538,727 + -0.6 5,538,727 + +1.0 5,538,727 + -0.5 5,538,727 + +0.6 5,538,727 + -0.1 5,538,728 - -0.4 5,538,749 - +0.5 5,538,960 - +0.4 5,538,960 - +0.9 5,538,960 - -0.1 5,538,988 - -0.1 5,539,002 + +1.1 5,539,002 + -0.1 5,539,002 + -1.4 5,539,003 - +1.0 5,539,003 - +0.1 5,539,012 + +0.9 5,539,013 - -2.2 5,539,013 - +2.2
Or see this region's nucleotide sequence