Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4709 and Pf6N2E2_4710 overlap by 38 nucleotides Pf6N2E2_4710 and Pf6N2E2_4711 overlap by 27 nucleotides
Pf6N2E2_4709 - Cholesterol oxidase (EC 1.1.3.6), at 5,441,961 to 5,445,413
_4709
Pf6N2E2_4710 - hypothetical protein, at 5,445,376 to 5,445,594
_4710
Pf6N2E2_4711 - FIG00506946: hypothetical protein, at 5,445,568 to 5,447,436
_4711
Position (kb)
5445
5446 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5444.511 kb on - strand, within Pf6N2E2_4709 at 5444.511 kb on - strand, within Pf6N2E2_4709 at 5444.551 kb on - strand, within Pf6N2E2_4709 at 5444.568 kb on + strand, within Pf6N2E2_4709 at 5444.568 kb on + strand, within Pf6N2E2_4709 at 5444.569 kb on - strand, within Pf6N2E2_4709 at 5444.880 kb on - strand, within Pf6N2E2_4709 at 5444.934 kb on + strand, within Pf6N2E2_4709 at 5444.979 kb on + strand, within Pf6N2E2_4709 at 5444.979 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.018 kb on + strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.019 kb on - strand, within Pf6N2E2_4709 at 5445.028 kb on + strand, within Pf6N2E2_4709 at 5445.028 kb on + strand, within Pf6N2E2_4709 at 5445.168 kb on + strand at 5445.168 kb on + strand at 5445.168 kb on + strand at 5445.169 kb on - strand at 5445.169 kb on - strand at 5445.234 kb on + strand at 5445.234 kb on + strand at 5445.235 kb on - strand at 5445.235 kb on - strand at 5445.235 kb on - strand at 5445.261 kb on + strand at 5445.261 kb on + strand at 5445.261 kb on + strand at 5445.262 kb on - strand at 5445.262 kb on - strand at 5445.262 kb on - strand at 5445.279 kb on - strand at 5445.552 kb on + strand, within Pf6N2E2_4710 at 5445.552 kb on + strand, within Pf6N2E2_4710 at 5445.553 kb on - strand, within Pf6N2E2_4710 at 5445.577 kb on + strand at 5445.577 kb on + strand at 5445.577 kb on + strand at 5445.578 kb on - strand at 5445.578 kb on - strand at 5445.578 kb on - strand at 5445.751 kb on + strand at 5445.751 kb on + strand at 5445.751 kb on + strand at 5445.751 kb on + strand at 5445.751 kb on + strand at 5445.752 kb on - strand at 5445.752 kb on - strand at 5445.752 kb on - strand at 5445.841 kb on + strand, within Pf6N2E2_4711 at 5445.842 kb on - strand, within Pf6N2E2_4711 at 5445.842 kb on - strand, within Pf6N2E2_4711 at 5445.931 kb on + strand, within Pf6N2E2_4711 at 5445.931 kb on + strand, within Pf6N2E2_4711 at 5445.931 kb on + strand, within Pf6N2E2_4711 at 5446.091 kb on - strand, within Pf6N2E2_4711 at 5446.145 kb on - strand, within Pf6N2E2_4711 at 5446.151 kb on - strand, within Pf6N2E2_4711 at 5446.151 kb on - strand, within Pf6N2E2_4711 at 5446.151 kb on - strand, within Pf6N2E2_4711 at 5446.190 kb on - strand, within Pf6N2E2_4711 at 5446.190 kb on - strand, within Pf6N2E2_4711 at 5446.192 kb on + strand, within Pf6N2E2_4711 at 5446.192 kb on + strand, within Pf6N2E2_4711 at 5446.192 kb on + strand, within Pf6N2E2_4711 at 5446.192 kb on + strand, within Pf6N2E2_4711 at 5446.193 kb on - strand, within Pf6N2E2_4711 at 5446.193 kb on - strand, within Pf6N2E2_4711 at 5446.366 kb on + strand, within Pf6N2E2_4711 at 5446.453 kb on + strand, within Pf6N2E2_4711 at 5446.454 kb on - strand, within Pf6N2E2_4711 at 5446.454 kb on - strand, within Pf6N2E2_4711 at 5446.543 kb on + strand, within Pf6N2E2_4711 at 5446.544 kb on - strand, within Pf6N2E2_4711 at 5446.544 kb on - strand, within Pf6N2E2_4711 at 5446.544 kb on - strand, within Pf6N2E2_4711 at 5446.544 kb on - strand, within Pf6N2E2_4711 at 5446.544 kb on - strand, within Pf6N2E2_4711
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 5,444,511 - Pf6N2E2_4709 0.74 +1.2 5,444,511 - Pf6N2E2_4709 0.74 -0.3 5,444,551 - Pf6N2E2_4709 0.75 -1.2 5,444,568 + Pf6N2E2_4709 0.75 -0.5 5,444,568 + Pf6N2E2_4709 0.75 +0.4 5,444,569 - Pf6N2E2_4709 0.76 +1.6 5,444,880 - Pf6N2E2_4709 0.85 +0.1 5,444,934 + Pf6N2E2_4709 0.86 +0.1 5,444,979 + Pf6N2E2_4709 0.87 +0.3 5,444,979 + Pf6N2E2_4709 0.87 +1.9 5,445,018 + Pf6N2E2_4709 0.89 +1.9 5,445,018 + Pf6N2E2_4709 0.89 -0.3 5,445,018 + Pf6N2E2_4709 0.89 -0.7 5,445,018 + Pf6N2E2_4709 0.89 -2.0 5,445,018 + Pf6N2E2_4709 0.89 -1.7 5,445,018 + Pf6N2E2_4709 0.89 -0.8 5,445,019 - Pf6N2E2_4709 0.89 +0.8 5,445,019 - Pf6N2E2_4709 0.89 +1.1 5,445,019 - Pf6N2E2_4709 0.89 -1.7 5,445,019 - Pf6N2E2_4709 0.89 +0.9 5,445,019 - Pf6N2E2_4709 0.89 +1.3 5,445,019 - Pf6N2E2_4709 0.89 +2.1 5,445,019 - Pf6N2E2_4709 0.89 +0.0 5,445,019 - Pf6N2E2_4709 0.89 -0.8 5,445,028 + Pf6N2E2_4709 0.89 -1.3 5,445,028 + Pf6N2E2_4709 0.89 -1.1 5,445,168 + +1.9 5,445,168 + +0.6 5,445,168 + -0.1 5,445,169 - -0.9 5,445,169 - -2.4 5,445,234 + -0.6 5,445,234 + +0.4 5,445,235 - -0.1 5,445,235 - -0.3 5,445,235 - -0.7 5,445,261 + -0.2 5,445,261 + -1.4 5,445,261 + +0.3 5,445,262 - -0.7 5,445,262 - -1.3 5,445,262 - +0.2 5,445,279 - -3.4 5,445,552 + Pf6N2E2_4710 0.80 -2.2 5,445,552 + Pf6N2E2_4710 0.80 -3.2 5,445,553 - Pf6N2E2_4710 0.81 -1.6 5,445,577 + -0.2 5,445,577 + -0.0 5,445,577 + +0.6 5,445,578 - -0.1 5,445,578 - +0.4 5,445,578 - +0.6 5,445,751 + -0.3 5,445,751 + +0.7 5,445,751 + -0.9 5,445,751 + -1.6 5,445,751 + +0.3 5,445,752 - -2.1 5,445,752 - +0.3 5,445,752 - -1.1 5,445,841 + Pf6N2E2_4711 0.15 -0.1 5,445,842 - Pf6N2E2_4711 0.15 +0.6 5,445,842 - Pf6N2E2_4711 0.15 -0.6 5,445,931 + Pf6N2E2_4711 0.19 -2.3 5,445,931 + Pf6N2E2_4711 0.19 -1.8 5,445,931 + Pf6N2E2_4711 0.19 +0.4 5,446,091 - Pf6N2E2_4711 0.28 -1.2 5,446,145 - Pf6N2E2_4711 0.31 -0.7 5,446,151 - Pf6N2E2_4711 0.31 -0.8 5,446,151 - Pf6N2E2_4711 0.31 -1.0 5,446,151 - Pf6N2E2_4711 0.31 +0.6 5,446,190 - Pf6N2E2_4711 0.33 +1.6 5,446,190 - Pf6N2E2_4711 0.33 +0.3 5,446,192 + Pf6N2E2_4711 0.33 +0.1 5,446,192 + Pf6N2E2_4711 0.33 +2.1 5,446,192 + Pf6N2E2_4711 0.33 +1.0 5,446,192 + Pf6N2E2_4711 0.33 +0.6 5,446,193 - Pf6N2E2_4711 0.33 +2.3 5,446,193 - Pf6N2E2_4711 0.33 +1.4 5,446,366 + Pf6N2E2_4711 0.43 -0.1 5,446,453 + Pf6N2E2_4711 0.47 +1.0 5,446,454 - Pf6N2E2_4711 0.47 +1.2 5,446,454 - Pf6N2E2_4711 0.47 +0.9 5,446,543 + Pf6N2E2_4711 0.52 +0.6 5,446,544 - Pf6N2E2_4711 0.52 +0.0 5,446,544 - Pf6N2E2_4711 0.52 -1.0 5,446,544 - Pf6N2E2_4711 0.52 -0.5 5,446,544 - Pf6N2E2_4711 0.52 -0.0 5,446,544 - Pf6N2E2_4711 0.52 -2.1
Or see this region's nucleotide sequence